Fish Related Institutional Animal Care and Use Committees (IACUCs) Web Resources

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Protocol Forms          Guidelines          General Topics

Euthanasia and Endpoints

Laws, Regulations, and Governing Entities


Protocol Forms

General Methods for Zebrafish Care
Zebrafish Standard Protocol Form
            “The following PDF file (see note below) contains a template for animal use protocols
             involving zebrafish which has been approved by the veterinarian (OVSAC) and the animal
             use committee (IACUC) at the University of Oregon. It is provided to facilitate preparation
             of applications by users who must meet similar regulations. The text is also available on
             computer disk.”

Animal Use Application to the Institutional Animal Care & Use Committee (IACUC)
University of Oregon
Zebrafish Form

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99-10: New IACUC Application and USDA Policy 12 Compliance Requirements
            The new IACUC application complies with USDA policy 12. ... 2) Are there any
            alternative animal models (eg, invertebrates, fish, protozoa, etc.)? ...

AFS Policy Statement #16.  American Fisheries Society (AFS), American Society of Ichthyologists  and Herpetologists (ASIH), and American Institute of Fishery Research Biologists (AIFRE) (1987) Guidelines for the Use of Fishes in Field Research.  Approved Sept 1987, Winston-Salem, NC.  Published Mar-Apr 1988, Fisheries 13(2):16-23.

AFS Policy Statement #22.  Robinette, H Randall (Chair); Hynes, Julian; Parker, Nick C; Putz, Robert; Stevens, Robert E; and Stickney, Robert R (1990) ABBREVIATED, AFS Policy Statement #22: Commercial Aquaculture. Approved Aug 1990, Pittsburgh, PA. Published Jan-Feb 1991, Fisheries 15(2):12

AFS Policy Statement #30.  Kapuscinski, AR and EM Hallerman (1990) Responsible use of Fish and Other Aquatic Organisms.  Fisheries 15(4):2-5
            Catching and consuming fish are only parts of the value, especially in ... and federally
            mandated "institutional animal care and use committees" (IACUC), which are ...

CCAC Guidelines on: The Care and Use of Fish in Research, Teaching and Testing
            The second draft of the [guidelines] can be downloaded in MS Word format from this site.
            The final draft will be posted after all input from the second draft is taken into consideration. 

DeTolla LJ, Srinivas S, Whitaker BR, Andrews C, Hecker B, Kane AS, Reimschuessel R (1995)
Guidelines for the Care and Use of Fish in Research.  ILAR Journal 37(4) 

FSBI (2002) Fish Welfare. Briefing Paper 2, Fisheries Society of the British Isles, Granta Information Systems, 82A High Street, Sawston, Cambridge, CB2 4H, Tel/Fax: +44 (0) 1223 830665.  Email:

IACUC Guideline X:  Aquatic Guidelines
Penn State
            ... A density of more than 1.5 cm of fish per liter of water should not be ... Each tank
            must be identified with an identification number assigned by the IACUC ...

Schwedler TE, Johnson SK (2000) Responsible Care and Health Maintenance of Fish in Commercial Aquaculture.  Animal Welfare Information Center Bulletin, Winter1999/2000.  vol. 10, no. 3-4.

Thorsteinsson V (2002)  Tagging Methods for Stock Assessment and Research in Fisheries.  Report of Concerted Action FAIR CT.96.1394 (CATAG). Reykjavik. Marine Research Institute Technical Report (79), pp 179

Westerfield, M (2000)  The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish Danio (Brachydanio) rerio. 4th ed., Univ. of Oregon Press, Eugene

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General Topics

Rose JD (2002)  The Neurobehavioral Nature of Fishes and the Question of Awareness and Pain. Reviews in Fisheries Science. 10(1):1-38

Are Plants Making Fish “Happy”?

A dialog regarding the use of natural plants and an excellent response by Monte Westerfield (author of  The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish Danio (Brachydanio) rerio) regarding the potential introduction of pathogens into the system. 

... 25 Mar 1998 12:28 -0400 (EDT) From: Subject: Re: (Fwd) Are plants
making fish happy ... I would suggest you get help from your IACUC chairperson ...

AWIC Bulletin:  Wildlife Research and the IACUC
            For fish, our IACUC has determined this same stage of development to be the "buttoned-
            up" stage, or when the embryo has fully absorbed the yolk sac and must ...

University of Alaska, Fairbanks
              ... National Wildlife Health Center, USGS. United States Fish and Wildlife Service,
             USF&WS. National Marine Fisheries Service, NMFS. Other resources and IACUC sites. ... 

IACUC Zoonoses
Species Specific Fact Sheets

University of Pennsylvania
            click on Fish, Reptiles, and Amphibians for a comprehensive table of zoonoses
            ... Zoonoses (diseases which can be transmitted from animals to humans under natural
            conditions) by animal species: birds, dogs, cats, ferrets, fish, reptiles, and ...

Information Regarding the Use of Zebrafish at MBL
Institutional Animal Care & Use Committee

Marine Biological Laboratory, Woods Hole, MA
            ... Investigators bringing fish from an outside facility to the MBL must choose a
            method of care for the fish when making an application for IACUC approval. ...

Information Resources for Institutional Animal Care and Use Committees, 1985-1999, Revised
September 2000.  AWIC Resource Series No. 7 

Professional Guidelines
            ... NAL call number: QL55 A1I43 ... Descriptors: principal investigator, animal care
            personnel, IACUC members, overview of current uses of fish in research and
            commerce ...

National Resource for Zebrafish
Comparative Medicine Resources Directory
National Center for Research Resources, NIH

Northern Arizona University IACUC Self-Instruction Module for Certification in the
Humane Care and Use of Laboratory Animals
            ... c. State Game and Fish agencies require permits to trap or hold most wildlife species
            ... Check with the IACUC chair if you have any question regarding permits. ...

Veterinarians in Research Labs Address Conflicting Agendas
            ... I hope they were humane when they killed the fish."... of the Washington, DC-based
            Humane Society of the United States, for example, think IACUC veterinarians are ...

Zebrafish at UCL 


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Euthanasia and Endpoints

CCAC Guidelines on: Choosing an Appropriate Endpoint in Experiments Using Animals for
Research, Teaching and Testing

Acute Toxicity Testing in Fish
             ... justified for reasons of scientific necessity and approved by the institutional
            animal care and use committee (IACUC). ... Acute toxicity studies in fish ...

Euthanasia Guidelines
University of Minnesota

Euthanasia Protocol
SOP-160  Euthanasia
procedure with explicit IACUC approval. Cooling and/or freezing, liquid nitrogen.
            Total immersion of prenatal rats and mice or larval forms of fish in liquid ...

Guidelines for Euthanasia
University of California, Riverside

            Table of Euthanasia Methods

IACUC Guideline XI
Electrofishing Guidelines

Penn State  

Policy for Euthanasia of Research Animals
UCI Animal Subjects
            ... Fish, barbiturates inhalant anesthetics, CO2, Tricaine methane sulfonate (TMS, MS222)
            ... 2. All euthanasia methods must be described in an approved IACUC protocol. ...

UW Policy for the Euthanasia of Fish Species - 2002
            ... UW Policy for the Euthanasia of Fish Species - 2002. Approved July 18, 2002. ... A.
            Primary euthanasia methods for fish (methods that can be used alone) include: ...

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Laws, Regulations, and Governing Entities

IACUC Learning Module:  Laws and Regulations
Laws, Guidelines & Programs Affecting Animal Research

The University of Arizona
            IACUC Learning Module - Laws & Regulations ... Arizona Game & Fish and agencies
            such as Fish & Wildlife Service ...

Regulations, Guidelines, and Miscellaneous Recommendations
University of Idaho
             resource contains numerous links of interest!
            ... Fish & Wildlife, ... IACUCs, Institutional Animal Care and Use Committee Guidebook.
            Wildlife Research and the IACUC -
Animal Welfare Information Center ...

Regulations and Governing Bodies
Office of the University Veterinarian
University of Kentucky Research
            Federal Agencies, Laws, Regulations and Policies
             The Freedom of Information Act
             Independent National Regulatory Bodies and Professional Groups


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Updated: May 29, 2012