9/9/2 (Item 2 from file: 155)

8/9/2 (Item 2 from file: 155)

10238621 20083448

In vivo internalization of Staphylococcus aureus by embryonic chick

osteoblasts.

Reilly SS; Hudson MC; Kellam JF; Ramp WK

Bone ( UNITED STATES ) Jan 2000 , 26 (1) p63‑70 , ISSN 8756‑3282

Journal Code: ASR

Staphlylococcus aureus is the primary pathogen associated with osteomyelitis, an acute and recurrent bone disease. Internalization of S. aureus by cultured embryonic chick calvarial osteoblasts has been observed. The purpose of this study was to demonstrate that internalization of bacteria by embryonic chick calvarial and tibial osteoblasts occurs in vivo. Thus, internalization of S. aureus by osteoblasts in vivo augments the previous observation in vitro. This study has also shown that osteoblasts with few bacteria continue differentiating into osteocytes. Results of these experiments support the hypothesis that internalization of S. aureus by osteoblasts may play a role in the etiology of osteomyelitis.

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8/9/23 (Item 23 from file: 155)

08597058 97004970

Internalization of Staphylococcus aureus by cultured osteoblasts.

Hudson MC; Ramp WK; Nicholson NC; Williams AS; Nousiainen MT

Microb Pathog ( ENGLAND ) Dec 1995 , 19 (6) p409‑19 , ISSN

0882‑4010 Journal Code: MIC

Staphylococcus aureus is the most common cause of both acute and chronic osteomyelitis; however, the pathogenesis of osteomyelitis is poorly understood. We investigated the ability of S. aureus to associate with chick osteoblasts in culture and have demonstrated internalization of bacteria by the osteoblasts. Two strains of S. aureus were examined that were ingested by osteoblasts to different extents, suggesting strain differences in uptake. Initial association of S. aureus strains with osteoblasts was independent of the presence of matrix collagen produced by the osteoblasts. Internalization of bacteria required live osteoblasts, but not live S. aureus, indicating osteoblasts are active in ingesting the organisms. The bacteria were not killed by the osteoblasts, since viable bacteria were cultured several hours after ingestion.

Tags: Animal; Support, Non‑U.S. Gov't

Descriptors: *Osteoblasts‑‑Microbiology‑‑MI; *Staphylococcus

aureus‑‑Pathogenicity‑‑PY ; Bacterial Adhesion; Cells, Cultured;

Chickens; Endocytosis

MEDLINE(R) (Dialog« File 155): (c) format only 2000 Dialog Corporation. All

rights reserved.

8/9/47 (Item 47 from file: 155)

07491502 93145560

The effect of wound environment on the incidence of acute osteomyelitis.

Evans RP; Nelson CL; Harrison BH

Clin Orthop ( UNITED STATES ) Jan 1993 , (286) p289‑97 , ISSN

0009‑921X Journal Code: DFY

A model was developed to identify and compare the local wound factors that induce acute osteomyelitis in a prospective, controlled investigation. When compared with wounds containing either virulent bacteria or dead bone, statistical analysis disclosed a significant increase in the incidence of osteomyelitis when virulent bacteria and dead bone were combined. The incidence of osteomyelitis in wounds containing an inoculated, hematoma‑filled dead space was significantly less when compared with wounds containing dead bone and virulent bacteria. The incidence of osteomyelitis is significantly less when a nonvirulent strain of bacteria is substituted for a virulent strain. Although rigid internal fixation increased the incidence of osteomyelitis to 100% and long‑term antibiotic therapy decreased the incidence, these changes were not statistically significant. These data allow the authors to predict the relative risk of osteomyelitis when these wound factors are present. The prevention of osteomyelitis depends on the clinical identification and modification of these local wound factors.

Tags: Animal

Descriptors: *Models, Biological; *Osteomyelitis‑‑Physiopathology‑‑PP; *Wounds and Injuries‑‑Physiopathology‑‑PP ; Acute Disease; Ceftriaxone‑‑Therapeutic Use‑‑TU; Osteomyelitis‑‑Drug Therapy‑‑DT; Osteomyelitis‑‑Microbiology‑‑MI; Prospective Studies; Rabbits ; Risk; Staphylococcus aureus‑‑Pathogenicity‑‑PY; Virulence; Wounds and Injuries‑‑Microbiology‑‑MI CAS Registry No.: 73384‑59‑5 (Ceftriaxone)

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8/9/64 (Item 64 from file: 155)

06354701 90099794

Acute experimental osteomyelitis and abscesses: detection with MR imaging versus CT

Chandnani VP; Beltran J; Morris CS; Khalil SN; Mueller CF; Burk JM;

Bennett WF; Shaffer PB; Vasila MS; Reese J; et al

Radiology ( UNITED STATES ) Jan 1990 , 174 (1) p233‑6

Acute experimental osteomyelitis and abscesses were induced in the proximal tibia and surrounding soft tissues, respectively, in 67 New Zealand white rabbits. Fifty‑three rabbits were injected with a Staphylococcus aureus solution and 26, with sterile saline in tibial medullae and/or surrounding soft tissues. Contrast material‑enhanced computed tomography (CT) and magnetic resonance (MR) imaging were performed 7 days after inoculation. Immediately after imaging, the animals were killed and necropsy was performed. MR imaging was more sensitive than CT in the detection of osteomyelitis (94% vs 66%, P less than .025) and abscesses (97% vs 52%, P less than .001). MR imaging was equally specific as CT in the exclusion of osteomyelitis (93% vs 97%, chi 2 = 0) but less specific than CT in the exclusion of abscesses (77% vs 100%, P less than .025). The overall accuracy of MR imaging was somewhat, although not significantly, greater than that of CT in the detection of both osteomyelitis (93% vs 80%) and abscesses (87% vs 75%).

Tags: Animal; Comparative Study; Support, Non‑U.S. Gov't

Descriptors: *Abscess‑‑Diagnosis‑‑DI; *Magnetic Resonance Imaging; *Muscular Diseases ‑‑Diagnosis‑‑DI; *Osteomyelitis‑‑Diagnosis‑‑DI *Staphylococcal Infections ‑‑Diagnosis‑‑DI; *Tomography, X‑Ray Computed ; Rabbits; Tibia

MEDLINE(R) (Dialog« File 155): (c) format only 2000 Dialog Corporation. All