12/9/2 (Item 2 from file: 10)

3359461 20386158 Holding Library: AGL

Chronic staphylococcal osteomyelitis: a new experimental rat model

Spagnolo, N. Greco, F.; Rossi, A.; Ciolli, L.; Teti, A.; Posteraro, P.

Infection and immunity. Dec 1993. v. 61 (12) p. 5225‑5230.

A rat model of chronic staphylococcal osteomyelitis was developed. Fibrin glue (5 microliters) and Staphylococcus aureus [2x10(6) CFU/5 microliters] were inoculated into the proximal metaphysis of the tibia. The rats were killed at intervals of between 1 and 6 months, and the tibias were removed. Induced lesions were evaluated by radiographic, macroscopic, and histological examinations and bacterial counts. Roentgenograms revealed osteomyelitis in more than 90% of the tibias. Gross bone pathology revealed skeletal deformation, new bone formation, abscesses, and draining skin fistulas in more than 80% of cases. Histological examination revealed osteomyelitis in more than 90% of cases, and bacterial counts were positive in 86% of cases. Only fibrin glue (5 microliters) was inoculated into controls. Controls showed no osteomyelitic lesions, and counts were negative in seven of eight control tibias. The main feature of this model is the use of fibrin glue instead of the sclerosing agents and foreign bodies used in other models. The model reproduces lesions similar to those of human posttraumatic osteomyelitis and can be reliably used in pathophysiological and therapeutic studies.

Descriptors: rats ‑ disease models ‑ staphylococcus aureus ‑

osteomyelitis ;

Section Headings: L110 LABORATORY AND EXPERIMENTAL ANIMALS

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12/9/15 (Item 13 from file: 155)

A new model for posttraumatic osteomyelitis in rabbits.

Eerenberg JP; Patka P; Haarman HJ; Dwars BJ

J Invest Surg ( UNITED STATES ) Sep‑Oct 1994 , 7 (5) p453‑65

A new animal model for posttraumatic osteomyelitis was designed. This model mimics the pathogenesis of the human disease more accurately than models presently available. Femora of New Zealand white rabbits were exposed at the greater trochanter and a stainless steel rod was inserted into the marrow cavity. A Staphylococcus aureus suspension was placed in and around a bone defect, which was drilled midshaft. The disease was evaluated by clinical observation and roentgenographic, hematologic, bacteriologic, and histologic parameters. Osteomyelitis developed in all 24 infected rabbits. None of the five rabbits receiving only an intramedullary rod developed an osteomyelitis. This model proves that an experimental posttraumatic osteomyelitis associated with a foreign body can be reliably induced, even when no infection‑promoting chemical agents, small inoculum of bacteria, or minimal bone trauma is present.

Tags: Animal; Comparative Study; Female

Descriptors: *Disease Models, Animal; *Femur Neck‑‑Injuries‑‑IN; *Foreign Bodies ‑‑Complications‑‑CO; *Osteomyelitis; *Prostheses and Implants‑‑Adverse Effects‑‑AE; *Staphylococcal Infections ; Bone Marrow‑‑Injuries‑‑IN; Bone Marrow‑‑Microbiology‑‑MI; Bone Marrow ‑‑Pathology‑‑PA; Equipment Contamination; Femur Neck‑‑Surgery‑‑SU; Osteomyelitis‑‑Etiology‑‑ET; Osteomyelitis‑‑Pathology‑‑PA; Osteomyelitis ‑‑Radiography‑‑RA; Rabbits; Reoperation; Staphylococcal Infections ‑‑Etiology‑‑ET; Staphylococcal Infections‑‑Pathology‑‑PA; Staphylococcal Infections‑‑Radiography‑‑RA

MEDLINE(R) (Dialog« File 155): (c) format only 2000 Dialog Corporation. All

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12/9/48 (Item 46 from file: 155)

An experimental model of post‑traumatic osteomyelitis in rabbits.

Worlock P; Slack R; Harvey L; Mawhinney

Br J Exp Pathol ( ENGLAND ) Apr 1988 , 69 (2) p235‑44 , ISSN

An experimental model of a contaminated open fracture, using the tibia of male New Zealand white rabbits, is described. Post‑traumatic osteomyelitis can be reliably induced in this model, with no systemic ill‑effects. The characteristic bacteriological, radiological and histological findings are described. Inoculation of the fracture site with Staphylococcus aureus in a concentration of 10(6) bacteria caused osteomyelitis in two out of five rabbits. When the concentration of inoculum was increased to 10(7) organisms, osteomyelitis was seen in four out of five rabbits. No cases of infection were seen in the control animals. This is a simple and reliable model for studies into the prevention and treatment of post‑traumatic osteomyelitis.

Tags: Animal; Male; Support, Non‑U.S. Gov't

Descriptors: *Disease Models, Animal; *Fractures, Open‑‑Complications‑‑CO; *Osteomyelitis‑‑Etiology‑‑ET ; Bone and Bones‑‑Pathology‑‑PA; Fracture Fixation, Intramedullary; Neutrophils‑‑Pathology‑‑PA; Osteomyelitis‑‑Pathology‑‑PA; Osteomyelitis ‑‑Radiography‑‑RA; Rabbits; Staphylococcal Infections‑‑Complications‑‑CO; Tibial Fractures‑‑Complications‑‑CO; Tibial Fractures‑‑Radiography—RA

MEDLINE(R) (Dialog« File 155): (c) format only 2000 Dialog Corporation. All

rights reserved.

12/9/68 (Item 66 from file: 155)

03805552 82237670

[Posttraumatic osteomyelitis. Mode of infection, significance of metal

implants]

Posttraumatische Osteomyelitis. Infektionsmodus, Bedeutung der

Metallimplantation.

Passl R

Fortschr Med ( GERMANY, EAST ) May 20 1982 , 100 (19) p894‑7 ,

Guniea‑pigs of the Duncan‑Hartley strain were used in a model of posttraumatic osteomyelitis. One femur had been fractured with or without consequent metal implantation and infected subsequently with 10(7) or 10(5) staphylococcus aureus or E. coli. 85% of the animals infected with staph. aureus and 56% infected with E. coli developed osteomyelitis. Healing of infection occurred in 3 cases only and always in the absence of metal implants. All bone infection persisting for more than 3 months were also detectable after 12‑18 months. The assessment of opsonising factors revealed a long lasting deficiency in guinea‑pigs with metal implants.

Tags: Animal

Descriptors: *Bone Nails‑‑Adverse Effects‑‑AE; *Fracture Fixation, Internal‑‑Adverse Effects‑‑AE; *Osteomyelitis ; Disease Models, Animal; Escherichia coli‑‑Pathogenicity‑‑PY; Femoral Fractures‑‑Surgery‑‑SU; Guinea Pigs; Opsonins‑‑Analysis‑‑AN; Osteomyelitis ‑‑Etiology‑‑ET; Osteomyelitis‑‑Immunology‑‑IM; Phagocytosis; Staphylococcus aureus‑‑Pathogenicity‑‑PY; Virulence CAS Registry No.: 0 (Opsonins)