Bacteria
2007
Adjei, M.D.; Deck, J.; Heinze,
T.M.; Freeman, J.P.; Williams, A.J.; Sutherland, J.B. Identification of
metabolites produced from N-phenylpiperazine by Mycobacterium spp.
Journal of Industrial Microbiology and Biotechnology.
2007 Mar; 34 (3): 219-224. ISSN: 1367-5435
URL: http://dx.doi.org/10.1007/s10295-006-0189-x
NAL Call Number: QR53 .J68
Abstract: Mycobacterium sp. 7E1B1W and seven other mycobacterial
strains known to degrade hydrocarbons were investigated to determine their
ability to metabolize the piperazine ring, a substructure found in many drugs.
Cultures were grown at 30degrees C in tryptic soy broth and dosed with 3.1
mM N-phenylpiperazine hydrochloride; samples were removed at intervals and
extracted with ethyl acetate. Two metabolites were purified from each of
the extracts by high-performance liquid chromatography; they were identified
by mass spectrometry and superscript 1(BH nuclear
magnetic resonance spectroscopy as N-(2-anilinoethyl)acetamide and N-acetyl-N'-phenylpiperazine.
The results show that mycobacteria have the ability to acetylate piperazine
rings and cleave carbon-nitrogen bonds.
Descriptors: Mycobacterium strains, ability to metabolize piperazine,
broth culture, extracts via hplchromatography, acetylation of piperazine rings,
cleavage of carbon-nitrogen bonds.
Chitra, M.A.; Subodh Kishore
Lipoarabinomannan for the serological diagnosis of bovine
tuberculosis. Indian Veterinary Journal.
2007; 84 (2): 123-126. ISSN: 0019-6479
URL: www.indvetjournal.com
NAL Call Number: 41.8 IN2
Descriptors: cattle; lipoarabinomannan antigen; ELISA test; Mycobacterium
bovis identification, optical density compared to other mycobacterial
species, diagnostic test sensitivity, serodiagnosis in the field, compared
to PPD, CCF, CSA antigens.
Denis, M.; Keen,
D.L.; Parlane, N.A.; Storset, A.K.; Buddle, B.M. Bovine natural
killer cells restrict the replication of Mycobacterium bovis in bovine
macrophages and enhance IL-12 release by infected macrophages. Tuberculosis.
2007; 87 (1): 53-62. ISSN: 1472-9792
URL: http://www.sciencedirect.com/science/journal/14729792
Descriptors: blood monocyte-derived bovine macrophages, stimulated
NK cells and release interleukin-2 (IL-2), natural killer cells, innate bovine
resistance to virulent M. bovis, reduction of bacterial growth in macrophages.
Flynn, Robin
J.; Mannion, Celine; Golden, Olwen; Hacariz, Orcun; Mulcahy, Grace.
Experimental Fasciola hepatica infection alters responses to tests
used for diagnosis of bovine tuberculosis. Infection
and Immunity (IAI). 2007 Mar; 75 (3): 1373-1381. ISSN:
0019-9567
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: Fasciola hepatica is a prevalent helminth parasite
of livestock. Infection results in polarization of the host's immune response
and generation of type 2 helper (Th2) immune responses, which are known to
be inhibitory to Th1 responses. Bovine tuberculosis (BTB) is a bacterial
disease of economic and zoonotic importance. Control polices for this disease
rely on extensive annual testing and a test-and-slaughter policy. The correct
diagnosis of BTB relies on cell-mediated immune responses. We established
a model of coinfection of F. hepatica and Mycobacterium bovis
BCG to examine the impact of helminth infection on correct diagnosis. We
found the predictive capacity of tests to be compromised in coinfected animals
and that F. hepatica infection altered macrophage function. Interleukin-4
and gamma interferon expression in whole-blood lymphocytes restimulated in
vitro with M. bovis antigen was also altered in coinfected animals.
These results raise the question of whether F. hepatica infection
can affect the predictive capacity of tests for the diagnosis of BTB and possibly
also influence susceptibility to BTB and other bacterial
diseases. Further studies on the interplay between helminth infection and
BTB are warranted.
Descriptors: livestock, Fasciola hepatica, liver fluke, Mycobacterium
bovis BCG, co-infection of helminths and bacteria, question whether bovine
tuberculosis testing compromised, suggest further studies.
Fujiwara, Nagatoshi; Nakata, Noboru;
Maeda, Shinji; Naka, Takashi; Doe, Matsumi; Yano, Ikuya; Kobayashi, Kazuo.
Structural characterization of a specific glycopeptidolipid containing
a novel N-acyl-deoxy sugar from Mycobactetium intracellulare serotype
7 and genetic analysis of its glycosylation pathway Journal of Bacteriology.
2007; 189 (3): 1099-1108. ISSN: 0021-9193
URL: http://jb.asm.org/
NAL Call Number: 448.3 J82
Descriptors: Mycobacterium avium, Mycobacterium intracellulare complex (MAC) respiratory and lymphatic pathogen, humans and animals, produce
polar glycopeptidolipids, serotype specific antigenicity, structural characterization,
glycosylation pathway, serovars.
Gannon, B.W.;
Hayes, C.M.; Roe, J.M. Survival rate of airborne
Mycobacterium bovis. Research in Veterinary
Science. 2007; 82 (2): 169-172. ISSN: 0034-5288
URL: http://www.sciencedirect.com/science/journal/00345288
NAL Call Number: 41.8 R312
Descriptors: survival time of aerosolized, Mycobacterium bovis,
half life of 1.5 hours, airborne transmission, cattle infection,
may be principle route of infection.
Gong, Qiang; Liu, Si Guo; Guo,
She Ping; Wang, Chun Lai; Wang, Yong; Liu, Jian Dong; Zhao, Kun; Chi, Lei;
Kong, Xian Gang. Immunogenicity of DNA vaccine containing
esat-6 gene or mpb70-mpb83 fusion gene from Mycobacterium bovis.
Veterinary Science in
URL: http://www.zgsykx.com/
Descriptors: Mycobacterium bovis Vallee III, mycobacterial
infections, DNA vaccine, fragments of esat-6 and mpb70-mpb83, antigenicity,
cloning vectors, immunity reactions, immunogens, immunological reactions,
mycobacterial infections, BALB mice.
Marsh, I.B.;
Whittington, R.J. Genomic diversity in Mycobacterium avium:
Single nucleotide polymorphisms between the S and C strains of M-avium
subsp paratuberculosis and with M-a. avium.
Molecular and Cellular Probes. 2007; 21(1): 66-75.
ISSN: 0890-8508
URL: http://www.sciencedirect.com/science/journal/08908508
Descriptors: sheep; cattle; Mycobacterium avium paratuberculosis;
strain C; strain S; Mycobacterium avium avium; amino acid sequence;
nucleotide sequence; genomic diversity; species comparison; GenBank sequence
numbers; 12,117 bp of sequence representing 26 loci across 25 genes; 11 SNPs
were identified between the S and C strains in eight genes: hsp65, sodA, dnaA,
dnaN, recF, gyrB, inhA, and pks8.
Descriptors: cattle, acute signs of bovine respiratory disease, sampling
with swabs, 220 pathogens isolated, Arcanobacterium pyogenes, Histophilus,
Mycobacterium bovis, Pasteurella haemolytica, Pasteurella
multocida, sensitivity to antiobiotics, florfenicol, tilmicosin, tulathromycin,
tetracycline, 8 European countries.
Ren, Huiping; Dover, Lynn G.;
Islam, Salim T.; Alexander, David C.; Chen, Jeffrey M.; Besra, Gurdyal S.;
Liu, Jun. Identification of the lipooligosaccharide
biosynthetic gene cluster from Mycobacterium marinum. Molecular
Microbiology. 2007 Mar; 63 (5): 1345-1359. ISSN: 0950-382X
.
URL: http://dx.doi.org/10.1111/j.1365-2958.2007.05603.x
NAL Call Number: QR74.M65
Abstract: Lipooligosaccharides (LOSs) are antigenic glycolipids that
are present in some species of Mycobacterium including the Canetti
strain of M. tuberculosis. The core LOS structures from several mycobacterial
organisms have been established, but the biosynthetic pathways of LOSs remain
unknown. In this study, we describe two transposon insertion mutants of M.
marinum that exhibit altered colony morphology. Cell wall analysis reveals
that the MRS1271 mutant is defective in the synthesis of LOS-II, whereas the
MRS1178 mutant accumulates an intermediate between LOS-I and -II. The genetic
lesions were localized to two genes, MM2309 and MM2332. MM2309 encodes a UDP-glucose
dehydrogenase that is involved in the synthesis of d-xylose. MM2332 is predicted
to encode a decarboxylase. These two genes and a previously identified losA
gene are localized in a gene cluster likely to be involved in the biosynthesis
of LOSs. Our results also show that LOSs play an important role in sliding
motility, biofilm formation, and infection of host macrophages. Taken together,
our studies have identified, for the first time, a LOS biosynthetic locus.
This is an important step in assessing the differential distribution of LOSs
among Mycobacterium species and understanding the role of LOSs in mycobacterial
virulence.
Descriptors: Mycobacterium marinum, Lipooligosaccharides, biosynthetic
pathways, transposon mutants, biosynthetic locus.
Sharma, S.;
Mallick, G.P.; Rishendra Verma; Ray, S.K. Polymerase chain reaction
(PCR) amplification of IS6110 sequences to detect Mycobacterium tuberculosis
complex from formalin-fixed paraffin-embedded tissues of deer (Axis axis).
Veterinary Research Communications.
2007; 31 (1): 17-21. ISSN: 0165-7380
URL: http://springerlink.metapress.com/link.asp?id=103009
NAL Call Number: SF601.V38:
Descriptors: Axix deer (Cervus axis), diagnostic test, PCR
IS6110 sequences, fixed tissue samples, Mycobacterium bovis, Mycobacterium
tuberculosis,
Sreenu, V.B.;
Pankaj Kumar; Javaregowda Nagaraju; Nagarajaram, H.A. Simple sequence repeats
in mycobacterial genomes. Journal of Biosciences.
2007; 32 (1): 3-5. ISSN: print 0250-5991, e-0973-7138
URL: http://www.ias.ac.in/jbiosci
Descriptors: Mycobacterium, Mycobacterium avium, Mycobacterium
bovis, Mycobacterium tuberculosis, DNA insertion elements, insertion
sequences, microsatellites, simple sequence repeats, mobile genetic elements,
mobile sequences, transposons.
Sreenu, V.B.;
Pankaj Kumar; Javaregowda Nagaraju; Nagarajaram, H.A. Microsatellite
polymorphism across the M. tuberculosis and M. bovis genomes:
implications on genome evolution and plasticity. BMC
Genomics. 2006; 7 (78): (10 April 2006). ISSN: 1471-2164
URL: http://www.biomedcentral.com/content/pdf/1471-2164-7-78.pdf
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis,
DNA transcription, microsatellite indel mutations, novel functions, plasticity
to the mycobacterial genomes, phenotypic variability, polymorphism.
Thanky, Niren R.; Young, Douglas
B.; Robertson, Brian D. Unusual features of the cell cycle in mycobacteria:
polar-restricted growth and the snapping-model of cell division. Tuberculosis
(
Descriptors: cell divisions, mycobacterial growth, cell lengths, Mycobacterium
bovis BCG, Mycobacterium smegmatis, Mycobacterium tuberculosis,
peptidoglycan, cell poles, V shape.
Turenne, C.Y.; Wallace, R., Jr;
Behr,M.A. Mycobacterium
avium in the postgenomic era. Clinical Microbiology Reviews. 2007; 20 (2): 205-229.
ISSN: 0893-8512
URL: http://cmr.asm.org/cgi/content/abstract/20/2/205
Descriptors: Mycobacterium avium complex, genomic data, detection
and diagnosis, genomic variability of Mycobacterium subset relationships,
fundamental differences and ability to cause disease.
Vega-Manriquez,
X.; Lopez-Vidal, Y.; Moran, J.;
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: Mycobacterium tuberculosis complex species survive
and replicate in phagosomes of the host cell. Cell death (CD) has been highlighted
as one of the probable outcomes in this host-pathogen interaction. Previously,
our group demonstrated macrophage apoptosis as a consequence of Mycobacterium
bovis infection. In this study, we aimed to identify the contribution
of apoptotic effector elements in M. bovis-induced CD. Bovine macrophages
were either infected with M. bovis (multiplicity of infection, 10:1)
or treated with an M. bovis cell extract (CFE). Structural changes
compatible with CD were evaluated. Chromatin condensation was increased three
times by the CFE. On the other hand, a terminal deoxynucleotidyltransferase-mediated
dUTP-biotin nick end labeling (TUNEL) assay demonstrated that levels of DNA
fragmentation induced by M. bovis and CFE were 53.7% +/- 24% and 38.9%
+/- 14%, respectively, whereas control cells had a basal proportion of 8.9%
+/- 4.1%. Rates of DNA fragmentation were unaffected by the presence of the
pan-caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp (z-VAD). Cells treated
with 100 (So(Bg of CFE for 12 h had a fivefold decrease in the level of mitochondrial
outer membrane permeabilization compared to that of untreated cells. Neither
M. bovis infection nor CFE treatment induced activation of caspase
3, 8, or 9. Translocation of apoptosis-inducing factor (AIF) to the nucleus
was identified in 32% +/- 3.5% and 26.3% +/- 4.9% of M. bovis-infected
and CFE-treated cells, respectively. Incubation of macrophages with z-VAD
prior to infection did not alter the percentage of cells showing AIF translocation.
Our data suggest that M. bovis-induced CD in bovine macrophages is
caspase independent with AIF participation.
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis,
cell death, macrophage apoptosis, bacterial infection, structural changes
of cell death, chromatin condensation, DNA fragmentation, mitochondrial outer
membrane permeabilization, apoptosis inducing factor, caspase independent.
Waters, W.R.;
Nonnecke, B.J.;
URL: http://www.sciencedirect.com/science/journal/03781135
NAL Call Number: SF601.V44
Abstract: The BovigamTM assay is approved for use within the
Descriptors: young
NAL Call Number: 49 J82
Abstract: Tissue banking and animal cloning represent a powerful tool
for conserving and regenerating valuable animal genomes. Here we report an
example involving cattle and the rescue of a genome affording natural disease
resistance. During the course of a 2-decade study involving the phenotypic
and genotypic analysis for the functional and genetic basis of natural disease
resistance against bovine brucellosis, a foundation sire was identified and
confirmed to be genetically resistant to Brucella abortus. This unique
animal was utilized extensively in numerous animal breeding studies to further
characterize the genetic basis for natural disease resistance. The bull died
in 1996 of natural causes, and no semen was available for AI, resulting in
the loss of this valuable genome. Fibroblast cell lines had been established
in 1985, cryopreserved, and stored in liquid nitrogen for future genetic analysis.
Therefore, we decided to utilize these cells for somatic cell nuclear transfer
to attempt the production of a cloned bull and salvage this valuable genotype.
Embryos were produced by somatic cell nuclear transfer and transferred to
20 recipient cows, 10 of which became pregnant as determined by ultrasound
at d 40 of gestation. One calf survived to term. At present, the cloned
bull is 4.5 yr old and appears completely normal as determined by physical
examination and blood chemistry. Furthermore, in vitro assays performed to
date indicate this bull is naturally resistant to B. abortus, Mycobacterium
bovis, and Salmonella typhimurium, as was the original genetic
donor.
Descriptors: cattle, tissue banking, natural disease resistance in
a bull, fibroblast cell line, cryopreserved, cloning of germplasm, 1 viable
offspring, resistance to B. abortus, Mycobacterium bovis, Salmonella
typhimurium.
Yip, Marcus J.; Porter, Jessica
L.; Fyfe, Janet A.M.; Lavender, Caroline J.; Portaels, Francoise; Rhodes,
Martha; Kator, Howard; Colorni, Angelo; Jenkin, Grant A.; Stinear, Tim. Evolution of Mycobacterium ulcerans and other mycolactone-producing
mycobacteria from a common Mycobacterium marinum progenitor.
Journal of Bacteriology. 2007; 189 (5): 2021-2029.
ISSN: 0021-9193
URL: http://jb.asm.org/
NAL Call Number: 448.3 J82
Descriptors: evolution of cytotoxic polyketide mycolactones, Mycobacterium
ulcerans, Buruli ulcers, Mycobacterium marinum progenitor of mycolactones,
multiple genetic methods, multilocus sequence analysis, DNA-DNA hybridization,
plasmid acquisition, ecotypes, pathogens of ectotherms and endotherms, mammals,
frogs, fish.
2006
Adaekambi, Toeidi;
Ben Salah, Skandar; Khlif, Mohamed; Raoult, Didier; Drancourt, Michel.
Survival of environmental mycobacteria in Acanthamoeba
polyphaga. Applied and
Environmental Microbiology (AEM). 2006 Sept; 72 (9): 5974-5981.
ISSN: 0099-2240
URL: http://aem.asm.org/contents-by-date.0.shtml
NAL Call Number:
448.3 Ap5
Abstract: Free-living amoebae in water are hosts to many bacterial
species living in such an environment. Such an association enables bacteria
to select virulence factors and survive in adverse conditions. Waterborne
mycobacteria (WBM) are important sources of community- and hospital-acquired
outbreaks of nontuberculosis mycobacterial infections. However, the interactions
between WBM and free-living amoebae in water have been demonstrated for only
few Mycobacterium spp. We investigated the ability of a number (n
= 26) of Mycobacterium spp. to survive in the trophozoites and cysts
of Acanthamoeba polyphaga. All the species tested entered the trophozoites
of A. polyphaga and survived at this location over a period of 5 days.
Moreover, all Mycobacterium spp. survived inside cysts for a period of 15
days. Intracellular Mycobacterium spp. within amoeba cysts survived
when exposed to free chlorine (15 mg/liter) for 24 h. These data document
the interactions between free-living amoebae and the majority of waterborne
Mycobacterium spp. Further studies are required to examine the effects
of various germicidal agents on the survival of WBM in an aquatic environment.
Descriptors: Acanthamoeba polyphaga free living amoebae, survival
of Mycobacterium in A. polyphaga cysts, source of waterborne
Mycobacterium infections.
Akey, David; Martins, Alexandra;
Aniukwu, Jideofor; Glickman, Michael S.; Shuman, Stewart; Berger, James M.
Crystal Structure and nonhomologous end-joining function
of the ligase component of Mycobacterium DNA ligase D. Journal
of Biological Chemistry. 2006 May 12; 281(19): 13412-13423. ISSN:
0021-9258
URL: http://www.jbc.org/
NAL Call Number: 381 J824
Abstract: DNA ligase D (LigD) is a large polyfunctional enzyme involved
in nonhomologous end-joining (NHEJ) in mycobacteria. LigD consists of a C-terminal
ATP-dependent ligase domain fused to upstream polymerase and phosphoesterase
modules. Here we report the 2.4 eA crystal structure of the ligase domain
of Mycobacterium LigD, captured as the covalent ligase-AMP intermediate
with a divalent metal in the active site. A chloride anion on the protein
surface coordinated by the ribose 3'-OH and caged by arginine and lysine side
chains is a putative mimetic of the 5'-phosphate at a DNA nick. Structure-guided
mutational analysis revealed distinct requirements for the adenylylation and
end-sealing reactions catalyzed by LigD. We found that a mutation of Mycobacterium
LigD that ablates only ligase activity results in decreased fidelity of NHEJ
in vivo and a strong bias of mutagenic events toward deletions instead of
insertions at the sealed DNA ends. This phenotype contrasts with the increased
fidelity of double-strand break repair in [Delta]ligD
cells or in a strain in which only the polymerase function of LigD is defective.
We surmise that the signature error-prone quality of bacterial NHEJ in vivo
arises from a dynamic balance between the end-remodeling and end-sealing steps.
Descriptors: Mycobacterium, DNA ligaseD. crystal
structure, biochemistry, polyfunctional enzyme, end remodeling, end sealing.
Allix, Caroline; Walravens, Karl;
Saegerman, Claude; Godfroid, Jacques; Supply, Philip; Fauville-Dufaux, Maryse.
Evaluation of the epidemiological relevance of varable
number tandem-repeat genotyping of Mycobacterium bovis and comparison
of the method with IS6110 restriction fragment fength polymorphism analysis
and spoligotyping. Journal of Clinical
Microbiology. 2006 June; 44 (6): 1951-1962. ISSN: 0095-1137.
E-ISSN: 1098-660X
URL: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1489394
NAL Call Number: QR46.J6
Abstract: Sources of Mycobacterium bovis contamination remain
unclear for many cases of animal and human disease. A major limitation is
the lack of sufficiently informative or epidemiologically well evaluated molecular
methods for typing. Here, we report an evaluation of a high-throughput method
based on 29 mycobacterial interspersed repetitive unit-variable-number tandem-repeat
(MIRU-VNTR) loci to genotype 127 M. bovis isolates from cattle from
77 different Belgian farms, representative of a nationwide collection obtained
from 1995 to 2003. MIRU-VNTR stability was demonstrated by analyzing a series
of 74 isolates in total, obtained from different animals from a single farm
or from different farms with an identified epidemiological link. The genotyping
results and the genotypic diversity (h) were compared with those obtained
by IS6110 restriction fragment length polymorphism (RFLP) analysis and spoligotyping.
Among 68 isolates with no known epidemiological link, MIRU-VNTR typing discriminated
better than either RFLP analysis or spoligotyping, with isolates taken individually
(32 versus 16 and 17 genotypes; h = 0.91 versus 0.73 and 0.85, respectively)
or in combination (32 versus 28 genotypes; h = 0.91 versus 0.92). Maximal
resolution was already achieved with a subset of 9 loci. The observed congruence
of the genetic relationships based on IS6110 RFLP analysis, spoligotyping,
and MIRU-VNTR markers is consistent with a clonal population structure of
M. bovis. These results support MIRU-VNTR typing as a convenient and
discriminatory technique for analysis of the population structure of M.
bovis in much greater detail and for addressing some still unresolved
issues in the epidemiology of the pathogen.
Descriptors: Mycobacterium bovis, cattle isolates, animal bacterial
pathogen, epidemiology, discriminatory technique for population analysis,
29 mycobacterial interspersed repetitive unit-variable-number tandem-repeat
(MIRU-VNTR) loci to genotype 127.
Arraiz, N.;
Romay, Z.; Faria, N.; Mujica, D. Identificacion diferencial de
aislados clinicos de Mycobacterium tuberculosis y Mycobacterium
bovis por un ensayo de RCP multiple. [Differential
identification of Mycobacterium tuberculosis and Mycobacterium bovis
clinical isolates by multiplex PCR assay.] Revista Cientifica, Facultad
de Ciencias Veterinarias, Universidad del Zulia.
2006; 16 (6): 622-628. ISSN: 0798-2259. Note: In Spanish with an English
summary.
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis,
efficacy of PCR to differentiate Mycobacterium species, targeted Rv0577
and Rv1510 gene sequences, zoonotic infections.
Bartos, Milan; Hlozek, Pavel;
Svastova, Petra; Dvorska, Lenka; Bull, Tim; Matlova, Ludmila; Parmova, Ilona;
Kuhn, Isolde; Stubbs, Janine; Moravkova, Monika; Kintr, Jaromir; Beran, Vladimir;
Melicharek, Ivan; Ocepek, Matjaz; Pavlik, Ivo. Identification of members of Mycobacterium avium species
by Accu-Probes, serotyping, and single IS900, IS901, IS1245 and IS901-flanking
region PCR with internal standards. Journal
of Microbiological Methods. 2006; 64(3): 333-345. ISSN: 0167-7012
URL:http://www.elsevier.com/wps/find/journaldescription.cws_home/506034/description#description
NAL Call Number: QR65.J68
Descriptors: Mycobacterium avium species, Mycobacterium
avium ssp. paratuberculosis (n = 961), Mycobacterium avium avium
(n = 677), Mycobacterium avium silvaticum (n = 5), Mycobacterium
avium hominissuis (n = 1566), Mycobacterium tuberculosis, Mycobacterium
bovis (n = 13), Mycobacterium bovis BCG (n = 4), Mycobacterium
caprae (n = 10), Mycobacterium intracellulare (n = 60), atypical
mycobacteria (n = 256) including Mycobacterium fortuitum, Mycobacterium
chelonae, Mycobacterium scrofulaceum, Mycobacterium
gastri, insertion sequences IS900, IS901, IS1245, and flanking region
(FR300) of IS901, PCR of alfalfa genome segment and inserted into plasmid
vector, recombinant plasmids, internal amplicons, PCR typing compared with
serotyping and Accu-Probes analyses in selected field isolates.
Cadmus, Simeon; Palmer, Si; Okker,
Melissa; Dale, James; Gover, Karen; Smith, Noel; Jahans, Keith; Hewinson,
R. Glyn; Gordon, Stephen V. Molecular analysis of human
and bovine tubercle bacilli from a local setting in
URL: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1489394
NAL Call Number: QR46.J6
Abstract: To establish a molecular epidemiological baseline for the
strains causing tuberculosis in
Descriptors: humans, cattle, tubercular bacilli, Mycobacterium
africanum, Mycobacterium bovis, Mycobacterium tuberculosis,
molecular analysis of Mycobacterium tuberculosis complex strains, spoligotyping
and variable-number tandem-repeat analysis,
Chambers, M.A.;
Gavier-Widen, D.; Hewinson, R.G. Histopathogenesis
of experimental Mycobacterium bovis infection in mice.
Research in Veterinary Science. 2006.
80 (1): 62-70. ISSN: 0034-5288
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/623070/description#description
NAL Call Number: 41.8 R312
Descriptors: mice, cattle, badgers, Mycobacterium bovis, animal
disease models, bovine tuberculosis, histopathology, pathogenesis, virulence,
lesions animal, disease severity, host-pathogen relationships, vaccination,
pathogenicity, model validation.
Chilima, Benson
Z.; Clark, Ian M.; Floyd,
URL: http://www.pubmedcentral.nih.gov/tocrender.fcgi?action=archive&journal=83
NAL Call Number: 448.3 AP5
Abstract: The genus includes many species that are commonly found in
the environment (in soil and water or associated with plants and animals),
as well as species that are responsible for two major human diseases, tuberculosis
(Mycobacterium tuberculosis) and leprosy (Mycobacterium leprae).
The distribution of environmental mycobacteria was investigated in the context
of a long-term study of leprosy, tuberculosis, Mycobacterium bovis
BCG vaccination, and the responses of individuals to various mycobacterial
antigens in Karonga District, northern
Descriptors: soil bacteria, Mycobacterium, Mycobacterium
fortuitum, cell culture, differential staining, decontamination, polymerase
chain reaction, species diversity, ribosomal RNA, genes, nucleotide sequences,
phylogeny, molecular sequence data, decontamination culture, Malawi.
Chitra, M. Ananda;
Kishore, Subodh. Effect of mycobacterial lipoarabinomannan
on interleukin-2 production by bovine lymphocytes. Indian Veterinary Journal. 2006; 83 (7): 703-707. ISSN:
0019-6479
NAL Call Number: 41.8 IN2
Descriptors: Mycobacterium bovis, lipoarabinomannan (LAM),
major antigen of mycobacterial envelope, immunological activities, cytokines,
lymphocyte proliferation, induction of interleukin 2.
Collins, Desmond
M.; Skou, Bronwyn; White, Stefan; Bassett, Shalome; Collins, Lauren; For,
Raewyn; Hurr, Kathryn; Hotter, Grant; de Lisle, Geoffrey W. Generation
of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis
for discovery of novel vaccine candidates. Infection
and Immunity. 2005; 73 (4): 2379-2386. ISSN: 0019-9567
URL: http://www.pubmedcentral.nih.gov/tocrender.fcgi?iid=117182
NAL Call Number: QR1.I57
Abstract: Mycobacterium bovis, a member of the Mycobacterium
tuberculosis complex, has a particularly wide host range and causes tuberculosis
in most mammals, including humans. A signature tag mutagenesis approach,
which employed illegitimate recombination and infection of guinea pigs, was
applied to M. bovis to discover genes important for virulence and to
find potential vaccine candidates. Fifteen attenuated mutants were identified,
four of which produced no lesions when inoculated separately into guinea pigs.
One of these four mutants had nine deleted genes including mmpL4 and sigK
and, in guinea pigs with aerosol challenge, provided protection against tuberculosis
at least equal to that of M. bovis BCG. Seven mutants had mutations
near the esxA (esat-6) locus, and immunoblot analysis of these confirmed the
essential role of other genes at this locus in the secretion of EsxA (ESAT-6)
and EsxB (CFP10). Mutations in the eight other attenuated mutants were widely
spread through the chromosome and included pks1, which is naturally inactivated
in clinical strains of M. tuberculosis. Many genes identified were
different from those found by signature tag mutagenesis of M. tuberculosis
by use of a mouse infection model and illustrate how the use of different
approaches enables identification of a wider range of attenuating mutants.
Descriptors: Mycobacterium bovis, attenuated mutants, illegitimate
recombination and infection of guinea pigs.
Cosma, Christine
L.; Klein, Kathryn; Kim, Rosa; Beery, Dana; Ramakrishnan, Lalita. Mycobacterium
marinum Erp is a virulence determinant required for cell wall integrity
and intracellular survival. Infection and Immunity
(IAI). 2006 June; 74 (6): 3125-3133. ISSN: 0019-9567
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: The Mycobacterium tuberculosis exported repetitive
protein (Erp) is a virulence determinant required for growth in cultured macrophages
and in vivo. To better understand the role of Erp in Mycobacterium
pathogenesis, we generated a mutation in the Erp homologue of Mycobacterium
marinum, a close genetic relative of M. tuberculosis. Erp-deficient
M. marinum was growth attenuated in cultured macrophage monolayers
and during chronic granulomatous infection of leopard frogs, suggesting that
Erp function is similarly required for the virulence of both M. tuberculosis
and M. marinum. To pinpoint the step in infection at which Erp is
required, we utilized a zebrafish embryo infection model that allows M.
marinum infections to be visualized in real-time, comparing the Erp-deficient
strain to a [Delta]RD1 mutant whose stage of attenuation
was previously characterized in zebrafish embryos. A detailed microscopic
examination of infected embryos revealed that bacteria lacking Erp were compromised
very early in infection, failing to grow and/or survive upon phagocytosis
by host macrophages. In contrast, [Delta]RD1 mutant
bacteria grow normally in macrophages but fail to induce host macrophage aggregation
and subsequent cell-to-cell spread. Consistent with these in vivo findings,
erp-deficient but not RD1-deficient bacteria exhibited permeability defects
in vitro, which may be responsible for their specific failure to survive in
host macrophages.
Descriptors: Mycobacterium tuberculosis, Mycobacterium marinum,
exported repetitive protein, virulence determinant, cultured macrophages,
invivo, pathogensis, infection of leopard frogs, infected embryos, early infections,
permeability.
Costello, E.;
Flynn, O.; Quigley, F.; O'Grady, D.;
URL: http://veterinaryrecord.bvapublications.com/archive/
NAL Call Number: 41.8 V641
Descriptors: Mycobacterium bovis isolates from badgers, tissue
sampling of 2310 animals, RFLP analysis with IS6110, polymorphic GC-rich sequence
(PGRS), direct repeat sequence (DR) probes, 398 isolates, 52 RFLP types identifies,
movement of badgers between territories,
Costello, E.;
Flynn, O.; Quigley, F.; O'Grady, D.;
URL: http://veterinaryrecord.bvapublications.com/archive/
NAL Call Number: 41.8 V641
Descriptors: Mycobacterium bovis isolates from badgers, tissue
sampling of 2310 animals, RFLP analysis with IS6110, polymorphic GC-rich sequence
(PGRS), direct repeat sequence (DR) probes, 398 isolates, 52 RFLP types identifies,
movement of badgers between territories,
Courtenay, O.; Reilly, L.A.; Sweeney,
F.P.; Hibberd, V.; Bryan, S.; Ul Hassan, A.; Newman, C.; Macdonald, D.W.;
Delahay, R.J.; Wilson, G.J.; Wellington, E.M.H. Is Mycobacterium bovis
in the environment important for the persistence of bovine tuberculosis?
Biology Letters. 2006; 2 (3): 460-462.
ISSN: 1744-9561
URL: http://www.pubs.royalsoc.ac.uk/biol_lett
Descriptors: badgers (Meles meles), cattle, Mycobacterium
bovis, prevalence of pathogen in environment, detectability of M. bovis,
badger setts and latrines, environmental reservoir, endemic on cattle farms,
Britain.
Cruz, Andrea; Khader, Shabaana
A; Torrado, Egidio; Fraga, Alexandra; Pearl, John E; Pedrosa, Jorge; Cooper,
Andrea M.; Castro, Antonio G. Cutting edge: IFN-gamma regulates the induction
and expansion of IL-17-producing CD4 T cells during mycobacterial infection.
Journal of Immunology. 2006; 177 (3):
1416-1420. ISSN: 0022-1767.
URL: http://www.jimmunol.org
NAL Call Number: 448.8 J8232
Descriptors: Mycobacterium bovis bacille Calmette Guerin, IFN-gamma
deficient mice, IL 17 producing T cells, IL-12 and IL-23 from bone-marrow-derived
dendritic cells, changes in experession levels, counter regulation pathway,
effects on immune response.
Daly, M.; Diegel, K.L.; Fitzgerald,
S.D.; Schooley, A.; Berry, D.E.; Kaneene, J.B. Patterns of antimicrobial susceptibility in
URL: http://jvdi.org/
NAL Call Number: SF774.J68
Descriptors: Mycobacterium bovis, bacterial isolates, wildlife
and bovine sources, susceptibility to antibacterial compounds.
Endsley, Janice
J.; Endsley, Mark A; Estes, D Mark. Bovine natural killer cells
acquire cytotoxic/effector activity following activation with IL-12/15 and
reduce Mycobacterium bovis BCG in infected macrophages. Journal
of Leukocyte Biology. 2006; 79 (1): 71-79. ISSN: 0741-5400
URL: http://www.jleukbio.org/
NAL Call Number: QP185.R4
Descriptors: blood, blood and lymphatics, nervous system, macrophage,
immune system, monocytes, leukocytes, T lymphocytes, CD4 positive T cells,
bovine natural-killer-cells: NK cells, CD3, mRNA, IL-2, IL-12, IL-15, CD8,
CD25, CD94, NKp46, p46, CD244, CD56, neural adhesion molecule, granulysin,
perforin, Mycobacterium bovis bacillus Calmette Guerin.
URL: http://dx.doi.org/10.1111/j.1439-0450.2006.00921.x
NAL Call Number: 41.8 Z52
Abstract: Standards of the German Association of Veterinary Medicine
(DVG) for the evaluation of chemical disinfectants were used to assess the
anti-microbial efficacy of electrolysed oxidizing water (EOW). Enterococcus
faecium, Mycobacterium avium subspecies avium, Proteus
mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans
were exposed to anode EOW (pH, 3.0 * left-pointing-double-angle * 0.1;
oxidation-reduction potential (ORP), +1100 * left-pointing-double-angle *
50 mV; free chlorine, 400 * left-pointing-double-angle * 20 mg/l Cl subscript
2(B) and combined EOW (7 : 3 anode : cathode, v/v; pH, 8.3 * left-pointing-double-angle
* 0.1; ORP, 930-950 mV; free chlorine, 271 * left-pointing-double-angle *
20 mg/l Cl subscript 2(B). In water of standardized hardness (WSH), all bacterial
strains were completely inactivated by a 30 min exposure to maximum 10.0%
anode EOW ([approximately]40.0 mg/l Cl subscript 2(B) or 50.0% combined EOW
([approximately]135.5 mg/l Cl subscript 2(B). The sensitivity ranking order
for anode EOW to the bacterial test strains was P. mirabilis > S.
aureus > M. avium ssp. avium > E. faecium >
P. aeruginosa. P. mirabilis and S. aureus decreased
to undetectable levels after 5 min of exposure to 7.5% anode EOW ([approximately]30.0
mg/l Cl subscript 2(B). Candida albicans was completely inactivated
by a 5-min exposure to 5.0% anode EOW. Both, anode and combined EOW exhibited
no anti-microbial activities in standardized nutrient broth or after addition
of 20.0% bovine serum to the WSH. Further research is necessary to evaluate
the efficacy of EOW as a disinfectant under operating conditions in animal
production facilities.
Descriptors: cattle, animal pathogens, disinfectants, antimicrobial
agents, oxidants, chlorine, duration, nutrient solutions, blood serum, water-hardness,
Enterococcus faecium, Mycobacterium avium ssp. avium,
Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus
aureus, electrolysed oxidizing water, oxidation resistance, anodes, cathodes.
Food and Agriculture
Organization. Capacity building for surveillance
and control of zoonotic diseases, FAO/WHO/OIE Expert and Technical Consultation,
URL: http://www.fao.org
Abstract: This proceeding contains 14 papers. This publication is
intended to assist veterinary public health services in Developing Countries
and countries in transition in the implementation of capacity-building programmes
on surveillance and control of zoonotic diseases. Specific recommendations
were made on implementation of surveillance methodologies for zoonotic diseases.
There is a special emphasis on Developing Countries. The topics include:
recommendations for training programs in surveillance methodologies at veterinary
and para-veterinary levels; surveillance program in taeniasis/cysticercosis;
capacity building for the surveillance, prevention and control of BSE; control
of zoonotic disease under emergency conditions; surveillance and control programs
in brucellosis, Mycobacterium bovis, tuberculosis, anthrax, salmonellosis
and other foodborne pathogens; surveillance, early weaning and early reaction
to zoonoses outbreaks; and surveillance approaches in antimicrobial resistance.
Descriptors: animal health, training programs, disease surveillance
programs, major bacterial diseases, parasites, Burcella, Mycobacterium
bovis, BSE, Developing Countries.
Gao,
Lian Yong; Pak, Melissa;
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: The ability to invade and grow in macrophages is necessary
for Mycobacterium tuberculosis to cause disease. We have found a Mycobacterium
marinum locus of two genes that is required for both invasion and intracellular
survival in macrophages. The genes were designated iipA (mycobacterial invasion
and intracellular persistence) and iipB. The iip mutant, which
was created by insertion of a kanamycin resistance gene cassette at the 5'
region of iipA, was completely avirulent to zebra fish. Expression of the
M. tuberculosis orthologue of iipA, Rv1477, fully complemented the
iip mutant for infectivity in vivo, as well as for invasion and intracellular
persistence in macrophages. In contrast, the iipB orthologue, Rv1478, only
partially complemented the iip mutant in vivo and restored invasion but not
intracellular growth in macrophages. While IipA and IipB differ at their
N termini, they are highly similar throughout their C-terminal NLPC_p60 domains.
The p60 domain of Rv1478 is fully functional to replace that of Rv1477, suggesting
that the N-terminal sequence of Rv1477 is required for full virulence in vivo
and in macrophages. Further mutations demonstrated that both Arg-Gly-Asp
(RGD) and Asp-Cys-Ser-Gly (DCSG) sequences in the p60 domain are required
for function. The iip mutant exhibited increased susceptibility to antibiotics
and lysozyme and failed to fully separate daughter cells in liquid culture,
suggesting a role for iip genes in cell wall structure and function. Altogether,
these studies demonstrate an essential role for a p60-containing protein,
IipA, in the pathogenesis of M. marinum infection.
Descriptors: Mycobacterium marinum, genes for invasion and
survival in macrophages, iipA, iipB, mutants.
Godden, S.;
McMartin, S.; Feirtag, J.; Stabel, J.; Bey, R.; Goyal, S.; Metzger, L.; Fetrow,
J.; Wells, S.; Chester-Jones, H. Heat-treatment of bovine colostrum. II: Effects of
heating duration on pathogen viability and immunoglobulin G. Journal
of Dairy Science. 2006 Sept; 89 (9): 3476-3483. ISSN: 0022-0302
NAL Call Number: 44.8 J822
Abstract: Batches (30-L) of first-milking bovine colostrum, inoculated
with Mycoplasma bovis (10 superscript 8(B cfu/mL), Listeria monocytogenes
(10 superscript 6(B cfu/mL), Escherichia coli O157:H7 (10 superscript
6(B cfu/mL), Salmonella enteritidis (10 superscript 6(B cfu/mL), and
Mycobacterium avium subsp. paratuberculosis (Map; 10 superscript
3(B cfu/mL), were heat-treated at 60AC for 120 min in a commercial on-farm
batch pasteurizer system. Duplicate 50-mL subsamples of colostrum were collected
at 15-min intervals throughout the heat-treatment process for the purpose
of bacterial culture and for measurement of IgG concentration (mg/mL) and
antibody activity [log subscript 2(B(bovine viral diarrhea virus type 1 serum
neutralization titer)]. Four replicate batches of colostrum were run for
each of the 5 pathogens studied. There was no effect of heating moderate-
to high-quality colostrum at 60AC for at least 120 min on mean IgG concentration
(pre = 60.5 mg/mL; post = 59.1 mg/mL). Similarly, there was no effect of
heat-treatment on the mean log subscript 2(B bovine viral diarrhea virus type
1 serum neutralization titer (pre = 12.3; post = 12.0). Viable M. bovis,
L. monocytogenes, E. coli O157:H7,
and S. enteritidis added to colostrum could not be detected after the
colostrum was heat-treated at 60AC for 30 min. Average bacteria counts showed
that Map was not detected when batches were heated at 60AC for 60 min. Although
the authors believe that heat-treating colostrum at 60AC for 60 min should
be sufficient to eliminate Map from colostrum in most situations, further
research is needed to determine whether these findings may be replicated,
given that variability was observed in Map culture results.
Descriptors: first milking colostrum, inoculation with Mycoplasma
bovis, Mycobacterium avium paratuberculosis, Salmonells enteritidis, Listeria
monocytogenes, Escherichia coli, heat treatment process to inactivate
the pathogens.
Hao, Jun Feng; Zhao,
De Ming. Cloning and prokaryotic expression of
Mycobacterium bovis MB1916c gene. Journal
of
NAL Call Number: S19-C58
Descriptors: Mycobacterium bovis, amplification of MB1916 gene,
PCR technique.
Harris, N. Beth;
Payeur, Janet B.; Kapur, Vivek; Sreevatsan, Srinand. Short-Sequence-Repeat
analysis of Mycobacterium avium subsp. paratuberculosis and
Mycobacterium avium subsp. avium isolates collected from animals
throughout the
URL: http://jcm.asm.org/
NAL Call Number: QR46 .J6
Abstract: We analyzed the multilocus short sequence repeats (SSRs)
of 211 and 56 isolates of Mycobacterium avium subsp. paratuberculosis
and M. avium subsp. avium, respectively. The M. avium subsp.
paratuberculosis isolates could be differentiated into 61 genotypes.
The M. avium subsp. avium isolates showed limited diversity.
These SSRs are stable and suitable for studying the molecular epidemiology
of M. avium subsp. paratuberculosis.
Descriptors: Mycobacterium avium subsp. paratuberculosis,
M. avium subsp. avium, isolate identification, multilocus short
sequence repeats, diversity, molecular epidemiology.
Hervas-Stubbs, Sandra; Majlessi,
Laleh; Simsova, Marcela; Morova, Jana; Rojas, Marie-Jesus; Nouz_e,-Clemence;
Brodin, Priscille; Sebo, Peter; Leclerc, Claude. High frequency of CD4[superscript +] T cells specific for the TB10.4 protein
correlates with protection against Mycobacterium tuberculosis infection.
Infection and immunity (IAI).
2006; 74: (6): 3396-3407. ISSN: 0019-9567
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: TB10.4 is a newly identified antigen of Mycobacterium
tuberculosis recognized by human and murine T cells upon mycobacterial
infection. Here, we show that immunization with Mycobacterium bovis BCG
induces a strong, genetically controlled, Th1 immune response against TB10.4
in mice. BALB/c and C57BL/6 strains behave as high and low responders to
TB10.4 protein, respectively. The TB10.4:74-88 peptide was identified as an immunodominant CD4+
T-cell epitope for H-2d mice. Since recent results, as well as the present
study, have raised interest in TB10.4 as a subunit vaccine, we analyzed immune
responses induced by this antigen delivered by a new vector, the adenylate
cyclase (CyaA) of Bordetella pertussis. CyaA is able to target dendritic
cells and to deliver CD4[superscript +] or CD8+
T-cell epitopes to the major histocompatibility complex class II/I molecule
presentation pathways, triggering specific Th1 or cytotoxic T-lymphocyte (CTL)
responses. Several CyaA harboring either the entire TB10.4 protein or various
subfragments containing the TB10.4:20-28 CTL epitope were shown to induce
TB10.4-specific Th1 CD4+ and CD8+ T-cell responses.
However, none of the recombinant CyaA, injected in the absence of adjuvant,
was able to induce protection against M. tuberculosis infection. In
contrast, TB10.4 protein administered with a cocktail of strong adjuvants
that triggered a strong Th1 CD4+ T-cell response induced significant
protection against M. tuberculosis challenge. These results confirm
the potential value of the TB10.4 protein as a candidate vaccine and show
that the presence of high frequencies of CD4+ T cells specific
to this strong immunogen correlates with protection against M. tuberculosis
infection.
Descriptors: mice, Mycobacterium bovis, Mycobacterium tuberculosis,
TB10.4, newly identified antigen, possible vaccination candidate, immune
response in mice, CD4+ T cells, CD8+
T cells.
Hewinson, R.G.;
Vordermeier, H.M.; Smith, N.H.; Gordon, S.V. Recent advances in
our knowledge of Mycobacterium bovis: a feeling for the organism. Veterinary
Microbiology. 2006 Feb. 25; 112 (2-4): 127-139. ISSN: 0378-1135.
Note: Paper presented at the 4th International Conference on Mycobacterium
bovis, Held August 22-26, 2005,
URL: www.sciencedirect.com/science/journal/03781135
NAL Call Number: SF601.V44
Abstract: Significant and rapid progress has been made in our knowledge
and understanding of Mycobacterium bovis since the last international
M. bovis conference 5 years ago. Much of this progress has been underpinned
by the completion of the genome sequence. This important milestone has catalysed
research into the development of a number of improved tools with which to
combat bovine tuberculosis. In this article we will review recent progress
made in the development of these tools and in our understanding of the organism,
its evolution and spread. Comparison of the genome sequence with those of
other members of the Mycobacterium tuberculosis complex has enabled
insights into the evolution of M. bovis. This analysis also indicates
that the M. tuberculosis complex have the propensity to adapt to new
host species. The use of high throughput molecular typing methods has revealed
that the recent bovine tuberculosis epidemic in
Descriptors: cattle, Mycobacterium bovis, animal pathogenic
bacteria, bovine tuberculosis, literature reviews, genomics, nucleotide sequences,
microbial genetics, genome, evolution, host range, adaptation, disease outbreaks,
genetic drift, bacterial antigens, BCG vaccine, vaccination, molecular sequence
data.
Hicks, D.J.; Johnson, L.; Mitchell,
S.M.; Gough, J.; Cooley, W.A.; La-Ragione, R.M.; Spencer, Y.I.; Wangoo, A.
Evaluation of zinc salt based fixatives for preserving antigenic determinants
for immunohistochemical demonstration of murine immune system cell marker.
Biotechnic and Histochemistry. 2006; 81(1): 23-30.
ISSN: 1052-0295
URL: http://www.informaworld.com/smpp/title~content=t713692932
NAL Call Number: QH613.B56
Descriptors: immunohistochemical techniques antigen, cytokine and cytomorphological
markers; fixatives; mouse models for Mycobacterium bovis infection;
tissues from RIII mice; zinc salt fixative; buffered formalin; tested CD3,
CD4, CD8, CD45, CD54, F4/80, Interferon-gamma, MIP2.
Hines, N.; Payeur,
J.B.; Hoffman, L.J. Comparison of the recovery of Mycobacterium
bovis isolates using the BACTEC MGIT 960 system, BACTEC 460 system, and
Middlebrook 7H10 and 7H11 solid media. Journal of Veterinary Diagnostic Investigation. 2006
May; 18 (3): 243-250. ISSN: 1040-6387
URL: http://jvdi.org/
NAL Call Number: SF774.J68
Descriptors: cattle, lymph nodes, Mycobacterium bovis, animal
pathogenic bacteria, bovine tuberculosis, strains, pathogen identification,
diagnosis, culture media, tissue analysis, niacin, nitrates, microbial contamination,
disease detection, new methods.
Hogarth, P.J.;
Hewinson, R.G.; Vordermeier, H.M. Development of vaccines against bovine tuberculosis.
Journal of Pharmacy and Pharmacology. 2006; 58 (6):
749-757. ISSN: 0022-3573
URL: http://www.pharmpress.com/jpp
Descriptors: buffalo, cattle, Mycobacterium bovis, zoonotic
disease, disease control, review, vaccine research,
Horwitz, Marcus
A.; Harth, Guenter; Dillon, Barbara Jane; Maslesa-Galic, Sasa. A
novel live recombinant mycobacterial vaccine against bovine tuberculosis more
potent than BCG. Vaccine.
2006; 24 (10): 1593-1600. ISSN: 0264-410X
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/30521/description#description
Descriptors: vaccination, cattle, other domesticated animal diseases,
wild animal as disease reservoirs, Mycobacterium bovis, live
recombinant vaccine, rBCG30 expresses large amounts of the Mycobacterium
tuberculosis, 30kDa major secretory protein, more efficacious against
bovine tuberculosis than BCG, aerosol challenge.
Jiang Xiu yun;
Wang, Chun feng; Wang, Chun fang; Zhang, Peng ju; He, Zhao yang. Cloning
and expression of Mycobacterium bovis secreted protein MPB83
in Escherichia coli. Journal of Biochemistry
and Molecular Biology. 2006; 39 (1): 22-25. ISSN: 1225-8687
Descriptors: Mycobacterium bovis Vallee111, gene encoding
MPB83, PCR amplification, pGEM-T vector, cloning plasmid pGEM T 83 was constructed,
BamH1 and EcoRI digestion, purified MPB83 gene was subcloned, prokaryotic
expression vector pET28a-83 was constructed, transformed into competence Escherichia
coli BL21 (DE3), 26 kDa exogenous protein observed on SDS-PAGE, possible
subunit vaccine, DNA vaccine of MPB83 gene.
Jiang, XiuYun;
Wang, ChunFeng; Wang, ChunFang; He, ZhaoYang. Cloning and expression
of Mycobacterium bovis secreted protein Ag85B in E. coli.
Chinese Journal of Veterinary Science. 2006;
26(1): 51-54. ISSN: 1005-4545. Note: In Chinese with an English summary.
Descriptors: cattle, Escherichia coli, Mycobacterium tuberculosis,
DNA cloning, identify, gene expression, Clone vector pGEM-T-85B, secreted
protein Ag85B from Mycobacterium bovis Vallee 111, PCR, diagnosis,
plasmid containing pET28a-85B transformed into E. coli BL21(DE3).
Juan, L. de; Alvarez, J.; Aranaz,
A.; Bezos, J.; Romero, B.; Mateos, A.; Dominguez, L.; Travnicek, M. Epizootologia
a diagnostika mykobakterialnych infekcii.
Descriptors: diagnostic techniques, epidemiology, Mycobacterium
bovis, Mycobacterium caprae, clinical picture, main pathological
changes, culture protocol, PCR identification, tuberculin test, gamma interferon
tests.
Kubica, Tanja;
Agzamova, Rimma; Wright, Abigail; Rakishev, Galimzhan; Ruesch-Gerdes Sabine;
Niemann, Stefan. Mycobacterium bovis isolates with M.
tuberculosis specific characteristics. Emerging
Infectious Diseases. 2006; 12 (5): 763-765. ISSN: 1080-6040.
URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?linkbar=plain&db=journals&term=1080-6040
NAL Call Number: RA648.5.E46
Descriptors: Mycobacterium bovis strains, some characteristics
of M. tuberculosis, zoonotic isolates, humans, intermediate characteristics,
Lazzaro, B.P.;
Galac, M.R. Disease pathology: wasting energy fighting infection.
Current Biology. 2006; 16 (22): R964-R965.
ISSN: 0960-9822
URL: http://www.current-biology.com
Abstract: Drosophila melanogaster infected with Mycobacterium
marinum suffer metabolic wasting similar to that observed in humans suffering
from tuberculosis. This wasting is linked to insulin signalling and hastens
host death..
Descriptors: Drosophila melanogaster, effects of infection,
wasting disease, Mycobacterium marinum.
Lee, Keun Wook; Jung, Jinwon;
Lee, Younghee; Kim, Tae Yoon; Choi, Soo Young; Park, Jinseu; Kim, Doo Sik;
Kwon, Hyung Joo. Immunostimulatory oligodeoxynucleotide isolated from
genome wide screening of Mycobacterium bovis chromosomal DNA. Molecular
Immunology. 2006; 43 (13): 2107-2118. ISSN: 0161-5890.
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/253/description
NAL Call Number: 448.3 IM62
Descriptors: bacterial DNA, immunostimulatory activity, Mycobacterium
bovis, computer analysis of bacterial genome, activation of the NF-kappa
Li, Jing Jing; Zhao, De Ming;
Xu, Guang Xian; Zhou, Xiang Mei; Yin, Xiao Min. Cloning and expression
of Mycobacterium bovis secreted protein MPB83 in Escherichia coli.
Journal of
NAL Call Number: S19.C58
Descriptors: Mycobacterium bovis, cloning of the MPB83 gene,
analysis of its expression, SDS-PAGE and western blotting technique, possible
diagnostic tool.
Lu, Jun Peng; Luo,Man
Lin; Song,Yan Hua; Zou,Wei Li. Expression of MPT83 gene
from Mycobacterium bovis and purification of its recombinant protein.
Veterinary Science in
URL: http://www.zgsykx.com/
Descriptors: Mycobacterium bovis, DAN extraction, MPT83 gene
fragment amplified via PCR, cloned into plasmid pET-32a(+)
, transformed into BL21(DE3), recombinant protein.
Li, Rui Fang;
Qin, Ai Jian; Xu, Jin Jun. Preparation of the
specific monoclonal antibody against bovine gamma -interferon and its properties.
Chinese Journal of Zoonoses. 2006; 22
(8): 755-758. ISSN: 1002-2694. Note: In Chinese with an English summary.
Descriptors: cattle, Mycobacterium bovis, monoclonial, antibody,
fusion SP2/0cells and immunized mice spleen cells, immunogen on BALB/cmice,
BovIFN-gamma 4A3 BovIFN-gamma4G5, use for surveillance and control of TB in
milk cows.
Marcondes, A.G.; Shikama, M. de
L.M.; Vasconcellos, S.A.; Benites, N.R.; Morais, Z.M de; Roxo, E.; Dias, R.A.;
Leao, S.L.P.C.; Pinheiro, S.R. Comparacao entre a tecnica
de cultivo em camada delgada de agar Middlebrook 7H11 e meio de Stonebrink
para isolamento de Mycobacterium bovis em amostras de campo.
[Microcolony detection of Mycobacterium bovis in
Middlebrook 7H11 thin layer culture.] Brazilian
Journal of Veterinary Research and Animal Science. 2006; 43 (3):
362-369. ISSN: 1413-9596. Note: In Portuguese with an English summary.
Descriptors: bacterial morphology, Mycobacterium bovis AN5,
Mycobacterium tuberculosis H37Rv, cultivation technique in thin layer
of Middlebrook 7H11 (TL7H11).
Marri, Pradeep
Reddy; Bannantine, John P.; Golding, Geoffrey B. Comparative
genomics of metabolic pathways in Mycobacterium species: gene duplication,
gene decay and lateral gene transfer. FEMS Microbiology
Reviews. 2006 Nov; 30 (6): 906-925. ISSN: 0168-6445
URL: http://dx.doi.org/10.1111/j.1574-6976.2006.00041.x
NAL Call Number: QR1.F46
Abstract: The genus Mycobacterium comprises significant pathogenic
species that infect both humans and animals. One species within this genus,
Mycobacterium tuberculosis, is the primary killer of humans resulting
from bacterial infections. Five mycobacterial genomes belonging to four different
species (M. tuberculosis, Mycobacterium bovis, Mycobacterium
leprae and Mycobacterium avium ssp. paratuberculosis) have
been sequenced to date and another 14 mycobacterial genomes are at various
stages of completion. A comparative analysis of the gene products of key
metabolic pathways revealed that the major differences among these species
are in the gene products constituting the cell wall and the gene families
encoding the acidic glycine-rich (PE/PPE/PGRS) proteins. Mycobacterium
leprae has evolved by retaining a minimal gene set for most of the gene
families, whereas M. avium ssp. paratuberculosis has acquired
some of the virulence factors by lateral gene transfer.
Descriptors: Mycobacterium species, comparative genomics, biochemical
pathways, pathogenicity.
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/638428/description?navopenmenu=-2
Descriptors: mouse disease model, BALB/c mice TB resistant, DBA/2
mice TB susceptible, Mycobacterium tuberculosis, Mycobacterium bovis,
both susceptible to M. bovis, intravenous route, progressive infection,
pathology in kidneys, adrenal glands.
Megyeri, Klara; Buzas, Krisztina;
Miczak, Andrds; Buzas, Edit; Kovacs, Lrdnd; Seprenyi, Gyrgy; Falus, Andras;
Mandi, Yvette. The role of histamine in the intracellular
survival of Mycobacterium bovis BCG. Microbes
and Infection. 2006; 8 (4): 1035-1044. ISSN: 1286-4579.
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/601557/description#description
NAL Call Number: QR180.M53
Descriptors: intracellular survival of Mycobacterium bovis
BCG, murine bone marrow macrophages, wild type (WT) mice, histidine-decarboxylase
knock-out [HDC (-/-)] mice, comparison study, histamine may moderate IL 18
production, immune protection.
Moisan, Jacques; Thuraisingam,
Thusanth; Henault, Jill; De Sanctis, Juan.; Radzioch,
Danuta. Role of SLC11A1 (formerly NRAMP1) in regulation of signal transduction
induced by Toll-like receptor 7 ligands. FEMS Immunology
and Medical Microbiology. 2006; 47 (1): 138-147. ISSN: 0928-8244.
URL: http://www.blackwellpublishing.com/journal.asp?ref=0928-8244&site=1
NAL Call Number: QR180.F46
Descriptors: treatment for infectious and allergic diseases, toll-like
receptor ligands, TLR, Mycobacterium bovis BCG infection, synthetic
TLR 7 ligand, splenic bacterial load, mouse model, macrophage cell lines of
B10.A(Nramp1(r)) and B10.A(Nramp1(-/-)) mice, role for NRAMP1 in modulating
p38 MAPK and PKC zeta activity, reduced cytokine induction by TLR7 ligands.
More, S.J.;
Collins, J.D.; Gormley, E.; Good, M.; Skuce, R.A.; Pollock, J.M. 4th
International Conference on Mycobacterium bovis: workshop reports.
Veterinary Microbiology. 2006; 112 (2/4):
383-391. ISSN: 0378-1135. Note: Special issue: S.J. More; J.D. Collins;
E. Gormley; M. Good; R.A. Skuce; J.M. Pollock (editors). Proceedings
of the 4th International Conference on Mycobacterium bovis,
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/503320/description#description
NAL Call Number: SF601.V44
Descriptors: conference workshop reports, policy, strategy, Mycobacterium
bovis, disease control, disease eradication programs, diagnosis, molecular
epidemiology, wild animals as disease reservoirs, vaccines, vaccination of
animals, cattle, livestock.
Morita, Yasu S.; Sena, Chubert
B.C.; Waller, Ross F.; Kurokawa, Ken; Sernee, M. Fleur; Nakatani, Fumiki;
Haites, Ruth E.; Billman-Jacobe, Helen; McConville, Malcolm J.; Maeda, Yusuke;
Kinoshita, Taroh PimE Is a polyprenol-phosphate-mannose-dependent mannosyltransferase
that transfers the fifth mannose of phosphatidylinositol mannoside in mycobacteria.
Journal of Biological Chemistry. 2006
Sept 1; 281 (35): 25143-25155. ISSN: 0021-9258
URL: http://www.jbc.org/
NAL Call Number: 381 J824
Abstract: Phosphatidylinositol mannosides (PIMs) are a major class
of glycolipids in all mycobacteria. AcPIM2, a dimannosyl PIM, is both an
end product and a precursor for polar PIMs, such as hexamannosyl PIM (AcPIM6)
and the major cell wall lipoglycan, lipoarabinomannan (LAM). The mannosyltransferases
that convert AcPIM2 to AcPIM6 or LAM are dependent on polyprenol-phosphate-mannose
(PPM), but have not yet been characterized. Here, we identified a gene, termed
pimE that is present in all mycobacteria, and is required for AcPIM6 biosynthesis.
PimE was initially identified based on homology with eukaryotic PIG-M mannosyltransferases.
PimE-deleted Mycobacterium smegmatis was defective in AcPIM6 synthesis,
and accumulated the tetramannosyl PIM, AcPIM4. Loss of PimE had no affect
on cell growth or viability, or the biosynthesis of other intracellular and
cell wall glycans. However, changes in cell wall hydrophobicity and plasma
membrane organization were detected, suggesting a role for AcPIM6 in the structural
integrity of the cell wall and plasma membrane. These defects were corrected
by ectopic expression of the pimE gene. Metabolic pulse-chase radiolabeling
and cell-free PIM biosynthesis assays indicated that PimE catalyzes the (Sa(B1,2-mannosyl transfer for the AcPIM5 synthesis. Mutation
of an Asp residue in PimE that is conserved in and required for the activity
of human PIG-M resulted in loss of PIM-biosynthetic activity, indicating that
PimE is the catalytic component. Finally, PimE was localized to a distinct
membrane fraction enriched in AcPIM4-6 biosynthesis. Taken together, PimE
represents the first PPM-dependent mannosyl-transferase shown to be involved
in PIM biosynthesis, where it mediates the fifth mannose transfer.
Descriptors: Mycobacterium, phosphatidylinositol mannosides
(PIMs), cell wall and plasma membrane changes and organization, polyprenol-phosphate-mannose
(PPM).
Mustafa, A.S.; Skeiky, Y.A.; Al
Attiyah, R.; Alderson, M.R.; Hewinson, R.G.; Vordermeier, H.M. Immunogenicity of Mycobacterium tuberculosis antigens in
Mycobacterium bovis BCG-vaccinated and M. bovis-infected cattle.
Infection and immunity (IAI).
2006 Aug.; 74 (8): 4566-4572. ISSN: 0019-9567
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: The development of novel vaccine strategies supplementing
Mycobacterium bovis BCG (BCG) constitutes an urgent research challenge.
To identify potential subunit vaccine candidates, we have tested a series
of eight recently identified Mycobacterium tuberculosis antigens in
M. bovis-infected and BCG-vaccinated cattle. These antigens were characterized
on the basis of their ability to induce in vitro gamma interferon responses
in infected or BCG-vaccinated calves. We were able to establish a hierarchy
of these antigens based on how frequently they were recognized in both groups
of animals. In particular, we were able to prioritize frequently recognized
proteins like Rv0287, Rv1174, and Rv1196 for future evaluation as subunit
vaccines to be used in BCG-protein heterologous prime-boost vaccination scenarios.
In addition, the antigen most dominantly recognized in M. bovis-infected
cattle in this study, Rv3616c, was significantly less frequently recognized
by BCG vaccinees and could be a target to improve BCG, for example, by increasing
its secretion, in a recombinant BCG vaccine.
Descriptors: cattle, vaccines, subunit vaccine candidates, eight Mycobacterium
tuberculosis antigens, Mycobacterium bovis-infected and BCG-vaccinated
cattle, Rv3616c antigen.
Naranjo, V.; Ayoubi, P.; Vicente,
J.; Ruiz-Fons, F.; Gortazar, C.; Kocan, K.M.; De la Fuente, J. Characterization of selected genes upregulated in non-tuberculous
European wild boar as possible correlates of resistance to Mycobacterium
bovis infection. Veterinary Microbiology.
2006 Aug 25; 116 (1-3): 224-231. ISSN: 0378-1135
URL: http://dx.doi.org/10.1016/j.vetmic.2006.03.013
NAL Call Number: SF601.V44
Abstract: Bovine tuberculosis (bTB), caused by Mycobacterium bovis
(Mycobacterium tuberculosis complex), is a zoonotic disease that affects
cattle and wildlife worldwide. These animal hosts can serve as reservoirs
of infection, thus increasing the risk of human exposure and infection. In
this study we quantified by RNA macroarray fluorescent hybridization and real-time
RT-PCR the mRNA levels of genes differentially expressed in oropharyngeal
tonsils and mandibular lymph nodes of three and seven individual non-tuberculous
and tuberculous wild boars naturally exposed to M. bovis, respectively.
These results demonstrated upregulation of two genes, complement component
3 (C3) and methylmalonyl-CoA mutase (MUT), in the non-tuberculous wild boars.
These upregulated genes may contribute to resistance of wild boars to bTB
by modifying the innate immunity, which limits the ability of the mycobacterium
to infect and persist within macrophages. The C3 and MUT genes, therefore,
are likely to be good candidates to study as markers of bTB resistance using
functional genomics in animal model systems. Identification of genes upregulated
in wild animals resistant to bTB contributes to our understanding of the mechanisms
of protective immunity and resistance to mycobacterial organisms.
Descriptors: Mycobacterium bovis, wild boars, wildlife disease
reservoir, up regulated genes, resistant of boars to tuberculosis, limits
Mycobacterium to infect and persist in macrophages.
Parra, Marcela;
Cadieux, Nathalie; Pickett,
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: Infection of mice with Mycobacterium avium or immunization
with a novel PE gene expressed by M. avium (MaPE) showed that a dominant
T-cell immune response was elicited. Immunization with an MaPE DNA vaccine protected mice against an aerosol challenge
with Mycobacterium tuberculosis, suggesting that mycobacteria express
PE antigens with cross-protective T-cell epitopes.
Pereira-Suarez,
A.L.; Estrada-Chavez, C.; Arriaga-Diaz, C.; Espinosa-Cueto, P.; Mancilla,
R. Coexpression of NRAMP1, iNOS, and nitrotyrosine
in bovine tuberculosis. Veterinary Pathology.
2006; 43 (5): 709-717. ISSN: 0300-9858
URL: http://www.acvp.org
NAL Call Number: 41.8 P27
Abstract: In murine models the inducible nitric oxide synthase (iNOS)
and the natural resistance associated macrophage protein (NRAMP1) play major
roles in host defence against mycobacteria. iNOS regulates nitric oxide (NO) production, which is noxious
for ingested mycobacteria, and NRAMP1 displays pleiotropic antimicrobial effects,
including upregulation of iNOS expression. Little is known about the role
of these molecules in bovine tuberculosis (TB). In this work we demonstrate
by Western blot a high expression of NRAMP1 in peripheral blood mononuclear
cells (PBMCs), alveolar macrophages (obtained by bronchioalveolar lavage),
and lymph node granulomas from 8 Holstein-Freisian cattle with autopsy-proven
bovine TB. Immunohistochemistry revealed the abundant expression of NRAMP1
and iNOS in lymph node and lung granulomas. Immunoreactivity was abundant
in the cytoplasm of many epithelioid macrophages and multinucleated giant
cells of the Langhans type. A striking accumulation of nitrotyrosine (NT),
an indicator of iNOS activity and local NO production, was observed in granuloma
cells, particularly in multinucleated Langhans cells. This study shows that
the expression of NRAMP1 and iNOS is costimulated in granulomas, which are
protective T-cell reactions against mycobacteria..
Descriptors: Holstein-Freisian cattle, murine model, lymph node granulomas,
T cells, nitric oxide synthase; nitrotyrosine Mycobacterium bovis.
Pignone, Michelle;
Greth, Kimberly M.; Cooper, Jason; Emerson, David; Tang, Jane. Identification
of mycobacteria by matrix-assisted laser desorption ionization-time-of-flight
mass spectrometry. Journal of Clinical Microbiology.
2006 June; 44 (6): 1963-1970. ISSN: 0095-1137
URL: http://jcm.asm.org/cgi/content/full/44/6/1963
NAL Call Number: QR46 .J6
Abstract: Classical methods for identification of Mycobacterium
species rely on morphology and biochemical profiles. Speciation of a Mycobacterium
isolate using these standard methods is a lengthy process based on subjective
data interpretation. In this study, Mycobacterium species were characterized
by utilizing matrix-assisted laser desorption ionization-time-of-flight mass
spectrometry (MALDI-TOF MS). This technology is designed to provide a characteristic
mass spectral fingerprint based on desorbed ions from the cell surface. Thirty-seven
strains were analyzed; these represented thirteen species and five subspecies
that included the Mycobacterium tuberculosis complex and the M.
avium M. intracellulare complex, as well as rapid- and slow-growing
mycobacteria. All 37 strains were analyzed in triplicate, and a database
was generated. This method produced species-specific patterns for all but
1 of the 37 isolates and provided reliable differentiation at the strain level.
The data suggest that whole-cell MALDI-TOF MS has potential as a rapid and
reproducible method for the identification and characterization of Mycobacterium
species.
Descriptors: Mycobacterium species, speciation, characterized
by mass spectrometry method, spectral fingerprint.
Pollock, J.M.;
Rodgers, J.D.; Welsh, M.D.; McNair, J. Pathogenesis of bovine tuberculosis:
the role of experimental models of infection. Veterinary
Microbiology. 2006 Feb. 25; 112 (2-4) 141-150. ISSN: 0378-1135.
Note: Paper presented at the 4th International Conference on Mycobacterium
bovis, Held August 22-26, 2005,
URL:
http://www.elsevier.com/wps/find/journaldescription.cws_home/503320/description#description
NAL Call Number: SF601.V44
Abstract: In many countries, test-and-slaughter policies based on tuberculin
skin testing have made a significant impact on the control of bovine tuberculosis
(caused by infection with Mycobacterium bovis). However, in some countries
these policies have not proved as effective and improved disease control strategies
are required (including improved diagnostic tests and development of vaccines).
The host pathogen interactions in bovine tuberculosis are very complex. While
studies of the disease in naturally infected field cases of bovine tuberculosis
have provided valuable information, detailed knowledge can also be gained
through studies of disease models. A number of studies have developed M.
bovis infection models employing a range of routes and challenge doses.
An early objective was assessment of vaccine efficiency, and models of infection
remain central to current work in this area. Development
of the intra-nasal and intra-tracheal models have also advanced our
understanding of the kinetics of the immune response. In many of these studies,
understanding of pathogenesis has been improved by definition of the cells
that respond to infection and those that are instrumental in modulation of
host responses. Experimental models of infection have been adapted to study
cattle to cattle transmission, modeling one of the fundamental routes of infection.
This review provides a historical perspective on the types of experimental
models used in over 100 years of research and outlines new opportunities to
refine those methods for bovine and human tuberculosis and to contribute to
improved diagnostics, advanced understanding of immunology and vaccine design.
Descriptors: cattle, Mycobacterium bovis, animal pathogenic
bacteria, bovine tuberculosis, disease control, disease control programs,
literature reviews, animal disease models, infection, disease diagnosis, analytical
kits, vaccines, host pathogen relationships, pathogenesis, resistance mechanisms,
disease transmission, humans, tuberculosis.
Rasolofo-Razanamparany, V.; Quirin,
R.; Rapaoliarijaona, A.; Rakotoaritahina, H.; Vololonirina, E.J.; Rasolonava,
T.; Ferdinand, S.; Sola, C.; Rastogi, N.; Ramarokoto, H. Usefulness of
restriction fragment length polymorphism and spoligotyping for epidemiological
studies of Mycobacterium bovis in
URL: http://dx.doi.org/10.1016/j.vetmic.2005.11.057
NAL Call Number: SF601.V44
Abstract: Tuberculosis is highly prevalent in cattle in
Descriptors: zebu cattle, Mycobacterium bovis, bovine tuberculosis,
animal pathogenic bacteria, restriction fragment length polymorphism, epidemiology,
genotype, strains, strain differences, microbial genetics, disease transmission,
humans, zoonoses, goats, spoligotyping, Internet resource, Madagascar.
Razanamparany, V.R.; Quirin, R.;
Rapaoliarijaona, A.; Rakotoaritahina, H.; Vololonirina, E. J.; Rasolonavalona,
T; Ferdinand, S.; Sola, C.; Rastogi, N.; Ramarokoto, H.; Chanteau, S. Usefulness
of restriction fragment length polymorphism and spoligotyping for epidemiological
studies of Mycobacterium bovis in
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/503320/description#description
NAL Call Number: SF601.V44
Abstract: Tuberculosis is highly prevalent in cattle in
Descriptors: Zebu cattle, Mycobacterium bovis, disease prevalence,
disease transmission between animals and humans, epidemiology, spoligotyping,
genetic diversity, genetic markers, genetic polymorphism, genotypes, microsatellites,
zoonoses,
Ren, Huiping; Dover, Lynn G.;
Islam, Salim T.; Alexander, David C.; Chen, Jeffrey M.; Besra, Gurdyal S.;
Liu, Jun. Identification of the lipooligosaccharide biosynthetic
gene cluster from Mycobacterium marinum. Molecular Microbiology. 2007; 63 (5): 1345-1359. ISSN:
0950-382X
URL: http://www.blackwell-synergy.com/loi/mmi
Abstract: Lipooligosaccharides (LOSs) are antigenic glycolipids that
are present in some species of Mycobacterium including the Canetti
strain of M. tuberculosis. The core LOS structures from several mycobacterial
organisms have been established, but the biosynthetic pathways of LOSs remain
unknown. In this study, we describe two transposon insertion mutants of M.
marinum that exhibit altered colony morphology. Cell wall analysis reveals
that the MRS1271 mutant is defective in the synthesis of LOS-II, whereas the
MRS1178 mutant accumulates an intermediate between LOS-I and -II. The genetic
lesions were localized to two genes, MM2309 and MM2332. MM2309 encodes a
UDP-glucose dehydrogenase that is involved in the synthesis of D-xylose. MM2332
is predicted to encode a decarboxylase. These two genes and a previously
identified losA gene are localized in a gene cluster likely to be involved
in the biosynthesis of LOSs. Our results also show that LOSs play an important
role in sliding motility, biofilm formation, and infection of host macrophages.
Taken together, our studies have identified, for the first time, a LOS biosynthetic
locus. This is an important step in assessing the differential distribution
of LOSs among Mycobacterium species and understanding the role of LOSs
in mycobacterial virulence.
Descriptors: glycolipids, mutants, Mycobacterium marinum, lipooligosaccharides.
Richter, Elvira;
Reusch Gerdes, Sabine; Hillemann, Doris. Evaluation of the genotype Mycobacterium assay for identification
of mycobacterial species from cultures. Journal
of Clinical Microbiology. 2006 May; 44 (5): 1769-1775. ISSN:
0095-1137
NAL Call Number: QR46 .J6
Abstract: A new commercially available DNA strip assay (GenoType Mycobacterium
CM/AS; Hain Lifescience,
Descriptors: Mycobacterium species, 2 diagnostic test strips,
culture testing, soecies differentiation.
Robinson, Nirmal;
Wolke, Martina; Ernestus, Karen;
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: Virulent mycobacteria cause arrest of phagosome maturation
as a part of their survival strategy in hosts. This process is mediated through
multiple virulence factors, whose molecular nature remains elusive. Using
Mycobacterium marinum as a model, we performed a genome-wide screen
to identify mutants whose ability to inhibit phagosome maturation was impaired,
and we succeeded in isolating a comprehensive set of mutants that were not
able to occupy an early endosome-like phagosomal compartment in mammalian
macrophages. Categorizing and ordering the multiple mutations according to
their gene families demonstrated that the genes modulating the cell envelope
are the principal factors in arresting phagosome maturation. In particular,
we identified a novel gene, pmiA, which is capable of influencing the constitution
of the cell envelope lipids, thereby leading to the phagosome maturation block.
The pmiA mutant was not able to resist phagosome maturation and was severely
attenuated in mice. Complementing the mutant with the wild-type gene restored
the attenuated virulence to wild-type levels in mice.
Descriptors: Mycobacterium marinum, interference with phagosome
maturation, screening for mutants, multiple mutations found, novel gene pmiA,
affects cell envelope lipids, mouse model.
Romero, Beatriz; Aranaz, Alicia;
Juan, Lucaia de; a Alvarez, Julio; Bezos, Javier; Mateos, Ana; Gaomez-Mampaso,
Enrique; Domainguez, Lucas. Molecular epidemiology of multidrug-resistant
Mycobacterium bovis Isolates with the same spoligotyping profile as
isolates from animals. Journal of Clinical Microbiology
(JCM). 2006 Sept; 44 (9): 3405-3408. ISSN: 0095-1137
URL: http://jcm.asm.org/cgi/content/full/44/6/1963
Abstract: PCR-based characterization techniques have been adopted in
most laboratories for Mycobacterium bovis typing. We report a molecular characterization
of human multidrug-resistant M. bovis isolates and three bovine isolates
that share the spoligotyping profile. The analysis of the direct repeat region
showed that both groups differed in the presence of spacers not included in
the current membrane. They were also distinguished by two out of the nine
mycobacterial interspersed repetitive unit variable-number tandem repeat loci
tested, indicating that the human infection was not acquired from the cattle
from which isolates were obtained. These results highlight that a combination
of techniques is required for appropriate discrimination, even for those spoligotypes
that have a low frequency.
Descriptors: Mycobacterium bovis, molecular characterization
of human and bovine strains, spoligotyping profile, differences, interspersed
repetitive unit variable number tanden repeat loci, methods.
Ren, Huiping; Dover, Lynn G.;
Islam, Salim T.; Alexander, David C.; Chen, Jeffrey M.; Besra, Gurdyal S.;
Liu, Jun. Identification of the lipooligosaccharide
biosynthetic gene cluster from Mycobacterium marinum.
Molecular Microbiology. 2007; 63 (5): 1345-1359. ISSN:
0950-382X
URL: http://www.blackwell-synergy.com/loi/mmi
Abstract: Lipooligosaccharides (LOSs) are antigenic glycolipids that
are present in some species of Mycobacterium including the Canetti
strain of M. tuberculosis. The core LOS structures from several mycobacterial
organisms have been established, but the biosynthetic pathways of LOSs remain
unknown. In this study, we describe two transposon insertion mutants of M.
marinum that exhibit altered colony morphology. Cell wall analysis reveals
that the MRS1271 mutant is defective in the synthesis of LOS-II, whereas the
MRS1178 mutant accumulates an intermediate between LOS-I and -II. The genetic
lesions were localized to two genes, MM2309 and MM2332. MM2309 encodes a
UDP-glucose dehydrogenase that is involved in the synthesis of D-xylose. MM2332
is predicted to encode a decarboxylase. These two genes and a previously
identified losA gene are localized in a gene cluster likely to be involved
in the biosynthesis of LOSs. Our results also show that LOSs play an important
role in sliding motility, biofilm formation, and infection of host macrophages.
Taken together, our studies have identified, for the first time, a LOS biosynthetic
locus. This is an important step in assessing the differential distribution
of LOSs among Mycobacterium species and understanding the role of LOSs
in mycobacterial virulence.
Descriptors: glycolipids, mutants, Mycobacterium marinum, lipooligosaccharides.
Rosenthal, K.L. Microbiology:
revisiting the gram stain and culture. Small Animal and Exotics Proceedings
of the North American Veterinary Conference, Volume 20, Orlando, Florida,
USA, 7-11 January, 2006. 2006: 1575-1577
URL: http://www.tnavc.org
Descriptors: pet birds, bacterial infections, Enterococcus,
Escherichia coli, Klebsiella, Mycobacterium, Mycoplasma,
Proteus, Pseudomonas, Staphylococcus aureus, Chlamydophila,
Gram stain, culture methods.
Rothschild,
B.M.; Martin, L.D. Did ice-age bovids spread tuberculosis?
Naturwissenschaften. 2006; 93 (11): 565-569. ISSN:
0028-1042
URL: http://www.springerlink.com/link.asp?id=100479
Abstract: Postcranial artiodactyl, perissodactyl, and carnivore skeletons
were examined in major university and museum collections of North America
and
Descriptors: paleontology, Mycobacterium bovis, paleozoology,
bone destruction and lesions; skeletons of artiodactyls, perissodactyls, and
carnivores, fossil bones, museum specimens, prehistoric vectors of bovine
Mycobacterium, North America; Europe.
Rothschild,
B.M.; Laub, R. Hyperdisease in the late Pleistocene: validation
of an early 20th century hypothesis. Naturwissenschaften.
2006; 93 (11): 557-564. ISSN: 0028-1042
URL: http://www.springerlink.com/link.asp?id=100479
Abstract: The hypothesis of disease-related large mammal extinction
has new support. A unique pathologic zone of resorption in 52% of metacarpels
and metatarsals was first noticed in a 113 skeletons of Hiscock Mammut
americanum metacarpals. There was also associated rib periosteal reaction
that is suggestive of tuberculosis. Foot lesions were identical to that documented
in Bison as pathognomonic for tuberculosis. The high frequency of
the pathology in M. americanum suggests that tuberculosis was pandemic,
a hyperdisease. Such pandemic tuberculosis could have been one of several
factors contributing to mastodon extinction.
Descriptors: paleozoology, fossils, mammals, Mycobacterium tuberculosis,
bacterial infections in feet bones, bacterioses, bone destruction, Mammut
americanum, Pleistocene era.
Rosseels, Valerie; Marche, Sylvie;
Roupie,Virginie; Govaerts, Marc; Godfroid, Jacques; Walravens, Karl; Huygen,
Kris. Members of the 30- to 32-kilodalton mycolyl transferase family (Ag85)
from culture filtrate of Mycobacterium avium subsp. paratuberculosis
are immunodominant Th1-type antigens recognized early upon infection in mice
and cattle. Infection and Immunity. 2006
Jan; 74 (1): 202-212. ISSN: 0019-9567
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: The characterization of protective antigens is essential
for the development of an effective, subunit-based vaccine against paratuberculosis.
Surface-exposed and secreted antigens, present abundantly in mycobacterial
culture filtrate (CF), are among the well-known protective antigens of Mycobacterium
tuberculosis and Mycobacterium bovis. Culture filtrate, prepared
from Mycobacterium avium subsp. paratuberculosis ATCC 19698
grown as a surface pellicle on synthetic Sauton medium, was strongly and early
recognized in experimentally infected B6 bg/bg beige mice and cattle, as indicated
by elevated spleen cell gamma interferon (IFN-[gamma]) secretion and lympho-proliferative
responses of peripheral blood mononuclear cells, respectively. Strong proliferative
and ex vivo IFN-[gamma] responses against antigen 85 (Ag85) complex
(a major protein component from M. bovis BCG culture filtrate) could
be detected in cattle as early as 10 weeks after oral M. avium subsp.
paratuberculosis infection. Synthetic peptides from the Ag85A and
Ag85B components of this complex were strongly recognized, whereas T-cell
responses were weaker against peptides from the Ag85C protein. A promiscuous
T-cell epitope spanning amino acids 145 to 162 of Ag85B (identical sequence
in M. bovis and M. avium subsp. paratuberculosis) was
identified in experimentally infected cattle. Finally, young calves, born
from cows with confirmed paratuberculosis, demonstrated proliferative responses
to purified, recombinant Ag85A and Ag85B from M. avium subsp. paratuberculosis.
These results indicate that the M. avium subsp. paratuberculosis
Ag85 homologues are immunodominant T-cell antigens that are recognized early
in experimental and natural infection of cattle.
Descriptors: cattle, Mycobacterium avium paratuberculosis,
Mycobacterium bovis, protective antigens, synthetic Sauton medium, cultural
filtrate, experimentally infections, B6 bg/bg beige mice. cattle,
elevated spleen cell gamma interferon (IFN-[gamma]) secretion, lympho-proliferative
responses of peripheral blood mononuclear cells, Ag85 homologues.
Rybniker, Jan;
Kramme, Stefanie; Small, Pamela L. Host range of 14 mycobacteriophages
in Mycobacterium ulcerans and seven other mycobacteria including Mycobacterium
tuberculosis - application for identification and susceptibility testing.
Journal of Medical Microbiology. 2006; 55(1):
37-42. ISSN: 0022-2615
URL: http://jmm.sgmjournals.org/contents-by-date.0.shtml
NAL Call Number: QR1.J62
Descriptors: Mycobacterium bovis strain BCG Pasteur; Mycobacterium
ulcerans strain M18, strain-RifR, strain, clinical-isolates, strain 1615
mycolactone--mutant, strain-1615 (ATCC-35840), strain S12; Mycobacterium
tuberculosis strain H37Rv, strain-371, strain BCG-Pasteur; Mycobacterium
avium strain 702, strain 701, strain 3746-02; Mycobacterium marinum
strain 565, strain ATCC-927; Mycobacterium scrofulaceum strain 1315,
strain 1320; Mycobacterium fortuitum strain 1529; Mycobacterium
chelonae strain 1543; Mycobacterium smegmatis strain mc-2-155;
mycobacteriophage strain TM4, strain D29, phage therapy.
Santillan-Flores,
M.A.; Flores, J.; Arriaga-Diaz, C.; Romero-Torres, C.; Suarez-Guemes, F.;
Espitia, C. Polymorphism of the PE domain of PE/PE_PGRS sequences
in clinical isolates of Mycobacterium bovis in
URL: http://dx.doi.org/10.1016/j.vetmic.2006.02.021
URL: http://www.sciencedirect.com/science/journal/03781135
NAL Call Number: SF601.V44
Abstract: Polymorphism of the PE domain of PE/PE_PGRS sequences
was studied in Mycobacterium bovis isolates from different Mexican
states. Samples were analyzed by spolygotyping and RFLP using IS6110 and
a 235-bp fragment of the PE domain of PE/PE_PGRS as probes. With the PE probe,
three different genotypes were observed, one being predominant in all states.
These results confirm the high conservation of the PE domain
and suggests a potential role for PE sequence as a stable genetic marker
for bovine tuberculosis.
Descriptors: Mycobacterium bovis, RFLP, microbial genetics,
bacterial proteins, mycobacterial diseases, multigene family, genes, genotype,
genetic variation, genetic markers, nucleotide sequences, amino acid sequences,
spolygotyping, molecular sequence data,
Savelkoul, Paul H.M.; Catsburg,
Arnold; Mulder, Sije; Oostendorp, Ludo; Schirm, Jurjen; Wilke, Hans; van der
Zanden, Adri G.M.; Noordhoek, Gerda T. Detection of Mycobacterium tuberculosis
complex with Real Time PCR: comparison of different primer-probe sets based
on the IS6110 element. Journal of Microbiological
Methods. 2006; 66 (1): 177-180. ISSN: 0167-7012
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/506034/description#description
NAL Call Number: QR65.J68
Descriptors: diagnostic testing, sensitivity and specificity, real
time PCR: real-time-polymerase-chain-reaction, laboratory echniques, genetic
techniques, TaqMan, laboratory equipment, express software package, minor
groove binding probe, four real time PCR prime probe sets, Mycobacterium
tuberculosis, Mycobacterium avium, Mycobacterium intracellulare,
Mycobacterium kansasii, Mycobacterium fortuitum Mycobacterium
bovis, Mycobacterium gordonae, Mycobacterium xenopi, Mycobacterium
smegmatis strain 1008, Mycobacterium cordense, Mycobacterium
senegalanse, Nocardia farcinica.
Shah, N.P..; Singhal, A.; Jain,
A.; Kumar, P.; Uppal, S.S.; Srivatsava, M.V.P.; Prasad, H.K. Occurrence
of overlooked zoonotic tuberculosis: detection of Mycobacterium bovis
in human cerebrospinal fluid. Journal of Clinical
Microbiology. 2006 Apr; 44 (4) 1352-1358. ISSN: 0095-1137
URL: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1489394
NAL Call Number: QR46.J6
Abstract: The paucibacillary nature of the cerebrospinal fluid (CSF)
has been a major obstacle in the diagnosis of human tuberculous meningitis
(TBM). This study shows that with molecular techniques direct precise determination
to the species level of mycobacterial pathogens can be made. The present
report describes the utility of a nested PCR (N-PCR) assay (A. Mishra, A.
Singhal, D. S. Chauhan, V. M. Katoch, K. Srivastava, S. S. Thakral, S. S.
Bharadwaj, V. Sreenivas, and H. K. Prasad, J. Clin. Microbiol.
43:5670-5678, 2005) in detecting M. tuberculosis
and M. bovis in human CSF. In 2.8% (6/212) of the samples,
M. tuberculosis was detected, and in 17% (36/212), M. bovis was
detected. Mixed infection was observed in 22 samples. Comparative analysis
of clinical diagnosis, smear microscopy, and N-PCR in 69 patients (TBM, 25;
non-TBM, 44) showed that the sensitivity of N-PCR (61.5%) was greater than
that of smear microscopy (38.4%). Determination to the species level is important
from the viewpoint of determining the prevalence of these mycobacteria in
a community and would influence strategies currently adopted for the prevention
of tuberculosis.
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis,
differential diagnostic techniques, zoonotic diseases, epidemiology, prevalence,
disease prevention, nested PCR assay, utility of assay.
Slinina, K.N.;
Lazovskaya, A.L. Vorob' eva, Z.G.; Kul' chitskaya, M.A.; Druchkova, M.V.
A method for storage of cultures in the laboratory.
Russian Agricultural Sciences. 2006; (12):
24-25. ISSN: 1068-3674. Note: Translated journal.
NAL Call Number: S1.S68
Descriptors: storage methods, bacterial species, Escherichia coli,
Enterococcus faecalis, Mycobacterium smegmatis, Mycobacterium
avium strains, preservation of biochemical characteristics and properties.
Smith, N.H.;
Gordon, S.V.; Rua-Domenech, R. de la; Clifton-Hadley, R.S.; Hewinson, R.G.
Bottlenecks and broomsticks: the molecular evolution of Mycobacterium
bovis. Nature Reviews Microbiology.
2006; 4(9): 670-681. ISSN: 1740-1526
URL: http://www.nature.com/reviews
Descriptors: Mycobacterium bovis, cattle tuberculosis, reduction
in diversity, population bottlenecks, selective sweeps, shaping of phylogeny,
British Isles populations, spread of infection, improved vaccines, diagnostic,
tests, UK.
Teixeira, Francisco M.; Teixeira,
Henrique C.; Ferreira, Ana Paula; Rodrigues, Michele F.; Azevedo, Vasco; Macedo,
Gilson C.; Oliveira, Sergio C. DNA vaccine using Mycobacterium bovis
Ag85B antigen induces partial protection against experimental infection in
BALB/c mice. Clinical and Vaccine Immunology. 2006; 13(8): 930-935.
ISSN: 1556-6811
URL: http://cvi.asm.org/
NAL Call Number: RB 46.5
Descriptors: bovine tuberculosis, Mycobacterium bovis, mouse
model, Ag85B gene as a DNA vaccine, challenge with Mybacterium bovis
virulent strain (ATCC 19274), induction a Th1 type
of immune response, spleens, lungs.
Thoen, C.; LoBue,
P.; Kantor,
URL: www.sciencedirect.com/science/journal/03781135
NAL Call Number: SF601.V44
Abstract: Mycobacterium bovis and closely associated acid-fast
bacilli cause disease in humans. Epidemiologic investigations reveal that
the organism may be ingested or inhaled. Extra pulmonary lesions may occur
associated to the consumption of infected milk, even though with the practice
of boiling milk, and the growth of milk pasteurization plants all over the
world, the digestive route of infection became less important. On the other
hand, airborne infection continues to occur among meat industry and slaughterhouse
workers, in regions where the infection is still prevalent in cattle. Evidence
of person to person transmission is rare. Main causes of concern related
to M. bovis in industrialized countries are: epizootics in domesticated
and wild mammals and latent infection in immigrants. Although multi-drug-resistant
(MDR) strains of M. bovis have been identified, case reports reveal
that anti-tuberculosis drugs routinely used to treat Mycobacterium tuberculosis-infected
patients are effective when properly administered.
Descriptors: cattle, food animals, Mycobacterium bovis, animal
pathogenic bacteria, bovine tuberculosis, literature reviews, zoonoses, humans,
tuberculosis, disease transmission, lesions animal, health hazards, occupational
health and safety, livestock and meat industry, slaughterhouses, disease outbreaks,
wild animals, latent period, multiple drug resistance, asymptomatic infections.
Tobler, Nadia E.; Pfunder, Monika;
Herzog, Katrin; Frey, Juerg E.; Altwegg, Martin. Rapid detection and species identification of Mycobacterium
spp. using real-time PCR and DNA-Microarray. Journal
of Microbiological Methods. 2006; 66 (1): 116-124. ISSN: 0167-7012.
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/506034/description#description
NAL Call Number: QR65.J68
Descriptors: Mycobacterium, 37 different species, identification
procedure, 5' exonuclease real-time PCR, DNA microarray based on the region
upstream of 65 kDa heat shock protein, may be good for mixed infections as
well, Mycobacterium leprae, Mycobacterium abscessus, Mycobacterium
bovis, Mycobacterium intracellulare, Mycobacterium fortuitum,
Mycobacterium haemophilum, Mycobacterium lentiflavum, Mycobacterium
chelonae, Mycobacterium gordonae, Mycobacterium africanum,
Mycobacterium avium ssp paratuberculosis, Mycobacterium malmoense,
Mycobacterium avium avium, Mycobacterium genavense, Mycobacterium
celatum, Mycobacterium canettii, Mycobacterium alvei, Mycobacterium
heckenshornense, Mycobacterium heidelbergense.
Ung, Korine
S E; Av Gay, Yossef. Mycothiol-dependent mycobacterial
response to oxidative stress. FEBS
Letters. 2006; 580 (11): 2712-2716. ISSN: 0014-5793
URL:
http://www.elsevier.com/wps/find/journaldescription.cws_home/506085/description#description
NAL Call Number: QD415.F4
Descriptors: mycobacteria, Mycobacterium bovis BCG, Mycobacterium
smegmatis, esogenous oxidative stress, MSH levels, thiol-specific oxidant
diamide, hydrogen peroxide.
Viana-Niero, Cristina; Rosales-Rodriguez,
Cesar Alejandro; Bigi, Fabiana; Santos-Zanini, Marcos; Ferreira-Neto, Jose
Soares; Cataldi, Angel; Leao, Sylvia Cardoso. Identification of an IS6110 insertion site in plcD, the unique
phospholipase C gene of Mycobacterium bovis. Journal
of Medical Microbiology. 2006; 55 (4): 451-457. ISSN: 0022-2615
URL: http://jmm.sgmjournals.org/contents-by-date.0.shtml
NAL Call Number: QR1.J62
Descriptors: phospholipase C genes, plcA, plcB, plcC, plcD genes,
IS6110 single copy, IS6110 transposion, PCR, Southern blot hybridization and
sequencing analysis, Mycobacterium tuberculosis complex, Pvull
fragment, Mycobacterium bovis, Mycobacterium africanum,
Mycobacterium canettii, Mycobacterium microti.
Vitale, Fabrizio; Reale, Stefano;
Petrotta, Enrico; Caracappa, Santo; Barera, Annalisa; La Manna, Marco Pio;
Macaluso, Pasquale; Caccamo, Nadia; Dieli, Francesco; Vordermeier, Hans Martin;
Sireci, Guido; Salerno, Alfredo. ESAT-6 peptide recognition by bovine
CD8(+) lymphocytes of naturally infected cows in
herds from southern Italy. Clinical and Vaccine Immunology. 2006; 13 (4): 530-533.
ISSN:
URL: http://cvi.asm.org/
NAL Call Number: RB46.5
Descriptors: define epitopes of Mycobacterium bovis from ESAT-6
(early secretory antigen of 6 kDa) recognized by CD8(+)
T lymphocytes from cows naturally infected with Mycobacterium bovis,
bovine CD8' T cells recognized 10 out of 11 ESAT-6 peptides tested.
Waddington, K. The Bovine
Scourge: Meat, Tuberculosis and Public Health, 1850-1914. Boydell
Press. Suffock, UK2006; i-ix + 226 pp. ISBN: 1843831937.
Note: A book with 10 chapters on the topic of meat and TB.
NAL Call Number: SF967.T8 W33 2006
Descriptors: bovine tuberculosis, public health, food safety concerns,
meat form infected cattle, transmissibility between species and humans, meat
inspection, eradication, etc.
Walravens, K.; Allix, C.; Supply,
P.; Rigouts, L.; Godfroid, J.; Govaerts, M.; Portaels, F.; Dufey, J.; Vanholme,
L.; Fauville-Dufaux, M.; Saegerman, C. Apports du genotypage des souches
de Mycobacterium bovis a l'analyse de l'epidemiologie
de la tuberculose bovine en Belgique (1995-2005). [Genotyping
of the strains of Mycobacterium bovis isolated in
Descriptors: cattle, Mycobacterium bovis pathogen, molecular
typing of isolates, strains, restriction techniques, fragment length polymorphism
(RFLP IS6110) and spoligotyping, mycobacterial interspersed repetitive units,
variable number tandem repeat analysis (MIRU-VNTR), 40 genotypes, 12 lineages,
epidemiology, Belgium.
Wedlock, D.N.; Kawakami, R.P.;
Koach, J.; Buddle, B.M.; Collins, D.M. Differences of gene expression in
bovine alveolar macrophages infected with virulent and attenuated isogenic
strains of Mycobacterium bovis. International Immunopharmacology. 2006; 6 (6): 957-961.
ISSN: 1567-5769.
URL:
http://www.elsevier.com/wps/find/journaldescription.cws_home/621330/description#description
NAL Call Number: QR180.I52
Descriptors: DNA microarray analysis to detect genes expressed in
infected bovine lung alveolar macrophages, two isogenic strains of M bovis,
virulent strain ATCC35723, attenuated strain WAg520 derived from ATCC35723,
chemokines, interleukin 8, monocyte chemotactic protein 1, identification
of key genes, early and protective immune responses to tuberculosis.
2005
Amadio, Ariel; Romano, Maria-Isabel;
Bigi, Fabiana; Etchechoury, Ignacio; Kubica, Tanja; Niemann, Stefan; Cataldi,
Angel; Caimi, Karina. Identification and characterization
of genomic variations between Mycobacterium bovis and M. tuberculosis
H37Rv. Journal of Clinical Microbiology. 2005; 43 (5): 2481-2484.
ISSN: 0095-1137
URL: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1489394
NAL Call Number: QR46.J6
Abstract: Genetic differences between Mycobacterium bovis and
M. tuberculosis were identified. We found (i) a deletion of Rv3479
specific to M. bovis, (ii) that the rpfA gene is shortened to various
extents in M. bovis, and (iii) an insertion in Rv0648 and a duplication
of lppA common in M. tuberculosis complex isolates.
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis,
genetic differences, gene deletions, gene shortenings, insertion gene, duplication
of common M. tuberculosis complex.
Amadio, A.; Romano, M.I.; Bigi,
F.; Etchechoury, I.; Kubica, T.; Niemann, S.; Cataldi, A.; Caimi, K. Identification and characterization of genomic variations between
Mycobacterium bovis and M. tuberculosis H37Rv. Journal
of Clinical Microbiology. 2005; 43 (5): 2481-2484. ISSN: 0095-1137
URL: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1489394
NAL Call Number: QR46.J6
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis,
genetic analysis, transposable elements, genetic deletions, deletion of Rv3479
specific to M. bovis, rpfA gene is shortened to various extents in
Mycobacterium bovis, insertion in Rv0648 and a duplication of lppA
common in Mycobacterium tuberculosis complex isolates.
Bakshi, C.S.;
Shah, D.H.; Verma, Rishendra; Singh, R.K; Malik, Meenakshi. Rapid
differentiation of Mycobacterium bovis and Mycobacterium tuberculosis
based on a 12.7-kb fragment by a single tube multiplex-PCR. Veterinary Microbiology. 2005; 109 (3-4): 211-216.
ISSN: 0378-1135
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/503320/description#description
NAL Call Number: SF601.V44
Abstract: The aim of this work was the design and validation of a rapid
and easy single tube multiplex-PCR (m-PCR) assay for the unequivocal differential
detection of Mycobacterium bovis and Mycobacterium tuberculosis.
Oligonucleotide primers were based on the uninterrupted 229-bp sequence in
the M. bovis genome and a unique 12.7-kb insertion sequence from the
M. tuberculosis genome, which is responsible for species-specific genomic
polymorphism between these two closely related pathogens. The m-PCR assay
was optimized and validated using 22 M. bovis and 36 M. tuberculosis
clinical strains isolated from diverse host species and 9 other non-tuberculous
mycobacterial (NTM) strains. The designed primers invariably amplified a
unique 168-bp (M. bovis-specific) and 337-bp (M. tuberculosis-specific)
amplicon from M. bovis and M. tuberculosis strains, respectively.
The accuracy of the assay, in terms of specificity, was 100%, as none of
the NTM strains tested revealed any amplification product. As little as 20
pg of genomic DNA could be detected, justifying the sensitivity of the method.
The m-PCR assay is an extremely useful, simple, reliable and rapid method
for routine differential identification of cultures of M. bovis and
M. tuberculosis. This m-PCR may be a valuable diagnostic tool in areas
of endemicity, where bovine and human tuberculosis coexist, and the distinction
of M. bovis from M. tuberculosis is required for monitoring
the spread of M. bovis to humans.
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis,
differential diagnosis, PCR assay technique.
Biet, Franck;
Boschiroli, Maria Laura; Thorel, Marie Francoise; Guilloteau, Laurence A.
Zoonotic aspects of Mycobacterium bovis and Mycobacterium
avium-intracellulare complex (MAC). Veterinary
Research (Les Ulis). 2005; 36(3): 411-436. ISSN: 0928-4249.
URL: http://www.edpsciences.org/journal/index.cfm?edpsname=vetres
NAL Call Number: SF602.A5
Descriptors: Mycobacterium avium, Mycobacterium bovis,
Mycobacterium avium ssp. avium, Mycobacterium intracellulare
complex, epidemiology, zoonotic diseases, transmission between environment
and wildlife, etiology, possibilities of control and management, Europe, North
America, New Zealand.
Bigi, Fabiana; Garcia-Pelayo,
M. Carmen; Nunez-Garcia, Javier; Peralta, Andrea; Caimi, Karina C.; Golby,
Paul; Hinds, Jason; Cataldi, Angel; Gordon, Stephen V.; Romano, Maria I. Identification of genetic markers for Mycobacterium pinnipedii
through genome analysis. FEMS Microbiology
Letters. 2005; 248 (2): 147-152. ISSN: 0378-1097
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/506058/description#description
NAL Call Number: QR1.F44
Descriptors: seals, Mycobacterium pinnipedii, genetic variability
with Mycobacterium bovis, microarray-based comparative genomics, 2
deletions identified, M. tuberculosis genes, PiD1--Rv3530c and
Rv3531c, PiD2--Rv1977 and Rv1978.
Bigi, F.; Gioffre, A.; Klepp,
L.; Santangelo, M.P.; Velicovsky, C.A.; Giambartolomei, G.H.; Fossati, C.A.;
Romano, M.I.; Mendum, T.; McFadden, J.J.; Cataldi, A. Mutation in the
P36 gene of Mycobacterium bovis provokes attenuation of the bacillus
in a mouse model. Tuberculosis (
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/638428/description?navopenmenu=-2
Descriptors: Mycobacterium tuberculosis, Mycobacterium bovis,
P36 proteins, putative virulence factor of wild type pathogen, mouse model.
Burguiere, Adeline; Hitchen, Paul
G; Dover, Lynn G; Kremer, Laurent; Ridell, Malin; Alexander, David C.; Liu,
Jun; Morris, Howard R.; Minnikin, David E.; Dell, Anne; Besra, Gurdyal S.
LosA, a key glycosyltransferase involved in the biosynthesis of a novel family
of glycosylated acyltrehalose lipooligosaccharides from Mycobacterium marinum.
Journal of Biological Chemistry. 2005 Dec 23;
280 (51): 42124-42133. ISSN: 0021-9258
URL: http://www.jbc.org/
NAL Call Number: 381 J824
Abstract: Members of the genus Mycobacterium are characterized
by cell envelopes rich in unusual free lipids, interacting with a covalently
anchored mycolyl-arabinogalactan matrix. Previous studies have shown that
Mycobacterium marinum produces large amounts of a diacylglycosylphenolphthiocerol,
"phenolic" glycolipid. When cultivated on liquid Sauton medium,
traces of a polar lipooligosaccharide (LOS) glycolipid antigen were also previously
indicated. In this study, it was found that growth of the type strain of
M. marinum on solid Sauton or Middlebrook 7H10 agar gave substantial,
but different, amounts of a family of four major trehalose-based LOSs. The
core pentasaccharide LOS-I was a rhamnosyl diglucosyl-acylated trehalose.
The heptasaccharide, LOS-II, was derived from LOS-I by adding xylose accompanied
by a novel sugar (X); repeated addition of this sugar unit X gave the octasaccharide
LOS-III. LOS-IV has a decasaccharide component with two additional unusual
sugar units, YZ. In a recent study (Alexander, D. C., Jones, J. R., Tan,
T., Chen, J. M., and Liu, J. (2004) J. Biol. Chem. 279, 18824-18833),
chromatographically similar glycolipids were assigned to the family of phosphatidylinositol
mannosides (PIMs) and a "PimF" (Rv1500) glycosyltransferase implicated
in the conversion of a supposed "PIM subscript 5" to a "PIM
subscript 7." The present study indicates that these putative PIMs are
in fact members of the phosphorus-free LOS family of glycolipids and that
the protein product of Rv1500, which we have now termed LosA, is a glycosyltransferase
involved in transferring sugars to LOS-III to form LOS-IV of M. marinum.
Descriptors: Mycobacterium marinum, cultured on solid Sauton
medium, liquid Sauton medium, lipids, mycolyl-arabinogalactan matrix, putative
PIMs.
Clifton-Hadley, R.S.; Inwald,
J.; Archer, J.; Hughes, S.; Palmer, N.; Sayers, A.R.; Sweeney, K.; Embden,
J.D.A. van; Hewinson, R.G. Recent advances in DNA fingerprinting using
spoligotyping - epidemiological applications in bovine TB. Cattle Practice. 2005; 13 (4): 347-350. ISSN: 0969-1251
NAL Call Number: SF961.C37
Descriptors: cattle, badgers (Meles meles), Mycobacterium
bovis strains, molecular fingerprinting techniques, uses in epidemiology,
Cobos-Marin,
L.; Montes-Vargas, J.; Zumarraga, M.; Cataldi, A.; Romano, M.I.; Estrada-Garcia,
NAL Call Number: 448.8 C162
Descriptors: cattle, Mycobacterium bovis, animal pathogenic
bacteria, strains, bovine tuberculosis, genetic techniques and protocols,
polymerase chain reaction, loci, repetitive sequences, spoligotyping, spacer
oligonucleotide typing, spoligotypes, Mexico.
Collins, Desmond
A.; Skou, Bronwyn; White, Stefan; Bassett, Shalome; Collins, Lauren; For,
Raewyn; Hurr, Kathryn; Hotter, Grant; de Lisle, Geoffrey W. Generation
of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis
for discovery of novel vaccine candidates. Infection
and Immunity. 2005; 73 (4): 2379-2386. ISSN: 0019-9567.
URL: http://iai.asm.org
NAL Call Number: QR1.I57
Abstract: Mycobacterium bovis, a member of the Mycobacterium
tuberculosis complex, has a particularly wide host range and causes tuberculosis
in most mammals, including humans. A signature tag mutagenesis approach,
which employed illegitimate recombination and infection of guinea pigs, was
applied to M. bovis to discover genes important for virulence and to
find potential vaccine candidates. Fifteen attenuated mutants were identified,
four of which produced no lesions when inoculated separately into guinea pigs.
One of these four mutants had nine deleted genes including mmpL4 and sigK
and, in guinea pigs with aerosol challenge, provided protection against tuberculosis
at least equal to that of M. bovis BCG. Seven mutants had mutations
near the esx4 (esat-6) locus, and immunoblot analysis of these confirmed the
essential role of other genes at this locus in the secretion of EsxA (ESAT-6)
and EsxB (CFP10). Mutations in the eight other attenuated mutants were widely
spread through the chromosome and included pks1, which is naturally inactivated
in clinical strains of M. tuberculosis. Many genes identified were
different from those found by signature tag mutagenesis of M. tuberculosis
by use of a mouse infection model and illustrate how the use of different
approaches enables identification of a wider range of attenuating mutants.
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis complex,
genes important for virulence, potential vaccine candidates, attenuated mutants,
guinea pig animal model.
de Jong, Bouke C.; Onipede, Anthony;
Pym, Alex S.; Gagneux,-Sebastien; Aga, Roxanne S.; DeRiemer, Kathryn; Small,
Peter M. Does resistance to pyrazinamide accurately indicate the presence
of Mycobacterium bovis. Journal
of Clinical Microbiology. 2005; 43 (7) 3530-3532. ISSN: 0095-1137
URL: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1489394
NAL Call Number: QR46.J6
Abstract: Mycobacterium bovis is best identified by screening
those isolates of the Mycobacterium tuberculosis complex that have
any pyrazinamide (PZA) resistance, using a confirmatory test such as spoligotyping,
biochemical testing, or genomic deletion analysis. The sensitivity for detection
of M. bovis is lowered to 82% when only PZA-monoresistant isolates
are screened.
Descriptors: Mycobacterium bovis,
isolate screening, pyrazinamide resistance, spoligotyping, biochemical testing,
genomic deletion analysis.
Denis, M.; Buddle,
B.M. Iron modulates the replication of virulent Mycobacterium
bovis in resting and activated bovine and possum macrophages. Veterinary
Immunology and Immunopathology. 2005; 107 (3-4) 189-199. ISSN:
0165-2427
URL: http://www.elsevier.com/wps/find/journalabstracting.cws_home/503319/abstracting
NAL Call Number: SF757.2.V38
Descriptors: Mycobacterium bovis, bacterial replication,
in vitro testing, resting and activated possum macrophages, resting and
activated bovine macrophages, iron modulating effects, virulent strains.
Denis, Michel;
Wedlock, D. Neil; Buddle, Bryce M. IFN-gamma enhances bovine macrophage
responsiveness to Mycobacterium bovis: Impact on bacterial replication,
cytokine release and macrophage apoptosis. Immunology
and Cell Biology. 2005; 83 (6): 643-650. ISSN: 0818-9641
URL: http://www.blackwell-synergy.com/loi/icb
NAL Call Number: QR180.I43
Descriptors: Mycobacterium bovis, bovine macrophages, effect
of IFN-gamma, NO levels, IL-12, pro-inflammatory mediators, BCG infection,
combination of IFN and LPS caused reduction of bacterial replication, apoptotic
pathway, TNF alpha release.
Fujita, Yukiko;
Naka, Takashi; Doi, Takeshi; Yano, Ikuya. Direct molecular mass determination of trehalose monomycolate from
11 species of mycobacteria by MALDI-TOF mass spectrometry.
Microbiology (Reading). 2005; 151(Part
5): 1443-1452. ISSN: 1350-0872.
URL: http://mic.sgmjournals.org/contents-by-date.0.shtml
NAL Call Number: QR1.J65
Descriptors: mycobacteria, molecular mass of cell wall component,
matrix assisted laser desorption/ionization time-of-flight mass spectrometry,
numbers of carbons and double bonds, mycolic acid, species differences, Mycobacterium
tuberculosis, Mycobacterium bovis wild and BCG strains, Mycobacterium
avium intracellulare group, Mycobacterium phlei, Mycobacterium
flavescens, procedure does not require degradation process.
Fujita, Y.;
Naka, T.; McNeil, M.R.; Yano, I. Intact molecular characterization
of cord factor (trehalose 6,6'-dimycolate) from nine
species of mycobacteria by MALDI-TOF mass spectrometry. Microbiology
(Reading). 2005; 151 (10): 3403-3416. ISSN: 1350-0872
URL: http://mic.sgmjournals.org/contents-by-date.0.shtml
NAL Call Number: QR1.J64
Descriptors: Mycobacterium avium, Mycobacterium bovis,
Mycobacterium kansasii, Mycobacterium phlei, Mycobacterium
tuberculosis, Mycobacterium flavescens, species differentiation,
species characterization,molecular genetics, ions, molecular conformation,
physicochemical properties, trehalose.
Gibson, Andrea;
Brown, Timothy; Baker, Lucy; Drobniewski, Francis. Can 15 locus
mycobacterial interspersed repetitive unit-variable-number tandem repeat analysis
provide insight into the evolution of Mycobacterium tuberculosis?
Applied and Environmental Microbiology. 2005
Dec.; 71 (12): 8207-8213. ISSN: 0099-2240
URL: http://aem.asm.org/contents-by-date.0.shtml
NAL Call Number: 448.3 Ap5
Abstract: The phylogeny and evolution of the bacterium Mycobacterium
tuberculosis is still poorly understood despite the application of a variety
of molecular techniques. We analyzed 469 M. tuberculosis and 49 Mycobacterium
bovis isolates to evaluate if the mycobacterial interspersed repetitive
units-variable-number tandem repeats (MIRU-VNTR) commonly used for epidemiological
studies can define the phylogeny of the M. tuberculosis complex. This
population was characterized by previously identified silent single-nucleotide
polymorphisms (sSNPs) or by a macroarray based on these sSNPs that was developed
in this study. MIRU-VNTR phylogenetic codes capable of differentiating between
phylogenetic lineages were identified. Overall, there was 90.9% concordance
between the lineages of isolates as defined by the MIRU-VNTR and sSNP analyses.
The MIRU-VNTR phylogenetic code was unique to M. bovis and was not
observed in any M. tuberculosis isolates. The codes were able to differentiate
between different M. tuberculosis strain families such as
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis
complex, mycobacterial interspersed repetitive units-variable-number tandem
repeats (MIRU-VNTR), phylogeny evolution of M tuberculosis complex,
macroarray based on these sSNPs, differentiating between strain families,
bidirectional evolution, useful in epidemiology.
Guo, ShePing; Liu, SiGuo; Wang,
ChunLai; Shao,MeiLi; Gong, Qiang; Liu,JianDong. Prokaryotic
expression of fusion gene mpb64-Ag85B of Mycobacterium bovis in Escherichia
coli. Chinese Journal of Veterinary Science
and Technology. 2005; 35 (12): 946-949. ISSN: 1000-6419. Note:
In Chinese with an English summary.
Descriptors: cattle, Mycobacterium bovis, E. coli, genes,
gene expression, gene splicing, Ag85B, mpb64.
Guo, MingXing; Zhang, HanXie;
Chen, JianJun; Huang,Shen; Xu,GongHe; Chen, Ping Isolation and identification
of Mycobacterium bovis from swine sources. Chinese
Journal of Zoonoses. 2005; 21(10): 920-922. ISSN: 1002-2694.
Note: In Chinese with an English summary.
Descriptors: pigs, granulomatous lesions in various organs, disease
outbreaks, case reports, diagnosis, Mycobacterium bovis, drug resistance,
penicillins, rifampicin, streptomycin, susceptibility,
Hilty, Markus; Diguimbaye, Colette;
Schelling, Esther; Baggi, Franca; Tanner, Marcel; Zinsstag, Jakob. Evaluation
of the discriminatory power of variable number tandem repeat (VNTR) typing
of Mycobacterium bovis strains. Veterinary
Microbiology. 2005; 109 (3-4): 217-222. ISSN: 0378-1135
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/503320/description#description
NAL Call Number: SF601.V44
Abstract: The discriminatory power of variable number tandem repeat
(VNTR) typing based on 16 known loci (12 MIRUs, 3 ETRs and VNTR 3232) was
assessed for Mycobacterium bovis strains collected sequentially at
the slaughterhouse of N'Djamena, Chad. Of 67 M. bovis strains analyzed,
67% were clustered. In this study, VNTR typing was highly discriminative
with an overall allelic diversity (h(oa)) of 0.922. We defined five loci (ETR A, B, C and MIRU
26, 27) as highly (h > 0.25), two loci (MIRU 4, and VNTR 3232) as moderately
(0.11 < h < 0.25) and three loci (MIRU 16, 20, 3 1) as poorly (0.01
< h < 0.11) discriminative. Six loci (MIRU 2, 10, 23, 24, 39, and 40)
showed no polymorphism at all. VNTR typing of the five highly discriminative
loci (h = 0.917) proved to be most appropriate for first line typing of M.
bovis strains of
Descriptors: cattle, Mycobacterium bovis, collected 67 strains
at a slaughter house, allelic diversity, genetic polymorphism, N'Djamena,
Hope, J.C.;
URL: http://www.blackwellpublishing.com/journal.asp?ref=0019-2805&site=1
NAL Call Number: 448.3 IM6
Descriptors: Mycobacterium bovis, bacterial diseases, immunology,
CD8 positive T-cell, CD4-positive-T-cell, afferent lymph dendritic cell, CD26,
CD13, mannose receptor, expression, SIRP alpha, CD21, CD1b, expression, CD205,
187041-85-6, IL 10, secretion, interleukin 1 alpha, IL 12, secretion, phagocytosis.
Hughes, M.S.; Ball, N.W.; McCarroll,
J.; Erskine, M.; Taylor, M.J.; Pollock, J.M.; Skuce, R.A.; Neill, S.D. Molecular
analyses of mycobacteria other than the M. tuberculosis complex isolated
from
URL: http://www.sciencedirect.com/science/journal/03781135
Descriptors: Northern Ireland cattle, mycobacteria isolates from bovine
lymph nodes, PCR amplification of the 16S rRNA gene and reverse cross blot
hybridization, sequence analyses, MPB70, MPB 64, ESAT-6, CFP 10, Mycobacterium
nonchromogenicum, Mycobacterium malmoense, Mycobacterium bohemicum,
Mycobacterium paratuberculosis, Mycobacterium avium, Mycobacterium
kansasii, Mycobacterium holsaticum, Mycobacterium palustre,
Mycobacterium sp. IWGMT 90210, Mycobacterium sp. LIV-2129, a
potentially novel mycobacterial species (EMBL/GenBank/DDBJ Accession Number
AJ617495), UK.
Jiang Xiu yun;
Wang Chun feng; Wang Chun fang; He Zhao yang. Cloning and expression
of Mycobactetium bovis secreted protein MPB51 in Escherichia coli.
Weishengwu Xuebao. 2005; 45 (2): 298-300. ISSN: 0001-6209.
Note: In Chinese.
Descriptors: bovine tuberculosis, Mycobacterium bovis Vallee111
chromosomal DNA, PCR, genetic techniques, laboratory techniques, cloning,
Western blot, ELISA, SDS-PAGE, MPB51 amplifies, plasmid generation, pET28a
(+), pET28a-51transformed into competence E. coli BL21 (DE3), subunit
vaccine, DNA vaccine.
Jiang, XiuYun;
Wang, ChunFeng; Wang, ChunFang; He, ZhaoYang. Expression of Mycobacterium bovis Ag85A gene in Escherichia
coli. Chinese Journal of Veterinary Science
and Technology. 2005; 35(11): 875-878. ISSN: 1000-6419. Note:
In Chinese with an English summary.
Descriptors: Mycobacterium bovis, Escherichia coli,
DNA cloning, gene expression, genes, genetic transformation, plasmids, genetic
vectors, antigens, nucleotide-sequences, plasmids, pGEM T 85A and pET28a(+), digested using BamH I, EcoR I.
Khalid-Sendide;
Deghmane, A.E.; Pechkovsky, D; Av Gay, Y.; Talal, A.; Hmama, Z. Mycobacterium
bovis BCG attenuates surface expression of mature class II molecules through
IL-10-dependent inhibition of cathepsin S. Journal of Immunology. 2005; 175 (8): 5324-5332. ISSN:
0022-1767
URL: http://www.jimmunol.org/
NAL Call Number: 448.8 J8232
Abstract: We have previously shown that macrophage infection with
Mycobacterium tuberculosis and M. bovis bacillus Calmette-Guerin
(BCG) partially inhibits MHC class II surface expression in response to IFN-gamma.
The present study examined the nature of class II molecules that do in fact
reach the surface of infected cells. Immunostaining with specific abs that
discriminate between mature and immature class II populations showed a predominance
of invariant chain (Ii)-associated class II molecules at the surface of BCG-infected
cells suggesting that mycobacteria specifically block the surface export of
peptide-loaded class II molecules. This phenotype was due to inhibition of
IFN- gamma -induced cathepsin S (Cat S) expression in infected cells and the
subsequent intracellular accumulation of alpha beta class II dimers associated
with the Cat S substrate Ii p10 fragment. In contrast, infection with BCG
was shown to induce secretion of IL-10, and addition of blocking anti-IL-10
Abstracts to cell cultures restored both expression of active Cat S and export
of mature class II molecules to the surface of infected cells. Consistent
with these findings, expression of mature class II molecules was also restored
in cells infected with BCG and transfected with active recombinant Cat S.
Thus, M. bovis BCG exploits IL-10 induction to inhibit Cat S-dependent
processing of Ii in human macrophages. This effect results in inhibition
of peptide loading of class II molecules and in reduced presentation of mycobacterial
peptides to CD4+ T cells. This ability may represent an effective mycobacterial
strategy for eluding immune surveillance and persisting in the host.
Descriptors: humans, animal diseases, Mycobacterium bovis BCG,
Mycobacterium tuberculosis, immune response, immunopathology, gene
expression, interferon, interleukin 10, macrophages, cathepsins.
Koo, Hye Cheong; Park, Yong Ho;
Ahn, Jongsam; Waters, W. Ray; Palmer, Mitch V.; Hamilton, Mary Jo; Barrington,
George; Mosaad, Abdelaziz A.; Park, Kun Taek; Jung, Woo Kyung; Hwang, In Yeong;
Cho, Sang Nae; Shin, Sang Jae; Davis, William C. Use
of rMPB70 protein and ESAT-6 peptide as antigens for comparison of the enzyme-linked
immunosorbent, immunochromatographic, and latex bead agglutination assays
for serodiagnosis of bovine tuberculosis. Journal
of Clinical Microbiology. 2005; Sep 43 (9) 4498-4506. ISSN: 0095-1137
URL: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1489394
NAL Call Number: QR46.J6
Abstract: Current assays used to detect Mycobacterium bovis
infection lack accuracy, especially for recently infected animals, or are
impractical for rapid field diagnostic applications. To overcome these limitations
with serological assays, a synthetic peptide derived from early secretory
antigenic target 6 (ESAT6-p) and a recombinant major secreted immunogenic
protein (rMPB70) of M. bovis were used in an enzyme-linked immunosorbent
assay (EIA), an immunochromatographic assay (ICGA), and a latex bead agglutination
assay (LBAA). Sera from noninfected, M. bovis-infected,
or M. avium subsp. paratuberculosis-infected (by natural and
experimental routes) animals were evaluated. Receiver operating characteristic
analysis comparing optical density values from the EIA with results of bacterial
culture or skin test, the reference test, established suitable cutoff values
for assessing sensitivity and specificity. The EIA and LBAA, respectively,
had sensitivities of 98.6 and 94.8%, specificities of 98.5 and 92.6%, and
kappa values of 0.97 and 0.88 with ESAT6-p. The EIA, ICGA, and LBAA, respectively,
had sensitivities of 96.8, 83.0, and 86.7%, specificities of 90.1, 99.4, and
97.8%, and kappa values of 0.87, 0.85, and 0.83 with rMPB70. Examination
of serial samples of sera collected from experimentally M. bovis-infected
cattle and deer revealed that ESAT6-p-specific responses developed early after
infection whereas responses to rMPB70 developed later in the course of disease.
The advantage of the LBAA and ICGA as initial tests for multiple species
is a rapid reaction obtained in 2 to 3 h by LBAA or 20 min by ICGA without
species-specific secondary antibodies under field conditions, thus allowing
immediate segregation of suspect animals for further testing before culling.
Descriptors: Mycobacterium bovis, infection detection, synthetic
peptide derived from early secretory antigenic target 6 (ESAT6-p), recombinant
major secreted immunogenic protein (rMPB70), ELISA, immunochromatographic
assay (ICGA), latex bead agglutination assay (LBAA).
Kumar, Ashwani;
Chandolia, Amita; Chaudhry, Uma; Brahmachari, Vani; Bose, Mridula.
Comparison of mammalian cell entry operons of mycobacteria: in silico
analysis and expression profiling. FEMS Immunology
and Medical Microbiology. 2005; 43 (2): 185-195. ISSN: 0928-8244
URL: http://www.blackwellpublishing.com/journal.asp?ref=0928-8244&site=1
NAL Call Number: QR180.F46
Descriptors: mycobacteria host cell entry, mammalian cell entry operons,
pathogenic and saprophytic species, Mycobacterium smegmatis, Mycobacterium
bovis, Mycobacterium tuberculosis, silico analysis, Tt2B and Ttg2C domains,
differential expression under different culture conditions, mce1 operon, mce2
operon, mce3 operon, growth phase, redundancy in genome.
Lindstedt, Bjorn Arne. Multiple-locus
variable number tandem repeats analysis for genetic fingerprinting of pathogenic
bacteria. Electrophoresis. 2005; 26 (13): 2567-2582. ISSN:
0173-0835. Note: Literature review.
URL: http://www3.interscience.wiley.com/journal/110515951/abstract?CRETRY=1&SRETRY=0
NAL Call Number: QD79.E44E44
Descriptors: DNA fingerprinting, typing pathogenic bacteria, short
sequence repeats, SSR, variable number of tantem repeats, VNTR, genetic polymorphisms,
many bacterial families tested, Bordetella pertussis, Bacillus anthracis,
Salmonella typhimurium, Salmonella enterica, Yersinia pestis,
Escherichia coli strain-0157, Enterococcus faecium, Leptospira
interrogans, Staphylococcus aureus, Mycobacterium leprae,
Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium
avium paratuberculosis, Neisseria meningitides, Haemophilus
influenza, Pseudomonas aeruginosa, Borrelia burgdorferi.
Liu Zhi hui; Cai Xing shan; Zhu
Peng bo; Guan Ping; Xu Wan hua; Wu Long zhang. Study on species identification
of mycobacteria by gas chromatography analysis of whole-cell fatty acid.
Zhonghua Jiehe He Huxi Zazhi. 2005; 28
(6): 403-406. ISSN: 1001-0939. Note: In Chinese.
Descriptors: species identification, gas chromatography analysis,
accuracy and applicability, Mycobacterium bovis, Mycobacterium gastri,
Mycobacterium triviale, Mycobacterium smegmatis, Mycobacterium,
scrofulaceum, Mycobacterium gordnae.
Mackowiak, Philip
A.; Blos, Vera Tiesler; Aguilar, Manuel; Buikstra, Jane E. On
the origin of American tuberculosis. Clinical Infectious Diseases. 2005; 41 (4):
515-518,507. ISSN: 1058-4838
Descriptors: humans, animals, tuberculosis in the
Mishra, A.; Singhal, A.; Chauhan,
D.S.; Katoch, V.M.; Srivastava, K.; Thakral, S.S.; Bharadwaj, S.S.; Sreenivas,
V.; Prasad, H.K. Direct detection and identification of Mycobacterium
tuberculosis and Mycobacterium bovis in bovine samples by a novel
nested PCR assay: correlation with conventional techniques. Journal
of Clinical Microbiology. 2005 Nov.; 43 (11): 5670-5678. ISSN:
0095-1137
URL: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1489394
NAL Call Number: QR46 J6
Abstract: Mycobacterium tuberculosis and M. bovis infect
animals and humans. Their epidemiologies in developed and developing countries
differ, owing to differences in the implementation of preventive measures
(World Health Organization, 1999). Identification and differentiation of
these closely related mycobacterial species would help to determine the source,
reservoirs of infection, and disease burden due to diverse mycobacterial pathogens.
The utility of the hupB gene (Rv2986c in M. tuberculosis, or Mb3010c
in M. bovis) to differentiate M. tuberculosis and M. bovis
was evaluated by a PCR-restriction fragment length polymorphism (RFLP) assay
with 56 characterized bovine isolates (S. Prabhakar et al., J. Clin. Microbiol.
42:2724-2732, 2004). The degree of concordance between the PCR-RFLP assay
and the microbiological characterization was 99.0% (P < 0.001). A nested
PCR (N-PCR) assay was developed, replacing the PCR-RFLP assay for direct detection
of M. tuberculosis and M. bovis in bovine samples. The N-PCR
products of M. tuberculosis and M. bovis corresponded to 116
and 89 bp, respectively. The detection limit of mycobacterial DNA by N-PCR
was 50 fg, equivalent to five tubercle bacilli. M.
tuberculosis and/or M. bovis was detected in 55.5% (105/189) of
the samples by N-PCR, compared to 9.4% (18/189) by culture. The sensitivities
of N-PCR and culture were 97.3 and 29.7, respectively, and their specificities
were 22.2 and 77.7%, respectively. The percentages of animals or samples
identified as infected with M. tuberculosis or M. bovis by N-PCR
and culture reflected the clinical categorizations of the cattle (P of <0.05
to <0.01). Mixed infection by N-PCR was detected in 22 animals, whereas
by culture mixed infection was detected in 1 animal.
Descriptors: Mycobacterium tuberculosis, Mycobacterium bovis,
animals, humans, identification, differentiation, epidemiology, source, disease
reservoirs, disease burden due to multiple infections, RFLP assay, 56 bovine
isolates, nested PCR assay, 116 and 89 bp, sensitivities.
Monincova, M.; Jesenska, A.; Pavlova,
M .; Strouhal, M.; Tisinska, I.; Chaloupkova, R.; Prokop, Z.; Bartos, M.;
Pavlik, I.; Rychlik, I.; Mobius, P.; Nagata, Y.; Damborsky, J. Mycobacterial haloalkane dehalogenases. FEBS
Journal. 2005; 272 (Suppl. 1): 514-515. ISSN: 1742-464X1742-4658.
Note: A poster at the 30th Congress of the Federation of European Biochemical
Societies (FEBS)/9th IUBMB Conference,
Descriptors: Mycobacterium species, Escherichia coli,
species, expression system, Mycobacterium tuberculosis, Mycobacterium smegmatis
strain-MC2-155; Mycobacterium avium paratuberculosis strain-K10; Mycobacterium
bovis strain-5032-66 dmbA-gene, haloalkane dehalogenase 95990-29-7, EC-3.8.1.5.
Mostowy, Serge; Inwald, Jackie;
Gordon, Steve; Martin, Carlos; Warren, Rob; Kremer, Kristin; Cousins, Debby;
Behr, Marcel A. Revisiting the evolution of Mycobacterium
bovis. Journal of Bacteriology.
2005; 187 (18): 6386-6395. ISSN: 0021-9193
Descriptors: Mycobacterium bovis, genomics, variability, strain
differences, distinct distributions of disease, polymorphisms, broad host
capacity, variety of mammals.
Murry, Jeffrey;
Sassetti, Christopher M.; Moreira, Jonathan; Lane, James; Rubin, Eric J.
A new site-specific integration system for mycobacteria.
Tuberculosis (
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/638428/description?navopenmenu=-2
Descriptors: Streptomyces phi C31 integration system; integrate
vector DNA into Mycobacterium smegmatis, Mycobacterium bovis
BCG, and Mycobacterium tuberculosis, site-specific recombination, stable
transformants, useful in studying mycobacterial genetics.
Nascimento,
Ivan P.; Leite, Luciana C.C. The effect of passaging
in liquid media and storage on Mycobacterium bovis - BCG growth capacity
and infectivity. FEMS Microbiology
Letters. 2005; 243(1): 81-86. ISSN: 0378-1097
URL:
http://www.elsevier.com/wps/find/journaldescription.cws_home/506058/description#description
NAL Call Number: QR1.F44
Descriptors: Mycobacterium bovis bacille Calmette-Guerin (BCG)
Moreau strain; recombinant BCG (rBCG) vaccine preparation strain; effects
of freezing, storage and thawing; three rounds of freezing and thawing limit
ability for growth; culture density; macrophage infectivity tested, important
factors, use fresh, low-passage and/or growth and infection capacity-controlled
vaccine stocks.
URL: http://www.blackwell-synergy.com/loi/jam
NAL Call Number: QR1.J687
Descriptors: bovine tuberculosis, zoonotic aspects, new information
about Mycobacterium bovis, recent developments, pathogenesis, epidemiology,
disease eradication, diagnosis, vaccination.
Newell, D.;
Belcher, T. Med Vet Net: integrating research on zoonoses.
GVJ-Government Veterinary Journal. 2005; 15
(2): 12-17. ISSN: 0269-5545
URL: http://www.defra.gov.uk/gvs/publications/gvj/pdf/gvj-vol1701.pdf (PDF | 731KB)
Descriptors: animal diseases, pathogens, zoonotic organisms,
Echinococcus, Escherichia coli; Mycobacterium bovis,
listerellosis, Salmonella infections, studies, trichinellosis, viral
infections; zoonotic infections,
Olin, Michael
R.; Choi, K. Hwa; Lee, Jinhee; Molitor, Thomas W. Gamma delta T-lymphocyte cytotoxic activity against Mycobacterium
bovis analyzed by flow cytometry. Journal
of Immunological Methods. 2005; 297 (1-2): 1-11. ISSN: 0022-1759
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/506022/description#description
Descriptors: post animal vaccination, gamma delta T lymphocytes, response
to Mycobacterium bovis BCG, proliferation of IFN gamma production,
innate cytolytic functions, cytolytic assay, flow cytometry, K562 cells as
targets, optimizing the assay, conclusion was that the assay is sensitive
and reliable for cytolytic activity of gamma delta T lymphocytes.
Prasad, H.K.; Singhal, A.; Mishra,
A.; Shah, N.P.; Katoch, V.M.; Thakral, S.S.; Singh, D.V.; Chumber, S.; Bal,
S.; Aggarwal, S.; Padma, M.V.; Kumar, S.; Singh, M.K.; Acharya, S.K. Bovine
tuberculosis in
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/638428/description?navopenmenu=-2
Descriptors: tuberculosis, Mycobacterium tuberculosis infection,
diagnosis, transmission, genetics, diagnosis, etiology, Mycobacterium bovis,
nested PCR, genetic techniques, diagnostic assay techniques, clinical techniques,
mixed infections, zoonotic diseases.
Perez, O.A. Mycobacterium
avium implicated in zoonoses. Revista de Medicina Veterinaria
Buenos Aires. 2005; 86 (6): 263-264. ISSN: 0325-6391. Note: In Spanish.
Descriptors: avian tuberculosis, Mycobacterium avium, Mycobacterium
avium complex, taxonomy, subspecies, indentification, seroagglutination
testing, immunological methods, infection prevalence, Crohn’s disease, paratuberculosis,
review article,.
Price, S.J.;
Hope, J.C.; Howard, C.J. Bovine WC1+gamma delta T cells are synergistically
stimulated by IL-12 and IL-18 to secrete high levels of IFN gamma. Immunology.
2005; 116 (Suppl. 1): 77. ISSN: 0019-2805. Note: Abstract, Annual Congress
of the British-Society-for-Immunology,
URL: http://www.blackwellpublishing.com/journal.asp?ref=0019-2805&site=1
NAL Call Number: 448.3 IM6
Descriptors: cytokines, interferon-gamma, CD40, interleukin 18; interleukin-12,
mycobacterial infections, synergistic stimulation, Mycobacterium bovis.
Rosenkrands, Ida; Agger, Else
Marie; Olsen, Anja W.; Korsholm, Karen S.; Andersen, Claire Swetman; Jensen,
Klaus T.; Andersen, Peter. Cationic liposomes containing mycobacterial
lipids: a new powerful Th1 adjuvant system. Infection
and Immunity. 2005; 73 (9): 5817-5826. ISSN: 0019-9567
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: The immunostimulation provided by the mycobacterial cell
wall has been exploited for many decades, e.g., in Freund's complete adjuvant.
Recently, the underlying mechanism behind this adjuvant activity, including
Toll receptor signaling, has begun to be unraveled, confirming the potential
of mycobacterial constituents to act as adjuvants. In this study, the immunostimulatory
properties of a Mycobacterium bovis BCG lipid extract were tested for
their adjuvant activity. Administration of the lipids in dimethyl dioctadecyl
ammonium bromide-based cationic liposomes induced a powerful Th1 response
characterized by markedly elevated antigen-specific immunoglobulin G2a (IgG2a)
isotype antibodies and substantial production of gamma interferon. The adjuvant
formulation (designated mycosomes) elicited high levels of gamma interferon
both in C57BL/6 as well as in Th2-prone BALB/c mice. Furthermore, the mycosomes
induced immune responses to protein antigens from several sources including
Mycobacterium tuberculosis, Chlamydia muridarum, and tetanus
toxoid. In a tuberculosis challenge model, the mycosomes combined with the
Ag85B-ESAT-6 fusion protein were demonstrated to have a unique ability to
maintain sustained immunological memory at a level superior to live BCG.
Descriptors: adjuvant activity, mycobacterial cell wall, Freunds complete
adjuvant, immunostimulatory properties, Mycobacterium bovis BCG lipid
extract, Th2-prone Balb/c mice.
Skuce, R.A.; McDowell, S.W.; Mallon,
T.R.; Luke, B.; Breadon, E.L.; Lagan, P.L.; McCormick, C.M.; McBride, S.H.;
Pollock, J.M. Discrimination of isolates of Mycobacterium bovis
in
URL: http://veterinaryrecord.bvapublications.com/
NAL Call Number: 41.8 V641
Descriptors: Mycobacterium bovis, disease transmission,
genetic techniques and protocols,
Wheeler, Paul R.; Coldham, Nicholas
G.; Keating, Lisa; Gordon, Stephen V.; Wooff, Esen E.; Parish, Tanya; Hewinson,
R. Glyn. Functional demonstration of reverse transsulfuration in the Mycobacterium
tuberculosis complex reveals that methionine is the preferred sulfur source
for pathogenic mycobacteria. Journal of Biological
Chemistry. 2005; 280 (9): 8069-8078. ISSN: 0021-9258.
URL: http://www.jbc.org/
Abstract: Methionine can be used as the sole sulfur source by the Mycobacterium
tuberculosis complex although it is not obvious from examination of the
genome annotation how these bacteria utilize methionine. Given that genome
annotation is a largely predictive process, key challenges are to validate
these predictions and to fill in gaps for known functions for which genes
have not been annotated. We have addressed these issues by functional analysis
of methionine metabolism. Transport, followed by metabolism of S-35 methionine
into the cysteine adduct mycothiol, demonstrated the conversion of exogenous
methionine to cysteine. Mutational analysis and cloning of the Rv1079 gene
showed it to encode the key enzyme required for this conversion, cystathionine
gamma-lyase (CGL). Rv1079, annotated metB, was predicted to encode cystathionine
gamma-synthase (CGS), but demonstration of a gamma-elimination reaction with
cystathionine as well as the gamma-replacement reaction yielding cystathionine
showed it encodes a bifunctional CGL/CGS enzyme. Consistent with this, a
Rv1079 mutant could not incorporate sulfur from methionine into cysteine,
while a cysA mutant lacking sulfate transport and a methionine auxotroph was
hypersensitive to the CGL inhibitor propargylglycine. Thus, reverse transsulfuration
alone, without any sulfur recycling reactions, allows M. tuberculosis
to use methionine as the sole sulfur source. Intracellular cysteine was undetectable
so only the CGL reaction occurs in intact mycobacteria. Cysteine desulfhydrase,
an activity we showed to be separable from CGL/CGS, may have a role in removing
excess cysteine and could explain the Ability of M. tuberculosis to
recycle sulfur from cysteine, but not methionine.
Descriptors: Escherichia coli strain DH5 alpha, Mycobacterium
tuberculosis strain H37Rv; Mycobacterium bovis strain-BCG-Pasteur,
pathogenic strain metablolism, biochemistry of methionine as sulfur source.
Winters, A. Driver, C.; Macaraig,
M.; Clark, C.; Munsiff, S.S.; Pichardo, C.; Driscoll, J.; Salfinger, M.; Kreiswirth,
B.; Jereb, J.; LoBue, P.; Lynch, M. Human tuberculosis caused by Mycobacterium
bovis New York City, 2001-2004. Morbidity and
Mortality Weekly Report. 2005; 54 (24): 605-608. ISSN: 1057-5987
Descriptors: Mycobacterium bovis, zoonotic disease potential,
epidemiology, infant contracted the disease, diagnostic techniques, genetic
techniques, spoligotyping.
Yeruva, Veena
C.; Sundaram, C.A.S. Sivagami; Sritharan, Manjula. Effect of iron concentration on the expression and activity of
catalase-peroxidases in mycobacteria. Indian
Journal of Biochemistry and Biophysics. 2005; 42 (1): 28-33.
ISSN: 0301-1208
Descriptors: iron sufficient and deficient concentration in growth
media, expression and activity of the different isoforms, Mycobacterium
bovis BCG, M. smegmati, M. fortuitum, M. kansasii,
M. vaccae, differences in catalase/peroxidase activity, susceptibility
to heat inactivation, isoforms had variable heat responses.
Young, Jamie-S.;
Gormley, Eamonn;
URL: http://www.pubmedcentral.nih.gov/tocrender.fcgi?action=archive&journal=83
NAL Call Number: 448.3 Ap5
Abstract: PCR primers specific for the Mycobacterium tuberculosis
complex were used to detect the presence of Mycobacterium bovis BCG
(Pasteur) in soil microcosms and Mycobacterium bovis in environmental
samples taken from a farm in
Descriptors: Mycobacterium bovis, environmental sampling of
soils, PCR primers, areas of badger setts had highest levels of gene persistance,
10 day persistence, optimal conditions, Ireland.
Young, Jamie
S.; Gormley, Eamonn;
URL: http://www.pubmedcentral.nih.gov/tocrender.fcgi?action=archive&journal=83
NAL Call Number: 448.3 Ap5
Descriptors: PCR primers specific to Mycobacterium tuberculosis
complex, Mycobacterium bovis, environmental samples, soil microcosms,
farm land, around badger setts locations, 16SrRNA sequences, evidence of viable
cells in soil, dead cells persisted for less than 10 days, optimal moist soil
survival temperature was 37degrees C,
Zanini, M.S.; Moreira, E.C.; Salas,
C.E.; Lopes, M.T.P.; Barouni, A.S.; Roxo, E.; Telles, M.A.; Zumarraga, M.J.
Molecular typing of Mycobacterium bovis isolates from south-east
NAL Call Number: 41.8 Z52
Descriptors: dairy cattle, beef cattle, bovine tuberculosis, Mycobacterium
bovis, pathogen identification, microbial genetics, strains, genetic polymorphism,
molecular genetics, antibiotic resistance, diagnostic techniques, spoligotyping,
ethionamide rifampicin, isoniazid, strain differences, disease surveillance,
diagnostic-techniques, post slaughter tissue collection, identification of
163 strains, polymerase chain reaction (PCR) and microbiological tests,
252 tuberculous-like lesions, 3 genotyping techniques, IS6110-restriction
fragment length polymorphism (RFLP), polymorphic guanine-cytosine-rich sequence
(PGRS)-RFLP and direct repeat (DR)-spoligotyping, fails to show a correlation
between main cluster found by the 3 techniques, Brazil.
Zanini, M.S.; Moreira, E.C.; Salas,
C.E.; Lopes, M.T.P.; Barouni, A.S.; Roxo, E.; Telles, M.A.; Zumarraga, M.J.
Molecular typing of Mycobacterium bovis isolates from south-east
NAL Call Number: 41.8 Z52
Descriptors: dairy cattle, beef cattle, bovine tuberculosis, Mycobacterium
bovis, pathogen identification, microbial genetics, strains, genetic polymorphism,
molecular genetics, antibiotic resistance, diagnostic techniques, spoligotyping,
ethionamide rifampicin, isoniazid, strain differences, disease surveillance,
diagnostic-techniques, post slaughter tissue collection, identification of
163 strains, polymerase chain reaction (PCR) and microbiological tests,
252 tuberculous-like lesions, 3 genotyping techniques, IS6110-restriction
fragment length polymorphism (RFLP), polymorphic guanine-cytosine-rich sequence
(PGRS)-RFLP and direct repeat (DR)-spoligotyping, fails to show a correlation
between main cluster found by the 3 techniques, Brazil.
Zumarraga, M.J.;
Meikle, V.; Bernardelli, A.; Abdala, A.; Tarabla, H.; Romano, M.I.; Cataldi,
A. Use of touch-down polymerase chain reaction
to enhance the sensitivity of Mycobacterium bovis detection.
Journal of Veterinary Diagnostic Investigation.
2005; 17 (3): 232-238. ISSN: 1040-6387
URL: http://jvdi.org/
NAL Call Number: SF774.J68
Descriptors: Mycobacterium bovis, PCR, detection, diagnosis,
sensitivity of testing.
2004
Adcock, V.;
Durr, P.A. Use of scalable vector graphics for
a web-delivered interactive digital atlas of bovine tuberculosis.
GISVET' 04: Second International Conference on the Applications of
GIS and Spatial Analysis to Veterinary Science,
Abstract: Scalable vector graphics (SVG) is a new XML-based web technology
combining high quality graphics, enhanced browser-based interactivity and
rapid load times. This technology is useful for the production of interactive
disease maps. The author describes its use for the successful implementation
of an historical atlas of bovine tuberculosis in
Descriptors: Mycobacterium tuberculosis, computer programs,
Barouni, A.S.;
Augusto, C.J.; Lopes, M.T.P.; Zanini, M.S.; Salas, C.E. A
pncA polymorphism to differentiate between Mycobacterium bovis and
Mycobacterium tuberculosis. Molecular and Cellular Probes. 2004; 18 (3): 167-170.
ISSN: 0890-8508
Descriptors: pyrazinamidase gene coding, polymorphic site preserved
in Mycobacterium bovis, synthesized primers, 180 pb fragment, 726 bp
fragment with pncA gene, PCR, digestion with Eco065I, differential identification
of unique fragments for each species.
Brandt, L.; Skeiky, Y.A.W.; Alderson,
M.R.; Lobet, Y.; Dalemans, W.; Turner, O.C.; Basaraba, R.J.; Izzo, A.A.; Lasco,
T.M.; Chapman, P.L.; Reed, S.G.; Orme, I.M. The protective effect of the
Mycobacterium bovis BCG vaccine is increased by coadministration with
the Mycobacterium tuberculosis 72-kilodalton fusion polyprotein Mtb72F
in M. tuberculosis-infected guineapigs. Infection
and Immunity. 2004; 72 (11): 6622-6632. ISSN: 0019-9567
URL: http://iai.asm.org/cgi/content/Abstractstract/72/11/6622
NAL Call Number: QR1.I57
Abstract: A tuberculosis vaccine candidate consisting of a 72-kDa polyprotein
or fusion protein based upon the Mtb32 and Mtb39 antigens of Mycobacterium
tuberculosis and designated Mtb72F was tested for its protective capacity
as a potential adjunct to the Mycobacterium bovis BCG vaccine in the
mouse and guineapig models of this disease. Formulation of recombinant Mtb72F
(rMtb72F) in an AS02A adjuvant enhanced the Th1 response to BCG in mice but
did not further reduce the bacterial load in the lungs after aerosol challenge
infection. In the more stringent guineapig disease model, rMtb72F delivered
by coadministration with BCG vaccination significantly improved the survival
of these animals compared to BCG alone, with some animals still alive and
healthy in their appearance at >100 weeks post-aerosol challenge. A similar
trend was observed with guinea pigs in which BCG vaccination was boosted by
DNA vaccination, although this increase was not statistically significant
due to excellent protection conferred by BCG alone. Histological examination
of the lungs of test animals indicated that while BCG controls eventually
died from overwhelming lung consolidation, the majority of guinea pigs receiving
BCG mixed with rMtb72F or boosted twice with Mtb72F DNA had mostly clear lungs
with minimal granulomatous lesions. Lesions were still prominent in guinea
pigs receiving BCG and the Mtb72F DNA boost, but there was considerable evidence
of lesion healing and airway remodeling and reestablishment. These data support
the hypothesis that the coadministration or boosting of BCG vaccination with
Mtb72F may limit the lung consolidation seen with BCG alone and may promote
lesion resolution and healing. Collectively, these data suggest that enhancing
BCG is a valid vaccination strategy for tuberculosis that is worthy of clinical
evaluation.
Descriptors: guinea pigs, Mycobacterium bovis, Mycobacterium
tuberculosis, fusion proteins, antigens, bacterial proteins, candidate
vaccines, DNA vaccines, immune response, immunity, immunization, lungs, niceritrol,
tuberculosis, vaccination, vaccine development.
Broxmeyer, L. Is mad cow disease
caused by a bacteria? Medical
Hypotheses. 2004; 63(4): 731-739. ISSN: 0306-9877
Descriptors: TSE, BSE, CJD, scrapie, transmissible spongiform encephalopathies,
prion, causes of disease, misfolded proteins, bacterial DNA, Mycobacterium
bovis, isolation from clinical and histopathological signs of mad cow,
UK areas of BSE where M. Bovis is highest, tuberculosis spongiform
encephalitis, Mycobacterium avium, ssp paratuberculosis (fowl
tuberculosis), mycobacteria hypothesis for mad cow disease.
Chambers, M.A.;
Gavier-Widen, D.; Hewinson, R.G. Antibody bound to the surface
antigen MPB83 of Mycobacterium bovis enhances survival against high
dose and low dose challenge. FEMS Immunology and
Medical Microbiology. 2004; 41 (2): 93-100. ISSN: 0928-8244
URL: http://www.blackwellpublishing.com/journal.asp?ref=0928-8244&site=1
NAL Call Number: QR180.F46
Abstract: Tuberculosis caused by infection with Mycobacterium
tuberculosis or Mycobacterium bovis is a significant disease of
man and animals. Whilst cellular immunity is the major immunological component
required for protection against these organisms, recent reports have suggested
that monoclonal antibodies can modify infection with M. tuberculosis.
To test whether the same was true for M. bovis infection, we determined
the effect of preincubation of M. bovis with a monoclonal antibody
on subsequent intravenous infection of mice. Antibodies bound to the surface
of M. bovis increased the survival time of mice infected with M.
bovis and changed the morphology of granulomas and the distribution of
acid-fast bacilli in the lung. These studies suggest that antibodies directed
to the surface of virulent mycobacteria can modulate their virulence in vivo.
Descriptors: Mycobacterium bovis, animal pathogenic bacteria,
infection, virulence, monoclonal antibodies, surface antigens, mice.
Coffey, Michael
Joseph; Phare, Susan M.; Peters-Golden, Marc. Role of leukotrienes in killing of Mycobacterium bovis by
neutrophils. Prostaglandins Leukotrienes and Essential Fatty Acids.
2004; 71 (3): 185-190. ISSN: 0952-3278
Descriptors: Mycobacterium bovis, host defense, phagocytosis
an killing processes, leukotrines (LT), role in killing, LT synthesis inhibitor
MK 886 affected ability of neutrophils to kill Mycobacterium bovis,
LT increased when neutrophils were incubated with Mycobacterium bovis.
Collins, D.M.C.;
For, R.; Skou, B.; Collins, L.; Bassett, S.; de Lisle, G.W. Signature tag mutagenesis with Mycobacterium bovis.
Abstracts of the General Meeting of the American Society
for Microbiology. 2004: 104: 628. ISSN: 1060-2011. Note: 104th
General Meeting of the American Society for Microbiology,
NAL Call Number: QR1.A5
Descriptors: guinea pigs, Mycobacterium bovis, mutagenesis.
Denis, Michel;
Wedlock, D. Neil; Buddle, Bryce M. Ability of
T cell subsets and their soluble mediators to modulate the replication of
Mycobacterium bovis in bovine macrophages. Cellular
Immunology. 2004; 232 (1-2): 1-8. ISSN: 0008-8749
Descriptors: vaccinated cattle, peripheral blood mononuclear cells
(PBMCs), Mycobacterium bovis BCG, Mycobacterium bovis virulent
strain, modulation of replication between exposure
of cells from vaccinated to virulent pathogen, compared to contols, neutralizing
antibody IFN-gamma, addition of T-cells, neutralizing of nitric oxide by inclusion
of monomethyl-L arginine, immune resistance.
Dhama, K.; Bansal,
M.P.; Rathore, R.; Ram, G.C. Evaluation of the role of Con-A and
PHA-P induced leucocyte conditioned medium in activating bovine blood monocytes
pulsed with Mycobacterium bovis BCG. Journal
of Immunology and Immunopathology. 2004; 6 (2): 24-27. ISSN:
0972-0561.
Descriptors: calves, tuberculosis free, intramuscular inoculation,
pure culture of Mycobacterium bovis, sensitization by DTH skin testing,
ELISA, LTT using PPD as antigen, blood monocytes, in vitro stimulated, role
of concanavalin A (Con-A) and phytohaemagglutinin (PHA-P) induced leukocyte
conditioned medium, cell behaviors, phagocytosis, immune phagocytosis, antibody
dependent cellular cytotoxicity, nitrite production, intracellular killing
of M. bovis BCG.
Diguimbaye, C.; Schelling, E.;
Pfyffer, G.E.; Baggi, F.; Ngandolo, R.; Ndoutamia, G.; Tanner, M.; Zinsstag,
J. Premiers isolements de mycobacteries tuberculeuses chez l'homme et
l'animal au Tchad. [First isolation of tuberculous mycobacteria
in man and animals in
Descriptors: first isolation and identification of Mycobacterium
bovis, Mycobacterium tuberculosis, antibiotic resistance, pyrazinamide,
control policies needed,
Fritsche, A.;
Engel, R.; Buhl, D.; Zellweger, J.P. Mycobacterium bovis
tuberculosis: from animal to man and back. International
Journal of Tuberculosis and Lung Disease. 2004; 8 (7): 903-904.
ISSN: 1027-3719. Note: In English with French and Spanish summaries.
Descriptors: humans, cattle, other infected animals, Mycobacterium
bovis, strains, zoonotic disease, disease transmission from animal
to human and back to animal, case reports, clinical aspects, disease course,
disease transmission, exposure, human diseases, strains, tuberculosis, Switzerland.
Haddad, Nadia;
Masselot, Monique; Durand, B. Molecular differentiation of Mycobacterium
bovis isolates. Review of main techniques and applications. Research in Veterinary Science. 2004; 76 (1): 1-18.
ISSN: 0034-5288
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/623070/description#description
NAL Call Number: 41.8 R312
Descriptors: Mycobacterium bovis, typing techniques, genetic
species Mycobacterium tuberculosis complex, genome studies, IS6110
insertion sequence markers, direct repeat region, poly (GC)rich sequences,
variable number tandem repeats sequences, description of techniques, examples
of typing application reviewed, epidemiology problems.
Hammer, P.
Heat inactivation of classical mycobacteria in milk
- a historical review. Bulletin of
the International Dairy Federation. 2004; (392): 42-48. ISSN:
0250-5118. Note: International workshop on "Revisiting Heat Resistance
of Microorganisms in Milk,
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis,
bacterial inactivation in milk, high temperature short time pasteurization,
research variables in articles, food contamination, food safety, bacterial
heat tolerance, historical literature review.
Hope, J.C.; Thom, M.L.; McCormick,
P.A.; Howard, C.J. Interaction of antigen presenting
cells with mycobacteria. Veterinary Immunology and Immunopathology. 2004; 100
(3/4): 187-195. ISSN: 0165-2427. Note: Host Pathogen Interactions. Plenary
Papers Presented at the CVIG Session of the AVTRW Annual Conference,
URL: http://www.elsevier.com/wps/find/journalabstracting.cws_home/503319/abstracting
NAL Call Number: SF757.2.V38
Descriptors: cattle, Mycobacterium bovis, Mycobacterium
bovis BCG strain, bovine antigen presenting cells, dendritic cell infection
with mycobacteria, cell-based immunity, antigens, innate and adaptive immune
responses induced, cytokines, immune response, interleukins, macrophage activation,
major histocompatibility complex, strain, T-lymphocytes, tumour necrosis factor.
Jacobs, William R .Jr; Bloom,
Barry; Kalpana, Ganjam V.; Cirillo, Jeffrey D.; McAdam, Ruth. Insertional mutations in mycobacteria. Official
Gazette of the
URL: http://www.uspto.gov/web/menu/patdata.html
Abstract: A mutated mycobacterium selected from the class consisting
of mutated M. bovis BCG, mutated M. tuberculosis, and mutated
M. leprae. The mutation of M. bovis BCG, M. tuberculosis,
or M. leprae is preferably effected through an insertional mutation
of a mycobacterial gene. The insertional mutagenesis may be effected, for example, through illegitimate recombination or
by a mycobacterial transposon. Such mutated mycobacteria may then be transformed
with an expression vector(s) containing a complement gene to the gene which
is mutated, and preferably also including a heterologous gene.
Descriptors: mycobacteria, Mycobacterium bovis BCG, Mycobacterium
tuberculosis, Mycobacterium leprae, insertional mutations.
Jiang, XiuYun;
Wang, ChunFeng; Wang, ChunFang; He, ZhaoYang. Cloning and sequence analysis of Mycobacterium bovis Ag85A
gene. Chinese Journal of Veterinary Science and Technology.
2004; 34 (9): 21-24. ISSN: 1000-6419. Note: In Chinese with an English
summary.
Descriptors: Mycobacterium bovis strain Vallee111, DNA extraction,
secreted Ag85A gene, amplified using PCR, clone vectorpGEM-T,85A, cloned into
pGEM-T vector using T-A clone technique, immunogenicity, gene expression,
DNA sequencing.
Koo, Hye Cheong; Park, Yong Ho;
Ahn, Jongsam; Waters, W. Ray; Hamilton, Mary Jo; Barrington, George; Mosaad,
Abdelaziz A.; Palmer, Mitch V.; Shin, Sang; Davis, William C. New latex
bead agglutination assay for differential diagnosis of cattle infected with
Mycobacterium bovis and Myobacterium avium subsp. paratuberculosis.
Clinical and Diagnostic Laboratory Immunology.
2004; 11 (6): 1070-1074. ISSN: 1071-412X
Descriptors: cattle, identification of animals infected with Mycobacterium
bovis, Mycobacterium avium subsp. paratuberculosis, current
assays not sensitive and specific to identify diseased animals, latex bead
agglutination assay (LBAA) using specific immunodominant epitope (ESAT6-p)
of M. bovis, compared assay to culture method and skin test, experimental
infection and non-infected animals, species specific diagnosis, sera testing,
data suggest a rapid, sensitive and specific assay can be developed.
Kurabachew, Mekonnen; Enger, Oivind;
Sandaa, Ruth Anne; Skuce, Robin; Bjorvatn,-Bjarne. A multiplex
polymerase chain reaction assay for genus-, group- and species- specific detection
of mycobacteria. Diagnostic Microbiology and Infectious
Disease. 2004; 49(2): 99-104. ISSN: 0732-8893
Descriptors: multiplex PCR assay, single step, differential between
species, Mycobacterium tuberculosis complex and other non TB
Mycobacterium species using 16S and 23S rDNA, and, Mycobacterium
tuberculosis and Mycobacterium bovis species using oxyl? gene, 156 clinical samples and reference stains used, Mycobacterium
africanum, oxyRTB2.1 loxyRMB-1 primers, 1pg of purified genomic DNA for
identification.
Kutalik, Zoltan;
Razaz, Moe; Inwald, Jackie; Gordon, Steve V.; Wolkenhauer, Olaf. A
novel statistical approach in comparative genomics to reveal new immunogenic
antigens in M. bovis. Tissue Antigens.
2004; 64 (4): 427. ISSN: 0001-2815. Note: Meeting abstract. 1st International
Conference on Basic and Clinical Immunogenomics,
Descriptors: Mycobacterium bovis, comparative genomics, cattle
pathogen, molecular genetics methods and techniques, immunology, immunogenic
antigens, immunostimulant drug, tuberculin PPD, diagnostic antigen, Bayesian
analysis, PCR, spoligotype-specific-deletion.
Kutalik, Zoltan; Inwald, Jacqueline;
Gordon, Steve V.; Hewinson, R. Glyn; Butcher, Philip; Hinds, Jason; Cho, Kwang
Hyun; Wolkenhauer, Olaf. Advanced significance analysis
of microarray data based on weighted resampling: a comparative study and application
to gene deletions in Mycobacterium bovis. Bioinformatics
(
Descriptors: methods, analyzing microarray data, differences in gene
expression levels, normalizezed channel intensity levels, different experimental
conditions, SAM, regularized t-test, mixture modeling, Wilk’s lambda score,
variance stabilization, weighted resampling approach, gene deletions, Mycobacterium
bovis, assumptions, model structure, computation, applicability.
URL: http://www.nal.usda.gov/awic/pubs/TB/TBMain.htm
NAL Call Number: aHV4701.A94 no. 2004-01
Abstract: The focus of this publication is on information related to
tubercular diseases of animals caused by the bacterial genus Mycobacterium.
Livestock diseases are mostly caused by Mycobacterium bovis and the
Mycobacterium tuberculosis complex. Many species of animals are included:
large ruminants, wildlife, wild animals as disease reservoirs, deer, elephants,
birds, fish, etc. Topics are varied and include clinical aspects of the disease,
the disease process, disease prevention and control, vaccines, immunology,
bacterial genetics, zoonotic aspects, etc.
Descriptors: tuberculosis in animals, bibliography, Mycobacterium sp,
Mycobacterium avium, Mycobacterium bovis, zoonoses, production
animals, zoo animals, wild animals, disease control, Mycobacterium tuberculosis
complex, microbial genetics, disease incidence worldwide, control programs
worldwide, immune response, wild animal vectors, treatments, animal disease
models, aquatic animals, diagnostic methods, disease pathology, disease incidence
worldwide.
Lucca, E.; Canal, A.M.; Pachoud,
J.C.; Gollan, A.; Bergamasco, M.; Latini, M.; Lopez, M.; Nicola, A.; Tomatis,
I.; Scarpin, V. Diagnostico de tuberculosis bovina: correlacion entre
pruebas diagnosticas. [Diagnosis of bovine tuberculosis: correlation
between different diagnostic tests.] Veterinaria
Descriptors: dairy cattle; Mycobacterium bovis; blood sampling,
diagnostic tests; correlation between tuberculin skin test, bacteriological
cultures, microscopic lesions of lymph nodes and other organs, and interferon-gamma
assay; interferon-gamma assay not sufficient at detecting M. bovis,
Argentina.
Manuel-Tibata, Victor; Eugenia-Gonzalez,
Clara; German-Rodriguez, Juan; del Portillo, Patricia.
[Transcriptional analysis of genetic region RvD1 of Mycobacterium
bovis.] Revista Colombiana de Biotecnologia. 2004;
6(2): 62-66. ISSN: 0123-3475. Note: In Spanish.
Descriptors: Mycobacterium bovis, 99.9% of genomic identity
with M. tuberculosis, M. africanum, M. microti, 2 genetic
regions deleted in M. tuberculosis--H37Rv: RvD1, RvD2, RNA from M.
bovis BCG, Rtq-PCR, ORF1, ORF2 and Rv2024, were transcribed constitutively,
RvD1 possible role in pathogenesis, interaction with both cattle and humans.
Milian-Suazo,
F.; Serna-Gonzalez, C.O.; Banda-Ruiz, V.; Robles P., G.; Arriaga-Diaz, C.;
Anaya-Escalera, A.M. Genetic stability of a Mycobacterium
bovis strain by serial infections in guinea pigs. Tecnica
Pecuaria en
Descriptors: guinea pigs, Mycobacterium bovis, cattle isolated
strain, disease model, “direct repeat” region variability, serial passage,
intraperitoneal inoculation, 103 pathogens/animal, non-lethal
mutation developed during bacterial reproduction.
Pate, Mateja;
Zdovc, Irena; Pirs, Tina; Krt, B.; Ocepek, M. Isolation and characterisation of Mycobacterium avium and
Rhodococcus equi from granulomatou lesions of swine lymph nodes in
Descriptors: cattle; swine; lymph nodes; mixed infections; Mycobacterium
hominissuis (IS901-, IS1245+ genotype); Mycobacterium avium avium (IS901+,
IS1245+ genotype); typed using IS1245, IS901 and FR300 PCR; Rhodococcus
equi isolates; tested for virulence-associated antigens (VapA and VapB).
Roring, Solvig; Scott, Alistair
N.; Hewinson, R. Glyn; Neill, Sydney D.; Skuce, Robin A. Evaluation of
variable number tandem repeat (VNTR) loci in molecular typing of Mycobacterium
bovis isolates from
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/503320/description#description
NAL Call Number: SF601.V44
Descriptors: strain typing, Mycobacterium tuberculosis complex,
Mycobacterium bovis, exact tandem repeats (ETRs), mycobacterial interspersed
repetitive units (MIRUs), variable number tandem repeat (VNTR) loci,spoligotyping
using 47 field isolates, suggest a panel of VNTI markers for molecular epidemiological
studies.
Shah, D.H.;
Singh, S.K.; Rishendra Verma. Mycobacterium bovis-specific
500 bp DNA fragment is also present in the genome of Mycobacterium tuberculosis:
a growing evidence. In: C.T.N.F. Iskandar;
L. Hassan; G.K. Dhaliwal; R. Yusoff; A.R. Omar; M.A.K.G. Khan; (et-al). Animal
Health: A Breakpoint in Economic Development? The 11th International
Conference of the Association of Institutions for Tropical Veterinary Medicine
and 16th Veterinary Association Malaysia Congress, 23-27 August
2004, Petaling Jaya, Malaysia. 2004; 224-225.
ISBN: 9832871662
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis,
multiplex-PCR assay, 500bp DNA fragment, 185 bp PNCA product, human sputum samples.
Singh, Jitendra;
Joshi, Mohan Chandra; Bhatnagar, Rakesh. Cloning and expression of mycobacterial glutamine synthetase gene
in Escherichia coli. Biochemical and
Biophysical Research Communications. 2004; 317(2): 634-638. ISSN:
0006-291X
Descriptors: extracellular glutamine synthetase (GS) gene, prominent
proteins secreted by pathogenic mycobacteria, Mycobacterium tuberculosis,
Mycobacterium bovis, non-pathogenic Mycobacterium smegmatis
and Mycobacterium phlei do not secrete this protein, structure gene
amplified, fusion protein with hexahistidine residues in E. coli, solubilized
inclusion bodies, purified process for recombinant glutamine synthetase, first
report of cloning and expression of mycobacterial GS in E. coli.
Singh, S.K.;
Rishendra Verma; Shah, D.H. Molecular fingerprinting
of clinical isolates of Mycobacterium bovis and Mycobacterium tuberculosis
from
URL: http://www.vetsci.org/2004/pdf/331.pdf
Abstract: Forty mycobacterial strains comprising clinical Indian isolates
of Mycobacterium tuberculosis (28 field isolates +1H37 Rv)
and Mycobacterium bovis (10 field isolates +1 AN5) were subjected to restriction
fragment length polymorphism analysis (RFLP) using IS6110 and IS1081 probes.
Most of these strains originated from dairy cattle herd and human patients
from Indian Veterinary research Institute (IVRI) campus isolated from the
period of 1986 to 2000. Our study showed presence of 8 copies of IS6110 in
most of the M.tuberculosis (96.6%) strains irrespective of their origin with
the exception of one M.tuberculosis strain with presence of an extra copy
(3.4%). All M.bovis strains showed a single copy of IS6110 on the characteristic
1.9 kb restriction fragment. RFLP analysis with IS1081 invariably showed the
presence of 5 copies in all isolates of M.bovis and M.tuberculosis at the
same chromosomal location. Similarity of IS6110 RFLP fingerprints of M.tuberculosis
strains from animals and human suggested the possibility of dissemination
of single M.tuberculosis strain among animals as well as human. It was not
possible to discriminate within the isolates of either M.tuberculosis or M.bovis,
when IS1081 was used as target sequence. The IS6110 RFLP is a valuable tool
for disclosing transmission chain of M. tuberculosis and M. bovis among humans
as well as animals.
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis,
disease transmission between species, 40 mycobacterial strains, clinical
and field isolates, RFLP, IS6110 and IS1081 probes, dairy cattle herds, patients,
Indian Veterinary Research Institute campus, strains and species compared,
India.
Stermann, M.;
Sedlacek, L.; Maass, S.; Bange, F.C. A promoter mutation causes
differential nitrate reductase activity of Mycobacterium tuberculosis
and Mycobacterium bovis. Journal of Bacteriology.
2004; 186 (9): 2856-2861. ISSN: 0021-9193
URL: http://jb.asm.org/cgi/content/Abstractstract/186/9/2856
Abstract: The recent publication of the genome sequence of Mycobacterium
bovis showed >99.95% identity to M. tuberculosis. No genes
unique to M. bovis were found. Instead numerous single-nucleotide
polymorphisms (SNPs) were identified. This has led to the hypothesis that
differential gene expression due to SNPs might explain the differences between
the human and bovine tubercle bacilli. One phenotypic distinction between
M. tuberculosis and M. bovis is nitrate reduction, which not
only is an essential diagnostic tool but also contributes to mycobacterial
pathogenesis. We previously showed that narGHJI encodes a nitrate reductase
in both M. tuberculosis and M. bovis and that NarGHJI-mediated
nitrate reductase activity was substantially higher in the human tubercle
bacillus. In the present study we used a genetic approach to demonstrate
that an SNP within the promoter of the nitrate reductase gene cluster narGHJI
is responsible for the different nitrate reductase activity of M. tuberculosis
and M. bovis. This is the first example of an SNP that leads to differential
gene expression between the human and bovine tubercle bacilli..
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis,
pathogenesis, chromosomes, cosmids, cytosine, enzyme activity, gene expression,
genes, genome analysis, genomes, mutations, nitrite, promoters, thymine, no
genes unique to Mycobacterium bovis found, single nucleotide polymorphisms
identified, differential gene expression hypothesis, SNP in nitrate reductase
gene cluster nar GHJI different nitrate reductase between 2 pathogens.
Vesosky, B.;
Turner, O.C.; Turner, J.; Orme, I.M. Gamma interferon production
by bovine gammadelta T cells following stimulation with mycobacterial mycolylarabinogalactan
peptidoglycan. Infection and Immunity.
2004; 72 (8): 4612-4618. ISSN: 0019-9567
URL: http://iai.asm.org
NAL Call Number: QR1.I57
Abstract: A large percentage of lymphocytes in the blood of cattle
express the gammadelta T-cell receptor, but specific functions for these cells
have not yet been clearly defined. There is evidence, however, that human,
murine, and bovine gammadelta T-cells have a role in the immune response to
mycobacteria. This study investigated the ability of bovine gammadelta T-cells
to expand and produce gamma interferon (IFN-gamma) in response to stimulation
with mycobacterial products. Bovine gammadelta T-cells, isolated from the
peripheral blood of healthy cattle, expanded following in vitro stimulation
with live mycobacteria, mycobacterial crude cell wall extract, and Mycobacterium
bovis culture filtrate proteins. In addition, purified gammadelta T-cells,
co-cultured with purified monocytes and interleukin-2, consistently produced
significant amounts of IFN-gamma in response to mycobacterial cell wall. The
IFN-gamma-inducing component of the cell wall was further identified as a
proteolytically resistant, non-sodium dodecyl sulfate-soluble component of
the mycolylarabinogalactan peptidoglycan.
Descriptors: cattle, gamma interferon production, bovine gammadelta
T-cells, lymphocytes, ability to expand and produce IFN-gamma, stimulation,
live mycobacteria, mycobacterial crude cell wall extract, Mycobacterium
bovis culture filtrate, cell biochemistry.
Zink, A.R.;
Nerlich, A.G. Molecular strain identification
of the Mycobacterium tuberculosis complex in archival tissue samples.
Journal of Clinical Pathology (
URL: http://jcp.bmj.com/cgi/content/full/57/11/1185
Descriptors: identify human pathogenic mycobacteria, 49 archival tissue
sources, formalin fixed or paraffin wax embedded material, Mycobacterium
tuberculosis complex, Mycobacterium tuberculosis, Mycobacterium
bovis, Mycobacterium africanum, Mycobacterium microti, or
Mycobacterium canettii, and/or substrains, identifying individual infection
traits and superinfection by different strains, DNA analysis, IS6110
positive characterized by spoligotypin.
Zubrzycki, Igor Z. Analysis
of the products of genes encompassed by the theoretically predicted pathogenicity
islands of Mycobacterium tuberculosis and Mycobacterium bovis.
Proteins Structure Function and Bioinformatics. 2004; 54 (3): 563-568.
URL: http://www3.interscience.wiley.com/cgi-bin/fulltext/106567464/PDFSTART
Descriptors: Mycobacterium tuberculosisi, Mycobacterium bovis,
sequencing genomes, biology of pathogens, computational detection, anomalous
gene clusters, cross genomic comparisons, identified unique proteins of Mycobacterium
tuberculosis.
2003
Aranaz, Alicia; Cousins, Debby;
Mateos, Ana; Dominguez,-Lucas. Elevation of Mycobacterium tuberculosis
subsp. caprae Aranaz et al. 1999 to species rank as Mycobacterium
caprae comb. nov., sp. nov. International
Journal of Systematic and Evolutionary Microbiology. 2003; 53(6):
1785-1789. ISSN: 1466-5026
URL:
http://ijs.sgmjournals.org/cgi/content/full/53/6/1785?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&author1=aranaz&searchid=1&FIRSTINDEX=0&sortspec=relevance&resourcetype=HWCIT
NAL Call Number: QR1.I577
Descriptors: Mycobacterium tuberculosis complex, Spanish goat
isolates, Mycobacterium tuberculosis ssp. caprae, reclassification
to Mycobacterium caprae comb. nov., sp. nov, biochemical and epidemiological
parameters, combination of pncA, oxyR, katG and gyrA gene polymorphisms, special
nucleotide substitutions, isolated from other animals, cattle, wild boar,
pigs, genetic studies show older than Mycobacterium bovis, France,
Austria, Germany.
Buddle, B.M.; McCarthy, A.R.;
Ryan, T.J.; Pollock, J.M.; Vordermeier, H.M.; Hewinson, R.G.; Andersen, P.;
De Lisle, G.W. Use of mycobacterial peptides and recombinant
proteins for the diagnosis of bovine tuberculosis in skin test-positive cattle.
Veterinary Record. 2003; 153 (20): 615-620. ISSN:
0042-4900
URL: http://veterinaryrecord.bvapublications.com/
NAL Call Number: 41.8 V641
Descriptors: bovine tuberculosis, cattle, synthetic peptides, antigen
detection, skin lesions, skin tests, paratuberculosis, diagnostic techniques,
cross reaction, tuberculin, recombinant proteins, disease diagnosis, Mycobacterium
bovis, interferons, skin folds, vaccination, Mycobacterium avium
ssp. paratuberculosis, animal pathogenic bacteria.
Collins, Desmond M.; Kawakami,
R Pamela; Buddle, Bryce M.; Wards, Barry J.; De Lisle, Geoffrey W. Different
susceptibility of two animal species infected with isogenic mutants of Mycobacterium
bovis identifies phoT as having roles in tuberculosis virulence and phosphate
transport. Microbiology (Reading). 2003;
149 (11): 3203-3212. ISSN: 1350-0872
URL: http://mic.sgmjournals.org/contents-by-date.0.shtml
NAL Call Number: QR1.J65
Descriptors: Mycobacterium tuberculosis complex, Mycobacterium
bovis, ATCC35721, mutation, principal sigma factor gene, sigA, accessory
transcription factor WhiB3, M. bovis, Wag320, guinea pigs, brushtail
possum (Tricuosurus vulpecula), virulence restoring factor, phoT, role
in phosphate uptake at low phosphate concentrations, 2 point deletions, use
of different animal species.
Haddad, N.;
Masselot, M.; Durand, B. Molecular differentiation of Mycobacterium
bovis isolates. Review of main techniques and applications. Research in Veterinary Science. 2004: 76 (1): 1-18.
ISSN: 0034-5288
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/623070/description#description
NAL Call Number: 41.8 R312
Descriptors: Mycobacterium bovis, animal pathogenic bacteria
strains, strain differences, phylogeny, molecular genetics, transposons, repetitive
sequences, tandem repeat sequences, nucleotide sequences, literature reviews,
genetic polymorphism, epidemiology, population genetics, genetic markers,
pathogen identification, molecular markers.
Kurabachew,
M.; Enger, O.; Sandaa, R.A.; Eshetu-Lemma; Bjorvatn, B. Amplified ribosomal DNA restriction analysis in the differentiation
of related species of mycobacteria. Journal of Microbiological Methods. 2003; 55 (1): 83-90.
ISSN: 0167-7012
Descriptors: Mycobacterium bovis, Mycobacterium bovis
BCG strain, Mycobacterium tuberculosis, Mycobacterium africanum,
intra and inter species identification, amplified ribosomal DNA restriction
analysis, 16S and 23S rDNA, 16S-23SrDNA spacer, 121 isolates, 13 different
mybacterial species, restriction digestion, restriction enzymes, CfoI, HaeIII,
RsaI, MspI, TaqI, method to recognize strains of M. tubuculosis complex
and others.
Lewin, A.; Freytag, B.; Meister,
B.; Sharbati Tehrani, S.; Schaefer, H.; Appel, B. Use of a quantitative TaqMan-PCR for the fast quantification of
mycobacteria in broth culture, eukaryotic cell culture and tissue.
Journal of Veterinary Medicine Series B.
2003; 50 (10): 505-509. ISSN: 0931-1793
URL: http://www.blackwellpublishing.com/journal.asp?ref=1863-1959&site=1
Descriptors: Mycobacterium tuberculosis, Mycobacterium bovis
BCG, quantification, in vitro samples, in vivo samples, growth curves,
broth cultures, quantitative TaqMan PCR, multiplication within eukaryotic
cells, load in tissue before colony counts.
Lis, Henryk. Wystepowanie i zwalczanie gruzlicy bydla w niektorych panstwach
Unii Europejskiej i w Polsce. [Incidence and
eradication of bovine tuberculosis in some EU countries and in
Descriptors: Mycobacterium bovis, animal pathogen, farm animals,
disease distribution, outbreaks can still occur in Poland, present in other
EU countries, France, Greece, Ireland, Italy, Portugal, Spain.
Marano, Nina;
Pappaioanou, Marguerite. Historical, new, and reemerging links
between human and animal health. Emerging Infectious Diseases. 2004; 10 (12): 2065-2066.
ISSN: 1080-6040
URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?linkbar=plain&db=journals&term=1080-6040
NAL Call Number: RA648.5.E46
Descriptors: human and animal disease interactions, zoonotic disease,
transmission of disease between species, historical information, Hantavirus,
Salmonella Newport, West Nile virus, Mybacterium bovis-tuberculosis,
Nipah virus, infectious diseases, epidemiology, prevention and control, global
trade, human behaviors, international travel of humans and animals, rapid
microbial adaptation, wildlife reservoirs.
Maslow, Joel N.; Irani, Vida R.;
Lee, Sun Hwa; Eckstein, Torsten M.; Inamine, Julia M.; Belisle, John T. Biosynthetic
specificity of the rhamnosyltransferase gene of Mycobacterium avium
serovar 2 as determined by allelic exchange mutagenesis. Microbiology
(Reading). 2003; 149 (11): 3193-3202. ISSN: 1350-0872
URL: http://mic.sgmjournals.org/contents-by-date.0.shtml
NAL Call Number: QR1.J65
Descriptors: Mycobacterium bovis serovar strain 2 TMC724 derived
via a plasmid, Mycobacterium smegnatis, rhamnosyltransferase, rtfA
gene, catalyses addion of rhamnose to 6-deoxytalose of serover 2-specific
glycopeptidolipid, alaninol, lipipeptide, system of allelic exchange for M.
avium as a tool for future genetic studies.
Quesniaux, V.; Fremond, C.; Jacobs,
M.; Shreemanta Parida; Nicolle, D.; Yeremeev, V.; Bihl, F.; Erard, F.; Botha,
T.; Drennan, M.; Soler, M.N.; Bert, M. le; Schnyder, B.; Ryffel, B. Toll-like
receptor pathways in the immune responses to mycobacteria. Microbes
and Infection. 2004; 6 (10): 946-959.
Descriptors: mice, laboratory animals, disease model, Mycobacterium
avium, Mycobacterium bovis, Mycobacterium chelonae, Mycobacterium
kansasii, Mycobacterium smegmatis, Mycobacterium tuberculosis,
bacterial antigens, bacterial proteins, biochemical receptors, cell cultures,
disease models, experimental infections, immune response, in vitro, ligands,
mycobacterial diseases, literature reviews tuberculosis toll-like receptors.
Ramarokoto, H.; Andrianasolo,
D.; Rasolonavalona, T.; Ramaroson, F.; Razafitsiarovana, I.; Vincent, V.;
Ratsimba, L.; Rasolofo Razanamparany, V. Un
cas de tuberculose pulmonaire a Mycobacterium bovis multiresistant
a
Descriptors: Mycobacterium bovis, case study, animal to human
transfer, multi-drug resistant strain, Malagasy citizen.
Sinha,
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/506058/description#description
NAL Call Number: QR1.F44
Abstract: Probing protein extracts from exponentially growing and stationary
phase cultures of Mycobacterium bovis BCG with anti-phospho amino acid
antibodies revealed a 31-kDa anti-phospho threonine antibody-reactive protein
specific to growing culture. The corresponding protein was purified via two-dimensional
gel electrophoresis and identified via mass spectrometry to be malonyl coenzyme
A:acyl carrier protein transacylase (MCAT), a component
of the fatty acid biosynthetic pathway. MCAT tagged with histidine reacted
with anti-phospho threonine antibody and was positive in an in-gel chemical
assay for phospho proteins. Analysis of the growth phase dependence of MCAT-His
phosphorylation and protein levels showed that phosphorylated MCAT-His can
be detected only in growing culture. In contrast, MCAT-His protein level
was growth phase-independent. These results suggest that MCAT may be a substrate
of a protein kinase and phosphatase, and that aspects of fatty acid synthesis
in tubercle bacilli are regulated by protein phosphorylation.
Descriptors: animal pathogenic bacteria, Mycobacterium bovis,
phosphorylated MCAT-His, MCAT-His protein levels, fatty acid synthesis.
Smith, N.H.; Dale, J.; Inwald,
J.; Palmer, S.; Gordon, S.V.; Hewinson, R.G.; Smith, J.M. The population
structure of Mycobacterium bovis in
NAL Call Number: 500 N21P
URL: http://www.pnas.org/cgi/content/full/100/25/15271
Abstract: We have analyzed 11,500 isolates of Mycobacterium bovis
(the cause of tuberculosis in cattle and other mammals) isolated
in
Descriptors: Mycobacterium bovis, animal pathogenic bacteria,
population structure, mini-satellite repeats, evolution, geographical distribution,
bovine tuberculosis, spoligoypes, clones with recent common ancestor, distribution
of different genotypes, clonal expansion,England, Wales, Scotland.
Sreedevi, B.;
Krishnappa, G. Pathogenesis of Mycobacterium tuberculosis
isolated from cattle. Indian Journal of Comparative
Microbiology, Immunology and Infectious Diseases. 2003; 24 (1):
59-62. ISSN: 0970-9320
Descriptors: cattle, Mycobacterium, Mycobacterium avium,
Mycobacterium bovis, Mycobacterium tuberculosis in cattle, different
mycobacterial cultures, bovine macrophage cell cultures, NBT dye reduction
test, disease transmission, levels of pathogenisity, phagocytosis, cattle
as host organisms.
Taylor, S.J.;
Ahonen, L.J.; De Leij, F.A.A.M.; Dale, J.W. Infection of Acanthamoeba castellanii with Mycobacterium
bovis and M. bovis BCG and survival of M. bovis within the
amoebae. Applied
and Environmental Microbiology. 2003; 69 (7): 4316-4319. ISSN:
0099-2240.
URL: http://www.pubmedcentral.nih.gov/tocrender.fcgi?action=archive&journal=83
NAL Call Number: 448.3 Ap5
Abstract: Survival of Mycobacterium bovis after ingestion by
protozoa would provide an environmental reservoir for infection of cattle.
We have shown that M. bovis survived ingestion by Acanthamoeba
castellanii. In contrast, two strains of M. bovis BCG did not
survive well within Acanthamoeba.
Descriptors: possible environmental reservoir, Mycobacterium bovis,
Mycobacterium bovis BCG, animal pathogenic bacteria, ingestion by Acanthamoeba
castellanii, pathogen survival, disease reservoirs, bovine tuberculosis,
soil fauna, cattle pastures.
Varela, E.; Paez, A. Montano,
L.F.; Masse, F: Isolation and characterization of Mycobacterium bovis
19 kDa native protein distinct from MPB 70/80. Molecular
and Cellular Proteomics. 2003; 2 (9): 967. ISSN: 1535-9476.
Note: Meeting abstract. Meeting: HUPO (Human Proteomics Organisation) 2nd
Annual and IUBMB (International Union of Biochemistry and Molecular Biology)
XIX World Congress,
Descriptors: Mycobacterium bovis, animal pathogen, 19-kDa-native
protein characterization, isolation, MPB70-80 isoelectric focusing, electrophoretic
techniques.
Zhang, XiYue;
Wu, YanGong; Wang, ZhiLiang; Xu, PeiLian; Zhao, YunLing. Study
on ELISA for detecting bovine tuberculosis. Chinese
Journal of Animal Quarantine. 2004; 21 (7): 21-22. ISSN: 1005-944x.
Note: In Chinese with an English summary.
Descriptors: Mycobacterium bovis, diagnosis, improved classical
ELISA, sodium azide as protective agent, PPD coated plates, cattle serum diluent,
TMB as substrate, good specificity.
Zhang, XiYue; Wang, JunWei; Gao,
YunHang; He, ZhaoYang. Study on detection of tuberculosis antibodies in
serum of cattle by Dot-IGSS. Journal of
Descriptors: cattle, Mycobacterium bovis, detection of serum
antibodies, Dot IGSS (Dot-immunogold silver staining, diagnostic technique.
2002
Gutierrez-Pabello,
J.A.; McMurray, D.N.;
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: Bovine macrophages underwent apoptosis as a result of infection
with a Mycobacterium bovis field strain. Macrophages infected with
a multiplicity of infection (MOI) of 25:1 developed chromatin condensation
and DNA fragmentation at 4 h and 8 h, respectively, whereas changes in chromatin
condensation induced by MOIs of 10:1 and 1:1 required more time and had a
reduced number of apoptotic cells. Not only infected macrophages underwent
apoptosis, but also uninfected bystander macrophages became apoptotic. Increased
differential expression of thymosin beta-10 was identified in M. bovis-infected
bovine macrophages by differential display reverse transcriptase PCR. Phagocytosis
of latex beads had no effect on the expression of thymosin beta-10, whereas
bacterial suspensions upregulated thymosin beta-10 expression, suggesting
that M. bovis or mycobacterial products are essential in the process.
Heat-inactivated M. bovis induced a slight increase in thymosin beta-10
mRNA, whereas live virulent and attenuated M. bovis organisms increased
the gene expression almost twofold. A mouse macrophage cell line (RAW 264.7)
overexpressing the bovine thymosin beta-10 transgene had spontaneous apoptosis
at a higher rate (66.5%) than parental cells (4.7%) or RAW cells harboring
the empty vector (22.8%). The apoptotic rates of the overexpressing cells
were significantly higher when compared with both the empty vector transfected
(P < 0.01) and parental cells (P < 0.001). Our evidence suggests that
upregulation of thymosin beta-10 in M. bovis-infected macrophages is
linked with increased cell death due to apoptosis.
Descriptors: molecular sequence data, cattle, messenger RNA, complementary
DNA, nucleotide sequences.
Jesenska,
A.; Bartos, M.; Czernekova, V.; Rychlik, I.; Pavlik, I.; Damborsky, J. Cloning
and expression of the haloalkane dehalogenase gene dhmA from Mycobacterium
avium N85 and preliminary characterization of DhmA. Applied
and Environmental Microbiology. 2002. 68 (8) 3724-3730.
NAL
Call Number: 448.3 Ap5
Descriptors: carbon, catalysts, cleavage, DNA cloning, enzyme
activity, gene expression, genetics, genomes, halogens, Mycobacterium avium,
Mycobacterium tuberculosis, Mycobacterium bovis, Photobacterium, Xylella fastidiosa.
Kwong,
L.S.; Hope, J.C.; Thom, M.L.; Sopp, P.; Duggan, S.; Bembridge, G.P.; Howard,
C.J. Development of an ELISA for bovine IL-10. Veterinary
Immunology and Immunopathology. 2002; 85 (3/4): 213-223. ISSN:
0165-2427
URL: http://www.elsevier.com/wps/find/journalabstracting.cws_home/503319/abstracting
NAL Call Number: SF757.2.V38
Abstract: The objective of the study was to develop an assay for bovine
IL-10 that could be applied to analyses of immune responses and advance understanding
of a variety of diseases of cattle. Recombinant bovine IL-10 (rbo IL-10)
was transiently expressed in Cos-7 cells and shown to inhibit the synthesis
of IFNgamma by bovine cells stimulated with antigen in vitro. Mice were immunised
with a plasmid containing a cDNA insert encoding rbo IL-10 and inoculated
with rbo IL-10. A number of monoclonal antibodies (mab) were generated that
reacted with rbo IL-10 in an ELISA. Some of these mab neutralised the ability
of rbo IL-10 to inhibit IFNgamma synthesis by antigen-stimulated bovine cells.
A pair of mabs was identified that together could be used to detect both
recombinant and natural bovine IL-10 present in supernatant of PBMC stimulated
with ConA. A luminescent detection method was applied to the ELISA making
it more sensitive. Using this method native IL-10 was detected in supernatants
of PBMC, diluted blood and undiluted blood from cattle immunised with Mycobacterium
bovis BCG or ovalbumin and incubated in vitro with antigen indicating
the applicability of the assay to a number of in vitro culture systems.
Descriptors: cattle, interleukin 10, ELISA, monoclonal antibodies,
interferon, recombinant DNA, complementary DNA, protein synthesis, inhibition.
Lysenko, A.P.;
Krasnikova, E.L.; Poloz, A.I. Morphological characteristics
of Mycobacterium species cultured in new VKG medium.
Veterinarnaya Nauka Proizvodstvu. 2002; (36): 69-75. Note:
In Russian.
Descriptors: Mycobacterium bovis, Mycobacterium fortuitum,
Mycobacterium tuberculosis, bacteriology, culture media effects, VKG
medium.
Milian-Suazo, F.; Banda-Ruiz, V.; Ramirez-Casillas,
C.; Arriaga-Diaz, C. Genotyping of Mycobacterium bovis by geographic
location within
NAL
Call Number: SF601 P7
Descriptors: spoligotyping, differentiate 62 Mycobacterium
bovis isolates, dairy cattle, genetic differences, detection of infection
sources.
Niemann,
S.; Richter, E.; Rusch-Gerdes, S. Biochemical and genetic evidence for
the transfer of Mycobacterium tuberculosis subsp. caprae
Aranaz et al. 1999 to the species Mycobacterium bovis Karlson
and Lessel 1970 (Approved Lists 1980) as Mycobacterium bovis subsp.
caprae comb. nov. International
Journal of Systematic and Evolutionary Microbiology. Mar 2002.
52 (pt.2) 433-436. ISSN: 1466-5026.
URL:
http://ijs.sgmjournals.org/
NAL Call Number: QR1.I577
Descriptors: new combination, new subspecies, descriptions,
taxonomy, chemotaxonomy, Mycobacterium bovis ssp. caprae.
Nishimori,
K.; Uchida, I.; Tanaka, K.; Nishimori, T.; Imai, K.; Kashiwazaki, Y.; Murata,
N.; Jinma, K. Molecular epidemiological manual for Mycobacterium
tuberculosis complex and Mycobacterium avium using VNTR (Variable
Numbers of Tandem Repeats) typing. Bulletin
of the National Institute of Animal Health. 2002, No.109, 25-32.
ISSN: 1347-2542 Note: In Japanese with an English summary.
NAL
Call Number: 41.9 T572
Descriptors:
bacterial typing, Mycobacterium avium, Mycobacterium tuberculosis,
molecular epidemiology, phylogeny.
Poloz, A.I.;
Lysenko, A.P.; Krasnikova, E.L. [Differential diagnosis of Mycobacterium
tuberculosis and atypical Mycobacterium strains using immunofluorescent
microscopy.] Veterinarnaya Nauka Proizvodstvu. 2002; (36): 141-144.
Note: In Russian.
Descriptors: Mycobacterium bovis, Mycobacterium tuberculosis,
differential diagnosis, immunofluorescence, diagnostic techniques, strain
differences.
Roring,
S.; Scott, A.; Brittain, D.; Walker, I.; Hewinson, G.; Neill, S.; Skuce, R.
Development of variable-number tandem repeat typing of Mycobacterium
bovis: comparison of results with those obtained by using existing exact
tandem repeats and spoligotyping. Journal of Clinical
Microbiology. 2002; 40 (6): 2126-2133. ISSN: 0095-1137
URL: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1489394
NAL Call Number: QR46.J6
Descriptors: Mycobacterium bovis, repetitive DNA.
Shah, D.H.; Verma, R.; Bakshi, C.S.; Singh, R.K. A
multiplex-PCR for the differentiation of Mycobacterium bovis and Mycobacterium
tuberculosis. FEMS Microbiology Letters. Aug
27, 2002.
214 (1) 39-43. ISSN: 0378-1097
NAL
Call Number: QR1.F44
Abstract: A multiplex-polymerase
chain reaction (PCR) assay based on one-step amplification and detection of
two different mycobacterial genomic fragments was designed for differentiation
of Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide
primers were chosen from a 500-bp genomic fragment which is well conserved
in M. bovis and the pncA gene (based on M. tuberculosis-specific
nucleotide polymorphism, a cytosine residue at position 169), specific for
M. tuberculosis. The multiplex-PCR allowed detection of a single product
of 500 bp in M. bovis isolates while M. tuberculosis isolates
generated a single product of 185 bp, with or without an additional product
of 500 bp. None of the atypical mycobacterial isolates revealed any amplification
products. The method was found to be highly specific and could detect as little
as 20 pg of pure DNA. This multiplex-PCR assay, based on the 500-bp fragment
and the pncA gene, may be very useful for the rapid and specific differentiation
of these two closely related mycobacteria and easy to use in medical and veterinary
microbiological laboratories.
Descriptors: polymerase chain reaction, multiplex polymerase
chain reaction, species differentiation, Mycobacterium bovis, Mycobacterium
tuberculosis, rapid testing method.
Shyam Unniraman; Monalisa Chatterji; Valakunja
Nagaraja.
DNA gyrase genes in Mycobacterium tuberculosis: a single operon
driven by multiple promoters. Journal of Bacteriology.
2002. 184 (19) 5449-5456. ISSN: 0021-9193
NAL Call Number: 448.3 J822
Descriptors: auto-regulation, genes, genomes, isomerases, molecular
genetics, nucleotide sequences, operons, promoters, transcription, Mycobacterium
tuberculosis, DNA topoisomerase (ATP hydrolysing).
Roring,
S.; Scott, A.; Brittain, D.; Walker, I.; Hewinson, G.; Neill, S.; Skuce, R.
Development of variable-number tandem repeat typing of Mycobacterium
bovis: comparison of results with those obtained by using existing exact
tandem repeats and spoligotyping. Journal of Clinical
Microbiology. 2002. 40 (6) 2126-2133.
NAL Call Number: QR46.J6
Descriptors: fingerprinting, Mybacterium tuberculosis
complex, RFLP typing, alleles, genes, genetic polymorphism, loci, molecular
genetics, nucleotide sequences, repetitive DNA, restriction fragment length
polymorphism, Mycobacterium bovis.
Skuce,
R.A.; McCorry, T.P.; McCarroll, J.F.; Roring, S.M.M.; Scott, A.N.; Brittain,
D.; Hughes, S.L.; Hewinson, R.G.; Neill, S.D. Discrimination of Mycobacterium
tuberculosis complex bacteria using novel VNTR-PCR targets. Microbiology. Feb 2002. 148 (pt.2)
519-528. ISSN: 1350-0872
NAL Call Number: QR1.J64
Descriptors: Mycobacterium bovis, bovine tuberculosis,
variable number tandem repeats, polymerase chain reaction, spoligotyping.
Smits, T.H.M.; Balada, S.B.; Witholt, B.; van Beilen,
J.B. Functional analysis of alkane hydroxylases from gram-negative and
gram-positive bacteria. Journal of Bacteriology. 2002. 184 (6) 1733-1742.
NAL Call Number: 448.3 J82
Descriptors: alkanes, amino acid sequences, enzyme activity,
gene expression, genetic analysis, gram negative bacteria, gram positive bacteria,
oxidoreductases, oxygenases, soil bacteria, Acinetobacter, Escherichia coli,
Mycobacterium tuberculosis, Pseudomonas aeruginosa, Pseudomonas
fluorescens, Pseudomonas putida, Alcanivorax borkumensis,
Prauserella rugosa, rubredoxin NAD+-reductase.
Ucko, M.; Colorni, A.; Kvitt, H.; Diamant, A.;
Zlotkin, A.; Knibb, W.R. Strain variation in Mycobacterium marinum fish
isolates. Applied and Environmental Microbiology. ISSN: 0099-2240
Nov 2002, 7(11) 6114-6120.
NAL
Call Number: 448.3 AP5
Descriptors:
fish pathogen, Mycobacterium marinum, genetics, strain variations.
Welsh,
M.D.; Kennedy, H.E.; Smyth, A.J.; Girvin, R.M.; Andersen, P.; Pollock, J.M.
Responses of bovine WC1+ gammadelta T cells to protein
and nonprotein antigens of Mycobacterium bovis. Infectious Immunity. 2002; 70 (11): 6114-6120.
ISSN: 0019-9567.
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: WC1(+) gammadelta T cells of Mycobacterium bovis-infected
cattle are highly responsive to M. bovis sonic extract (MBSE). In
mycobacterial infections of other species, gammadelta T cells have been shown
to respond to protein and nonprotein antigens, but the bovine WC1(+)
gammadelta T-cell antigenic targets within MBSE require further definition
in terms of the dominance of protein versus nonprotein components. The present
study sought to characterize the WC1(+) gammadelta T-cell antigenic targets, together with the
role of interleukin-2 (IL-2), in the context of M. bovis infection. This
was achieved by testing crude and defined antigens to assess protein versus
nonprotein recognition by WC1(+) gammadelta T cells
in comparison with CD4(+) alpha beta T cells. Both cell types proliferated
strongly in response to MBSE, with CD4(+) T cells
being the major producers of gamma interferon (IFN-gamma). However, enzymatic
digestion of the protein in MBSE removed its ability to stimulate CD4(+)
T-cell responses, whereas some WC1(+) gammadelta T-cell proliferation remained.
The most antigenic protein inducing proliferation and IFN-gamma secretion
in WC1(+) gammadelta T-cell cultures was found to
be ESAT-6, which is a potential novel diagnostic reagent and vaccine candidate.
In addition, WC1(+) gammadelta T-cell proliferation
was observed in response to stimulation with prenyl pyrophosphate antigens
(isopentenyl pyrophosphate and monomethyl phosphate). High levels of cellular
activation (CD25 expression) resulted from MBSE stimulation of WC1(+) gammadelta T cells from infected animals. A similar
degree of activation was induced by IL-2 alone, but for WC1(+)
gammadelta T-cell division IL-2 was found to act only as a costimulatory signal,
enhancing antigen-driven responses. Overall, the data indicate that protein
antigens are important stimulators of WC1(+) gammadelta
T-cell proliferation and IFN-gamma secretion in M. bovis infection,
with nonprotein antigens inducing significant proliferation. These findings
have important implications for diagnostic and vaccine development.
Descriptors: T lymphocytes, the WC1(+) gammadelta
T-cell antigenic targets, bacterial antigens, lymphocyte transformation.
2001
Arraiz,
N.; Takiff, H. Analisis de ARNm de un factor de supervivencia en micobacterias. [mRNA
analysis of a survival factor in mycobacteria.] Kasmera. 2001. 29 (1) 65-82. Note: In Spanish with an
English summary.
Descriptors: Mycobacterium tuberculosis, Mycobacterisum bovis
BCG, the sigma ECF factor SuoM, SuoM-lac Z, transcriptional fusion reporters,
beta-glactosidase activity, heat shock effects, cell growth, survival functions.
Caimi, K.; Romano, M.I.; Alito, A.; Zumarraga,
M.; Bigi, F.; Cataldi, A. Sequence analysis of the direct repeat region
in Mycobacterium bovis. Journal
of Clinical Microbiology.
2001. 39 (3) 1067-1072.
NAL
Call Number: QR46.J6
Descriptors: DNA sequencing, Mycobacterium bovis, nucleotide
sequences, cattle tuberculosis.
Chambers,
M.A.; Williams, A.; Gavier-Widen, D.; Whelan, A.; Hughes, C.; Hall, G.; Lever,
M.S.; Marsh, P.D.; Hewinson, R.G. A guinea pig model
of low-dose Mycobacterium bovis aerogenic infection. Veterinary
Microbiology. 2001; 80 (3): 213-226.
ISSN: 0378-1135.
URL: http://www.elsevier.com/wps/find/journaldescription.cws_home/503320/description#description
NAL Call Number: SF601.V44
Abstract: In order to develop a model of Mycobacterium bovis
infection with pathogenetical relevance, a modified version of the
Descriptors: guinea pigs, Mycobacterium bovis, experimental
infections, animal models, disease models, inoculum density, pathogenesis,
airborne infection, intramuscular injection, application methods, lungs, spleen,
colonization, antigens, dosage effects.
Clifton-Hadley,
Richard S.; Sauter Louis, Carola M.; Lugton, Ian W.; Jackson, Ronald; Durr,
Peter A.; Wilesmith, John W. Mycobacterial diseases.
Mycobacterium bovis infections. In:
Elizabeth S. Williams; Ian K. Barker (editors). Infectious
diseases of wild mammals. Third edition.
Descriptors: Mycobacterium, Mycobacterium bovis, ferrets
(Mustela putorius furo), badgers (Meles meles), brush tailed possoms (Trichosurus vulpecula) wildlife
as disease reservoirs, domestic animals, cattle, zoonotic diseases.
Collins, D.M.; Ellner, J.J. (ed.); Brennan, P.J.
(ed.); Young, D. Virulence factors of Mycobacterium bovis. Tuberculosis.
2001. 81 (1-2) 97-102. Note: Third international conference on Mycobacterium
bovis, M. bovis 2000, Cambridge,
Descriptors: genes, mutant strains, Mycobacterium bovis,
Mycobacterium tuberculosis, disease screening for virulence, cattle.
Dannenberg,
A.M. Jr.; Ellner, J.J. (ed.); Brennan, P.J. (ed.); Young, D. Pathogenesis
of pulmonary Mycobacterium bovis infection: basic principles established
by the rabbit model. Tuberculosis. 2001.
81 (1-2) 87-96. Note: Third international conference on Mycobacterium
bovis, M. bovis 2000, Cambridge,
Descriptors: pathogenesis, rabbit disease model, Mycobacterium
bovis strains, species differences, antibodies, antigens, cell mediated
immunity, immune response, chemokines, cytokines, delayed type hypersensitivity,
disease control, experimental infections, laboratory animals, lungs, macrophage
activation, respiratory diseases, T lymphocytes, tuberculosis.
De
Mendonca-Lima, L.; Picardeau, M.; Raynaud, C.; Rauzier, J.; de la Salmoniere,
Y.O.G.; Barker, L.; Bigi, F.; Cataldi, A.; Gicquel, B.; Reyrat, J.M. Erp,
an extracellular protein family specific to mycobacteria. Microbiology.
2001. 147 (8) 2315-2320.
NAL Call Number: QR1.J64
Descriptors: exported repeated protein, Mycobacterium,
Mycobacterium tuberculosis, Mycobacterium smegmatis, ubiquitous
extracellular protein, genetic conservation.
Gordon,
S.V.; Eiglmeier, K.; Garnier, T.; Brosch, R.; Parkhill, J.; Barrell, B.; Cole,
S.T.; Hewinson, R.G.; Ellner, J.J. (ed.); Brennan, P.J. (ed.); Young, D. Genomics
of Mycobacterium bovis. Tuberculosis.
2001. 81 (1-2) 157-163. Note: Third international conference on Mycobacterium
bovis, M. bovis 2000, Cambridge,
Descriptors: genetic variation, genetics, genomes, nucleotide
sequences, Mycobacterium bovis, BCG strain, Mycobacterium tuberculosis,
pathogenicity, phenotypes, proteins, virulence, reviews.
Haddad,
N.; Durand, B. Interet et limites des differentes techniques de caracterisation des
isolats. Exemple de la tuberculose.
[Interest and limits of different technics for the study of strains: Example
of tuberculosis.] Epidemiologie et
Sante Animale. 2001. No. 39, 43-57. Note: In French with an English summary.
Descriptors: epidemiology, molecular biology, tuberculosis,
Mycobacterium.
Haddad,
N.; Ostyn, A.; Karoul, C.; Masselot, M.; Thorel, M.F.; Hughes, S.L.; Inwald,
J.; Hewinson, R.G.; Durand, B. Spoligotype diversity of Mycobacterium
bovis strains isolated in France from 1979 to 2000. Journal
of Clinical Microbiology. Oct 2001. 39 (10) 3623-3632.
ISSN: 0095-1137
NAL Call Number: QR46.J6
Descriptors: cattle tuberculosis, strains, genetic diversity,
Mycobacterium.
Hotter, G.S.; Wilson, T.; Collins, D.M. Identification
of a cadmium-induced gene in Mycobacterium bovis and Mycobacterium
tuberculosis. FEMS Microbiology Letters. June
25, 2001. 200 (2) 151-155. ISSN: 0378-1097
NAL Call Number: QR1.F44
Abstract: A 17-kDa protein (CadI) was induced by cadmium
in Mycobacterium bovis and Mycobacterium tuberculosis. Comparison
of the N-terminal sequence from M. bovis CadI with the annotated M.
tuberculosis genome database identified Rv2641 as the encoding gene. Long
and short promoter fragments from M. bovis cadI were fused to the lacZ
reporter gene in pYUB76. Only the long fragment directed cadmium-inducible
activity when electroporated into M. bovis. The CadI promoter has potential
for both constitutive and inducible expression studies in M. bovis
and M. tuberculosis.
Descriptors: Mycobacterium bovis,
Mycobacterium tuberculosis, CadI,
cadmium, genetics, promoter fragments, lacZ reporter
gene, expression studies.
Inglis,
N.F.; Stevenson, K.; Davies, R.C.; Heaslip, D.G.; Sharp, J.M. Unique expression
of a highly conserved mycobacterial gene in IS901+ Mycobacterium avium.
Microbiology. June 2001. 147 (pt. 6) 1557-1564.
ISSN: 1350-0872
NAL Call Number: QR1.J64
Abstract: Expression of a gene encoding a novel protein antigen
of 40 kDa (p40) was detected in IS901+ strains of Mycobacterium avium,
but not in any other species or subspecies of Mycobacterium tested,
including IS901- M. avium and the other members of the M. avium
complex. Although Southern hybridization revealed that the p40 gene is
widely distributed within the genus, expression of the antigen could not be
detected on Western blots of mycobacterial cell lysates. Nucleotide sequence
analysis of the cloned p40 gene, and a database search, revealed high levels
of sequence identity with a homologous gene in IS901- M. avium, M.
avium subsp. paratuberculosis, Mycobacterium bovis, Mycobacterium leprae,
Mycobacterium smegmatis and Mycobacterium tuberculosis. Further
analysis of upstream sequences identified a putative promoter region. The
p40 gene is the first example of a gene that is widely distributed within
the genus Mycobacterium but expressed only
in association with the presence of a genomic insertion element, in this case
IS901, in strains of M. avium isolated from birds and domestic livestock.
Descriptors: Mycobacterium
avium, Mycobacterium avium subsp. paratuberculosis,
Mycobacterium bovis, Mycobacterium leprae, Mycobacterium smegmatis, Mycobacterium
tuberculosis, novel protein antigen, Western blot method.
Kato,
Maeda M.; Rhee, J.T.; Gingeras, T.R.; Salamon, H.; Drenkow, J.; Smittipat,
N.; Small, P.M. Comparing genomes within the species Mycobacterium tuberculosis.
Genome Research. 2001. 11 (4) 547-554.
NAL Call Number: QP606 D46P34
Descriptors: genetic variability, natural populations of Mycobacterium
tuberculosis, evolution, pathogenesis, small scale genomic deletions,
19 isolates.
Kauppinen,
J.; Hintikka, E.L.; Iivanainen, E.; Katila, M.L. PCR-based typing of Mycobacterium
avium isolates in an epidemic among farmed lesser white-fronted geese
(Anser erythropus). Veterinary Microbiology. July 3, 2001. 81 (1) 41-50.
ISSN: 0378-1135
NAL Call Number: SF601.V44
Abstract: Mycobacterium avium is an important veterinary
pathogen causing avian tuberculosis in birds. The aim of the study was to
evaluate the genetic relatedness in M. avium isolates from deep tissues
of farmed lesser white-fronted geese with avian tuberculosis and in samples
from the farm environment. The strains were analyzed by two PCR-based typing
methods, inverted repeat (IR) typing and random amplified polymorphic DNA
(RAPD) analysis. The primers for the inverted repeats of the insertion sequences
IS1245 and IS1311 were used in IR typing, and the RAPD analysis was performed
with six primers. Seven of the nine avian strains yielded an identical pattern
in the IR typing, but they could be divided into two groups in the RAPD analysis.
The remaining two bird isolates had an identical IR pattern (IR cluster II)
which they shared with two environmental isolates. However, the RAPD analysis
revealed that these environmental isolates had a RAPD pattern (RAPD cluster
VI) distinct and different from either of the bird isolates (RAPD clusters
II and IV). In all, four M. avium strains were verified as being inducers
of avian tuberculosis in birds, and all were distinct from the three environmental
strains identified. Thus, the results did not confirm the preliminary idea
that a single strain had caused the epidemic. The polymorphism among M.
avium strains highlighted the great biodiversity among an M. avium
population even in a limited environmental setting during a short time span,
and indicated the high susceptibility to avian tuberculosis of lesser white-fronted
geese.
Descriptors: Anser erythropus, geese, Mycobacterium
avium, polymerase chain reaction, genotypes, identification, strain differences,
genetic distance, random amplified polymorphic DNA, nucleotide sequences,
epidemics, genetic diversity, susceptibility.
Koul,
A.; Choidas, A.; Tyagi, A.K.; Drlica, K.; Singh, Y.; Ullrich, A. Serine/threonine
protein kinases PknF and PknG of Mycobacterium tuberculosis: characterization
and localization. Microbiology. 2001. 147 (8) 2307-2314.
NAL Call Number: QR1.J64
Descriptors: serine protein kinases, threonine protein kinases,
genes, phosphorylation, trans-membrane proteins, Mycobacterium bovis,
Mycobacterium smegmatis, Mycobacterium tuberculosis, pathogenesis,
cellular signaling network.
Li,
MingShi; Monahan, I.M.; Waddell, S.J.; Mangan, J.A.; Martin, S.L.; Everett,
M.J.; Butcher, P.D. cDNA-RNA subtractive hybridization reveals increased
expression of mycocerosic acid synthase in intracellular Mycobacterium
bovis BCG. Microbiology. 2001.
147 (8) 2293-2305.
NAL Call Number: QR1.J64
Descriptors: gene expression, genes, ligases, macrophages, molecular
genetics, Mycobacterium bovis, mycocerosic acids.
Njanpop-Lafourcade,
B.M.; Inwald, J.; Ostyn, A.; Durand, B.; Hughes, S.; Thorel, M.F.; Hewinson,
G.; Haddad, N. Molecular typing of Mycobacterium bovis isolates
from Cameroon. Journal of Clinical Microbiology.
Jan 2001. 39 (1) 222-227. ISSN: 0095-1137
NAL Call Number: QR46.J6
Descriptors: molecular epidemiology, 75 Mycobacterium bovis
isolates, spoligotyping, pulsed-field gel electrophoresis, PFGE, restriction
fragment length polymorphism, RFLP, probe IS6110-RHS, homogeneity, geographical
mapping of strains, cattle tuberculosis, biochemical techniques, control of
disease, cattle,
Olsen,
I.; Tryland, M.; Wiker, H.G.; Reitan, L.J. AhpC, AhpD, and a secreted 14-kilodalton
antigen from Mycobacterium avium subsp. paratuberculosis
distinguish between paratuberculosis and bovine tuberculosis in an enzyme-linked
immunosorbent assay. Clinical and Diagnostic Laboratory
Immunology. 2001. 8 (4) 797-801.
Descriptors: Mycobacterium avium ssp. paratuberculosis,
Mycobacterium bovis, experimental infection, ELISA, species identification,
antibodies, differential diagnostic techniques, antibody testing, sera.
Rastogi, N.; Legrand, E.; Sola, C. The mycobacteria: an
introduction to nomenclature and pathogenesis. Revue Scientifique et
Technique Office International des Epizooties. 2001. 20 (1) 21-54. Note:
In English with French and Spanish summaries.
NAL
Call Number: SF781 R4
Descriptors: nomenclature, diagnosis, macrophages, mycobacterial
diseases, pathogenesis, phylogeny, taxonomy, tuberculosis, Actinomycetales,
Mycobacteriaceae, Mycobacterium leprae, Mycobacterium tuberculosis.
Sales,
M.P.U.; Taylor, G.M.; Hughes, S.; Yates, M.; Hewinson, G.; Young, D.B.; Shaw,
R.J. Genetic diversity among Mycobacterium bovis isolates: a preliminary
study of strains from animal and human sources. Journal
of Clinical Microbiology. 2001; 39 (12): 4558-4562.
URL: http://jcm.asm.org/cgi/content/full/39/12/4558
NAL Call Number: QR46.J6
Descriptors: Mycobacterium bovis, genetic studies, bacterial
strains, mycobacterial diseases, human, animals, livestock, wild animals.
Sechi,
L.A.; Zanetti, S.; Sanguinetti, M.; Molicotti, P.; Romano, L.; Leori, G.;
Delogu, G.; Boccia, S.; la Sorda, M.; Fadda, G. Molecular basis of rifampin
and isoniazid resistance in Mycobacterium bovis strains isolated in
Sardinia, Italy. Antimicrobial Agents and Chemotherapy. 2001. 45 (6)
1645-1648.
NAL Call Number: RM265 A5132
Descriptors: cattle, antimycobacterial agents, drug resistance,
genetic analysis, isoniazid, leucine, mutations, nucleotide sequences, praline,
rifampicin, strains, Mycobacterium bovis,
Sielaff, B.;
Andreesen, J.R.; Schraeder, T. A cytochrome P450 and a ferredoxin
isolated from Mycobacterium sp.
strain HE5 after growth on morpholine. Applied Microbiology and Biotechnology. 2001, 56 (3/4) 458-464. ISSN: 0175-7598.
NAL Call Number:
QR1.E9
Descriptors: Mycobacterium strain HE5, Mycobacterium smegmatis,
amino acid sequence, culture media, ferredoxin,
iron-sulfur protein, molecular weight, cytochrome
450, morpholine, carbon and nitrogen sources, carbon
monoxide, piperidine, pyrrolidine.
Skuce,
R.A.; Neill, S.D.; Ellner, J.J. (ed.); Brennan,
P.J. (ed.); Young, D. Molecular epidemiology of Mycobacterium bovis:
exploiting molecular data. Tuberculosis.
2001. 81 (1-2) 169-175. Note: Third international conference on Mycobacterium
bovis, M. bovis 2000, Cambridge,
Descriptors: antigenic variation, disease transmission, epidemiology,
molecular epidemiology, molecular genetics, mycobacterial diseases, pathogenesis,
Mycobacterium bovis strains, virulence, cattle, wild animals, wildlife,
zoonotic diseases, New Zealand, reviews.
Smith,
R.A.; Alvarez, A.J.; Estes, D.M. The P2X7 purinergic receptor on
bovine macrophages mediates mycobacterial death. Veterinary
Immunology and Immunopathology. 2001; 78 (3/4): 249-262. ISSN:
0165-2427
URL: http://www.elsevier.com/wps/find/journalabstracting.cws_home/503319/abstracting
NAL Call Number: SF757.2.V38
Abstract: P2X7 is an ATP gated purinoceptor that has been linked to
various immune responses. P2X7 appears to be expressed ubiquitously in the
immune system and thus may be important as an effector pathway or play significant
roles in cell activation/death. 2',3'-(4-Benzoyl)benzoyl
ATP is the most potent agonist of this receptor and ATP in its fully dissociated
form (ATP(4-)) also activates the receptor. High concentrations of ATP can
cause the P2X7 receptor to induce pore formation on the surface of the cell
that allows molecules of considerable size to pass and can lead to cell death.
The P2X7 receptor has also been linked to various immune activities when
the concentration of ATP is lower, including the release of IL-1beta. The
role P2X7 receptors have on immune cell activities is just beginning to be
understood. We sought to determine the role of P2X7 on bovine macrophages
in eliminating the causative agent of bovine-type tuberculosis, Mycobacterium
bovis. Because high concentrations of ATP are linked to macrophage death,
we determined if this method of cell destruction also leads to reduced bacterial
viability. We find that P2X7 is present on bovine macrophages from different
sources, including both peripheral blood-derived as well as alveolar macrophages.
In addition, P2X7 mRNA is present in B and T lymphocytes. The treatment
of M. bovis-infected macrophages with ATP results in reduced macrophage
viability as well as reduced M. bovis viability.
Descriptors: cattle, macrophages, Mycobacterium bovis BCB-strain,
receptors, messenger RNA, viability, death, cell growth, ATP, B Lymphocytes,
T lymphocytes.
Smyth,
A.J.; Welsh, M.D.; Girvin, R.M.; Pollock, J.M. In vitro responsiveness
of gamma delta T cells from Mycobacterium bovis-infected cattle to
mycobacterial antigens: predominant involvement of WC1+ cells. Infection
and Immunity. 2001; 69 (1): 89-96. ISSN: 0019-9567
URL: http://iai.asm.org/
NAL Call Number: QR1.I57
Abstract: It is generally accepted that protective immunity against
tuberculosis is generated through the cell-mediated immune (CMI) system, and
a greater understanding of such responses is required if better vaccines and
diagnostic tests are to be developed. gammadelta
T cells from a major proportion of the peripheral blood mononuclear cells
(PBMC) in the ruminant system and, considering data from other species, may
have a significant role in CMI responses in bovine tuberculosis. This study
compared the in vitro responses of alphabeta and gammadelta T cells from Mycobacterium
bovis-infected and uninfected cattle. The results showed that, following
24 h of culture of PBMC with M. bovis-derived antigens, the majority
of gammadelta T cells from infected animals became highly activated (upregulation
of interleukin-2R), while a lower proportion of the alphabetaT-cell population
showed activation. Similar responses were evident to a lesser degree in uninfected
animals. Study of the kinetics of this response showed that gammadelta T
cells remained significantly activated for at least 7 days in culture, while
activation of alphabeta T cells declined during that period. Subsequent analysis
revealed that the majority of activated gammadelta T cells expressed WC1,
a 215-kDa surface molecule which is not expressed on human or murine gammadelta
T cells. Furthermore, in comparison with what was found for CD4+ T cells,
M. bovis antigen was found to induce strong cellular proliferation
but relatively little gamma interferon release by purified WC1+ gammadelta
T cells. Overall, while the role of these cells in protective immunity remains
unclear, their highly activated status in. response to M. bovis suggests
an important role in antimycobacterial immunity, and the ability of gammadelta
T cells to influence other immune cell functions remains to be elucidated,
particularly in relation to CMI-based diagnostic tests.
Descriptors: T lymphocytes, cell mediated immunity, bacterial antigens.
Sun,
Z.; Cheng, S.J.; Zhang, H.; Zhang, Y. Salicylate uniquely induces a 27-kDa
protein in tubercle bacillus. FEMS Microbiology Letters.
Sept 25,
2001.
203 (2) 211-216. ISSN: 0378-1097
NAL Call Number: QR1.F44
Abstract: Salicylate was found to uniquely induce a 27-kDa
protein in Mycobacterium tuberculosis complex organisms but not in
Mycobacterium smegmatis or Escherichia coli. The structural
analogue antitubercular para-amino-salicylate also induced the 27-kDa protein
but to a somewhat lower level than salicylate. Other structural analogues
such as benzoic acid and acetyl salicylic acid (aspirin) did not induce the
27-kDa protein. Western blot analysis indicated that the 27-kDa protein was
localized mainly in the cytoplasm. The 27-kDa protein was not expressed at
different growth phases in the absence of salicylate. The 27-kDa protein was
identified as a putative benzoquinone methyltransferase (Rv0560c), which has
several homologues in the M. tuberculosis genome. The cloned 27-kDa
gene was found to express constitutively in E. coli, M. smegmatis
and BCG with or without salicylate.
Descriptors: salicylates, Mycobacterium tuberculosis, Mycobacterium
bovis, Mycobacterium smegmatis, E. coli, phytochemicals.
Thorel, M.F.; Huchzermeyer, H.F.; Michel, A.L. Mycobacterium
avium and Mycobacterium intracellulare infection in mammals.
Revue Scientifique et Technique Office International
des Epizooties.
2001. 20 (1) 204-218. Note: In English with French and Spanish summaries.
NAL Call Number: SF781 R4
Descriptors: domestic animals, immunosuppression, post slaughter
survey, soil microbes, tuberculosis, water, cats, dogs, small mammals, wild
animals, wild birds, zoonotic diseases.
Willumsen, P.A.;
Nielsen, J.K.; Karlson, U. Degradation
of phenanthrene-analogue azaarenes
by Mycobacterium gilvum
strain LB307T under aerobic conditions. Applied Microbiology and Biotechnology.
2001, 56 (3/4) 539-544. ISSN:
0175-7598.
NAL Call Number:
QR1.E9
Descriptors: Mycobacterium gilvum,
bacterial
biodegradation of azaarenes, 5,6-benzoquinoline,
7,8-benzoquinoline, phenanthridine, aromatic hydrocarbons,
sources of carbon, nitrogen and energy, substrates concentration levels.
Zanini,
M.S.; Moreira, E.C.; Lopes, M.T.P.; Oliveira, R.S.; Leao, S.C.; Fioravanti,
R.L.; Roxo, E.; Zumarraga, M.; Romano, M.I.; Cataldi, A.; Salas, C.E. Mycobacterium
bovis: polymerase chain reaction identification in bovine lymphonode biopsies
and genotyping in isolates from Southeast Brazil by spolygotyping and restriction
fragment length polymorphism. Memorias do Instituto Oswaldo Cruz.
2001. 96 (6) 809-813.
NAL Call Number: 448.9 IN74
Descriptors: diagnostic techniques, genotypes, lymph nodes,
polymerase chain reaction, polymorphism, cattle, Mycobacterium bovis.
2000
Bigi, F.; Alito, A.; Romano, M.I.; Zumarraga, M.;
Caimi, K.; Cataldi, A. The gene encoding P27 lipoprotein and a putative
antibiotic-resistance gene form an operon in Mycobacterium tuberculosis
and Mycobacterium bovis. Microbiology. 2000. 146 (4) 1011-1018.
NAL Call Number: QR1 J64
Descriptors: lipoproteins, Mycobacterium bovis, Mycobacterium
bovis BCG strain, Mycobacterium tuberculosis,
Mycobacterium smegmatis, genes, operons, promoters, antibiotics, drug
resistance.
Braibant, M.;
Gilot, P.; Content, J. The ATP binding cassette
(ABC) transport systems of Mycobacterium
tuberculosis. FEMS Microbiology Reviews. 2000, 24 (4) 449-467. ISSN:
NAL Call Number:
QR1.F46
Descriptors: Mycobacterium tuberculosis, inventory and assembly
of subunits of ABC transporter genes, transporter genes occupy 2.5% of genome,
genome analysis, antibiotic resistance, amino acid sequences, control resistance,
proteins, bacterial attachment control, bacterial ability to synthesize essential
compounds, few external essential compounds.
Coetsier,
C.; Vannuffel, P.; Blondeel, N.; Denef, J.F.; Cocito, C.; Gala, J.L. Duplex PCR for differential identification of Mycobacterium bovis,
M. avium, and M.
avium ssp. paratuberculosis
in formalin-fixed paraffin-embedded tissues from cattle.
Journal of Clinical Microbiology. 2000. 38
(8) 3048-3054.
NAL Call Number: QR46.J6
Descriptors: cattle, deletions, differential diagnosis, DNA
sequencing, genes, identification, nucleotide sequences, open reading frames,
paratuberculosis, PCR, polymerase chain reaction, tuberculosis, Mycobacterium
avium ssp. paratuberculosis, Mycobacterium bovis, Mycobacterium tuberculosis.
Domingues-Junior, M.; Pinheiro, S.R.; Guerra, J.L.;
Palermo-Neto, J. Effects
of treatment with amphetamine and diazepam on Mycobacterium bovis-induced
infection in hamsters. Immunopharmacology and
Immunotoxicology. 2000. 22 (3) 555-574.
NAL Call Number: RM370 I55
Descriptors: hamster disease model, tuberculosis, Mycobacterium
bovis, amphetamine (AMPH) and diazepam, impaired immune defense, effects
of drugs on macrophage/lymphocyte activity.
Durr, P.A.; Hewinson, R.G.; Clifton-Hadley, R.S.
Molecular epidemiology of bovine tuberculosis. I. Mycobacterium bovis
genotyping. Revue Scientifique et Technique, Office International des Epizooties. 2000.
19 (3) 675-688. Note: In English with Spanish and French summaries.
NAL Call Number: SF781 R4
Descriptors: molecular epidemiology, Mycobacterium bovis,
tuberculosis, epidemiology, restriction endonuclease analysis, restriction
fragment length polymorphism, PCR, polymerase chain reaction, nucleotide sequences,
cattle.
Durr, P.A.; Clifton-Hadley, R.S.; Hewinson, R.G.
Molecular epidemiology of bovine tuberculosis. II. Applications of genotyping.
Revue Scientifique et Technique, Office International
des Epizooties. 2000. 19 (3) 689-701. Note: In English with Spanish and
French summaries.
NAL Call Number: SF781 R4
Descriptors: cattle, molecular epidemiology, genotypes, Mycobacterium
bovis, tuberculosis, restriction endonuclease analysis, restriction fragment
length polymorphism, nucleotide sequences.
Hatfull, Graham F.; Jacobs, William R. Molecular
genetics of mycobacteria. Washington, D.C.: ASM
Press, c2000. xii, 363 p. ISBN: 1555811914
NAL Call Number: QR82.M8 M64 2000
Descriptors: Mycobacterium, bacterial genetics, tuberculosis,
genetic aspects.
McGraw, L. Zoonoses. Agricultural
Research.
Feb 2000. 48 (2) 18-20. ISSN: 0002-161X.
URL: http://www.ars.usda.gov/is/AR/
NAL Call Number: 1.98 Ag84
Descriptors: leptospirosis, brucellosis, tuberculosis, Mycobacterium,
disease control.
Ramakrishnan, L.;
Federspiel, N.A.; Falkow, S. Granuloma-specific expression of Mycobacterium virulence proteins from the
glycine-rich PE-PGRS family. Science. 2000, 288 (5470) 1436-1439. ISSN: 0036-8075.
NAL Call Number:
470 SCI2
Descriptors: Mycobacterium marinum,
Mycobacterium tuberculosis, macrophage replication, virulence gene factor expression,
PE-PGRS gene, host granulomas, glycine-rich proteins, 2 deficient mutants, incapable of replication
in macrophages, decreased persistence in lesions.
Rishendra,Verma;
Verma, R.; Verma, R. (ed.); Sharma, N. (ed.); Varma, T.K. (ed.); Bagherwal,
R.K. (ed.); Jaiswal, T.N. TB: global emergency. Can man be infected
with bovine TB? Advancements in Veterinary Science.
Indian Association for the Advancement of Veterinary Research,
Bareilly, 2000.
p. 55-62. Note:
Descriptors: humans, cattle, zoonotic potential, Mycobacterium
tuberculosis, Mycobacterium bovis, diagnosis, treatment, disease
control, disease transmission.
Roring,
S.; Hughes, M.S.; Skuce, R.A.; Neill, S.D. Simultaneous detection and strain
differentiation of Mycobacterium bovis directly from bovine tissue
specimens by spoligotyping. Veterinary Microbiology. 2000. 74 (3) 227-236.
NAL Call Number: SF601 V44
Descriptors: Mycobacterium bovis, bovine tuberculosis,
rapid detection and strain typing, lesioned bovine lymph node specimens, PCR,
spoligotyping, decontaminated and non-decontaminated lesioned lymph nodes,
DNA, cattle.
Scanlon, M.P.; Quinn, P.J. Inactivation of Mycobacterium
bovis in cattle slurry by five volatile chemicals. Journal
of Applied Microbiology. 2000. 89 (5) 854-861.
NAL Call Number: QR1 J687
Descriptors: cattle slurry, Mycobacterium bovis, in vitro
study, acetone, ammonium hydroxide, chloroform, ethyl alcohol, xylene, farm
level use potential.
Sechi,
L.A.; Dupre, I.; Leori, G.; Fadda, G.; Zanetti, S. Distribution of a specific
500-base-pair fragment in Mycobacterium bovis isolates from Sardinian
cattle. Journal of Clinical Microbiology.
2000. 38 (10) 3837-3839.
NAL Call Number: QR46.J6
Descriptors: genetics, amplification of bacterial DNA, Mycobacterium
bovis, Mycobacterium tuberculosis, diagnosis, cattle,
Torkko, P.; Suomalainen, S.;
Iivanainen, E.; Suutari,
M.; Tortoli, E.; Paulin,
L.; Katila, M.L.
NAL Call Number:
442.8 IN82
Descriptors: Mycobacterium,
scotochromogenic organisms, stream water isolates,
GLC-MS, biochemical test, internal transcribed spacer sequencing, lipid analysis,
unique sequences, characteristics of new species, strains (E347(T) and E43),
ATCC strains700701(T) and 700702.
Vilcheze, C.; Morbidoni, H.R.; Weisbrod, T.R.;
Iwamoto, H.; Kuo, M.; Sacchettini. J.C.; Jacobs, W.R. Jr. Inactivation of the
inhA-encoded fatty acid synthase II (FASII) enoyl-acyl carrier protein reductase
induces accumulation of the FASI end products and cell lysis of Mycobacterium
smegmatis. Journal of Bacteriology. 2000. 182 (14) 4059-4067.
NAL Call Number: 448.3 J82
Descriptors: lysis, Mycobacterium bovis, Mycobacterium
smegmatis, Mycobacterium tuberculosis, fatty acid synthase, binding
proteins, genes, isoniazid.
1999
Amadori,
M.; Archetti, I.L.; Scaccaglia, P.; Modena, D.; Fossati, G.; Lucietto, P.;
Mascagni, P. Chaperonin 10 of Mycobacterium
tuberculosis induces a protective immune response to foot-and-mouth disease
virus. Archives
of Virology. 1999. 144 (5) 905-919. ISSN: 0304-8608
NAL Call Number: 448.3 Ar23
Descriptors: Mycobacterium tuberculosis,
FMD, aphthovirus, antibody formation, antiviral
properties, heat shock proteins, stress response.
Anonomyous. Tuberculosis.
Journal of Small Animal Practice. March 1999. 40 (3)
145-147. ISSN: 0022-4510. Note: This article was prepared by the British
Small Animal Veterinary Association's Scientific Committee.
NAL Call Number: 41.8 J8292
Descriptors: dogs, cats, man, Mycobacterium tuberculosis,
pathogenesis, clinical aspects, diagnosis, zoonoses.
Aranaz,
A.; Liebana, E.; Gomez-Mampaso, E.; Galan, J.C.; Cousins, D.; Ortega, A.;
Blazquez, J.; Baquero, F.; Mateos, A.; Suarez, G. Mycobacterium tuberculosis
ssp. caprae ssp. nov.: a taxonomic
study of a new member of the Mycobacterium tuberculosis complex isolated
from goats in Spain. International Journal of Systematic
Bacteriology. July 1999. 49 (pt. 3) 1263-1273. ISSN: 0020-7713
NAL Call Number: 448.3 In8
Descriptors: new subspecies, taxonomy description, Mycobacterium
tuberculosis ssp. caprae ssp. nov.,
Bulling, E.; Schonberg, A. Robert von Ostertag
(1864-1940).
A veterinarian contemporary with R.
Virchow and R. Koch. Historia Medicinae Veterinariae.
1999. 24 (4) 97-120. Note: In English with a German summary.
NAL Call Number: SF615 A1V4
Descriptors: veterinary history, biographical information, slaughter
houses, abattoirs, meat hygiene, infectious diseases, meat inspection, meat
products, pathology, slaughter, bovine tuberculosis and other zoonotic diseases,
veterinary contributions.
Costello,
E.; O'Grady, D.; Flynn, O.; O'Brien, R.; Rogers, M.; Quigley, F.; Egan, J.;
Griffin, J. Study of restriction fragment length polymorphism analysis
and spoligotyping for epidemiological investigation of Mycobacterium bovis
infection. Journal of Clinical Microbiology.
1999. 37 (10) 3217-3222.
NAL Call Number: QR46.J6
Descriptors: RFLP analysis, strain typing, Mycobacterium
bovis, IS 6110, direct repeat sequence, polymorphic GC-rich sequence,
spoligotyping, DNA fingerprinting, 452 isolates, cattle, badger, deer, pigs,
sheep, goat, indicators of infection transmission between species, Irish Republic.
de Lisle, G.W.; Wilson, T.; Collins, D.M.; Buddle,
B.M. Vaccination of guinea pigs with nutritionally impaired avirulent mutants
of Mycobacterium bovis protects against tuberculosis. Infection
and Immunity. May 1999. 67 (5) 2624-2626. ISSN: 0019-9567
NAL
Call Number: QR1.I57
Abstract: Four nutritionally impaired
strains of Mycobacterium bovis produced by illegitimate recombination
were tested for their ability to protect guinea pigs against intratracheal
challenge with virulent M. bovis. All four strains and M. bovis
BCG induced significant levels of protection as measured by the reduced
spread of infection to the spleen and liver. In animals vaccinated with BCG
or two of the other strains, the bacterial counts from the lungs were significantly
lower than those of the nonvaccinated animals.
Descriptors: introtracheal challenge, virulent strain challenge,
4 strains, levels of protection, bacterial count, lungs.
Erler, W.; Schimmel, D.; Feist, H.; Geschwend,
G. Zur Differenzierung von Mykobakterien. [Studies on the
differentiation of mycobacteria.]
Tierarztliche Umschau. 1999. 54 (10) 579-582. Note:
In German with an English summary.
NAL Call Number: 41.8 T445
Descriptors: National Veterinary Reference
Laboratory for Tuberculosis, differentiation, microbial, biochemical and molecular
biological methods, research studies completed, Mycobacterium,
Flesselles,
B.; Anand, N.N.; Remani, J.; Loosmore, S.M.; Klein, M.H. Disruption of
the mycobacterial cell entry gene of Mycobacterium bovis BCG results
in a mutant that exhibits a reduced invasiveness for epithelial cells.
FEMS Microbiology Letters. Aug 15, 1999. 177 (2) 237-242. ISSN:
0378-1097
NAL Call Number: QR1.F44
Abstract: Mycobacteria belonging to the Mycobacterium
tuberculosis complex have the ability to invade and replicate in nonphagocytic
cells, an event that requires the presence of bacterial surface components
capable of triggering a cell response and the subsequent internalization of
the microorganism. In this study, we report the sequencing of the mycobacterial
cell entry gene (mce) of Mycobacterium bovis bacillus Calmette-Guerin
(BCG) and the generation and characterization of a mutant BCG strain with
an inactivated mce gene, by homologous recombination with double cross-over.
This mutant strain does not express the mycobacterial cell entry protein (Mce)
and exhibits a reduced ability to invade the non-phagocytic epithelial cell
line HeLa as compared to wild-type BCG.
Descriptors: Mycobacterium bovis BCG strain, cell invasion.
Molecular sequence data: genbank/af113402.
Gormley,
E.; Fray, L.; Sandall, L.; Ke, GenPing; Dupont, C.; Carpenter, E. Detection
of Mycobacterium bovis lymphocyte stimulating antigens in culture filtrates
of a recombinant Mycobacterium smegmatis cosmid library. Vaccine.
1999. 17 (22) 2792-2801.
NAL Call Number: QR189 V32
Descriptors: Mycobacterium bovis, cultural filtrates, cosmid
library, Mycobacterium smegmatis, lymphocyte stimulatory antigens,
mononuclear cells from vaccinated cattle, Mycobacterium bovis BCG,
IFN-gamma production, cellular response, antigen detection assay, heterogeneity.
Kouba, V. Historie eliminace bovinni tuberkulozy
v Ceske Republice. [History of the eradication of bovine tuberculosis
in the Czech Republic.]
Casopis Lekaru Ceskych.
1999. 138 (15) 456-459. Note: In Czech with an English summary.
Descriptors: Mycobacterium bovis, eradication, history,
disease control, cattle, humans, slaughter, Czech Republic.
Montgomery, R.H.
Mycobacteria in
NAL Call Number: SF604.63 N45S87
Descriptors: birds, possums, dogs,
cats, rabbits, pigs, sheep, goats, deer, cattle, humans, Mycobacterium
taxonomy, diagnosis, disease transmission, disease prevalence, disease control,
Mycobacterium bovis, Mycobacterium tuberculosis, Erinaceidae,
Mustela erminea, Mycobacterium avium, Mycobacterium paratuberculosis,
Mycobacterium lepraemurium, Mycobacterium marinum, Mycobacterium
kansasii, New Zealand.
Nelson,
A.M. The cost of disease eradication: smallpox and bovine tuberculosis.
Annals of the New York Academy of Sciences. 1999.
894: 83-91. “Food and
agricultural security guarding against natural threats and terrorist attacks
affecting health, national food supplies, and agricultural economics.”
Note: Paper presented at the "International conference on food and agricultural
security," September 28-30, 1998, Washington, D.C.
ISBN: 1573312304.
NAL Call Number: 500 N484 v. 894
Descriptors: disease control and eradication, smallpox, cattle
diseases, tuberculosis, health care costs, disease prevention, zoonotic disease
threat, economic impacts, Mycobacterium bovis.
Pavlas,
M. The 30th anniversary of eradication of bovine tuberculosis in cattle
in Czechoslovakia. Acta Veterinaria Brno.
1999. 68 (2) 155-162. Note: In English with a Czech summary.
NAL Call Number: SF604 B7
Descriptors: Mycobacterium tuberculosis, cattle, humans, reviews,
disease prevalence, control programs, disease control, culling of diseased
animals, epidemiology, public health concerns.
Pereira, J.J.; Garbaccio, S.G. Tuberculosis bovina.
El veterinario y una enfermedad tan vieja como el mundo. [Bovine tuberculosis.
The veterinarian and a disease as old as the world.] Revista de Medicina Veterinaria,
Buenos Aires. 1999. 80 (4) 328-329. Note: In Spanish.
NAL Call Number: 41.8 B86
Descriptors: cattle, tuberculosis, Mycobacterium, epidemiology,
veterinarians.
Rauzier,
J.; Gormley, E.; Gutierrez, M.C.; Kassa-Kelembho, E.; Sandall, L.J.; Dupont,
C.; Gicquel, B.; Murray, A. A novel polymorphic genetic
locus in members of the Mycobacterium tuberculosis complex.
Microbiology. 1999. 145 (7) 1695-1701.
NAL Call Number: QR1.J64
Descriptors: Mycobacterium tuberculosis, cattle, Mycobacterium
bovis, Mycobacterium avium ssp. paratuberculosis, Mycobacterium
bovis BCG, Mycobacterium africanum, Mycobacterium microtum,
Mycobacterium paratuberculosis, polymorphism, tuberculosis, DNA probes,
genetic markers, promoters, restriction fragment length polymorphism, RFLP,
strain differences, transposable elements, gene loci.
Reynolds,
D. TB in cattle: the government's commitment to controlling the disease.
Cattle Practice. 1999. 7 (4) 371.
NAL Call Number: SF961 C37
Descriptors: tuberculosis, legislation, zoonoses, slaughter,
diagnosis, disease transmission, disease control, cattle, Mycobacterium
tuberculosis, wild badgers, Meles meles,
Reynolds,
R.C.; Bansal, N.; Rose, J.; Friedrich, J.; Suling, W.J.; Maddry, J.A. Ethambutol-sugar
hybrids as potential inhibitors of mycobacterial cell-wall biosynthesis.
Carbohydrate Research. Apr 30, 1999. 317
(1/4) 164-179. ISSN: 0008-6215
NAL Call Number: 385 C172
Abstract: Ethambutol is an established front-line agent for
the treatment of tuberculosis, and is also active against Mycobacterium
avium infection. However, this agent exhibits toxicity, and is considered
to have low potency. The action of ethambutol on the mycobacterial cell wall,
particularly the arabinan, and comparison of the structure of ethambutol with
several of the cell-wall saccharides, suggested that ethambutol-saccharide
hybrids might lead to agents with a more selective mechanism of action. To
this end, eight ethambutol-saccharide hybrids were synthesized and screened
against M. tuberculosis and several clinical isolates of M. avium.
Descriptors: Mycobacterium tuberculosis, M. avium, effectiveness,
toxicity, glycosyltransferases.
Rodriguez,
J.G.; Fissanoti, J.C.; del Portillo, P.; Patarroyo, M.E.; Romano, M.I.; Cataldi,
A. Amplification of a 500-base-pair fragment from cultured isolates of
Mycobacterium bovis. Journal of Clinical Microbiology.
1999. 37 (7) 2330-2332.
NAL Call Number: QR46 J6
Descriptors: amplification, 500 bp DNA fragment, Mycobacterium
bovis, 121 isolates, potential as a diagnostic assay, polymorphism, PCR,
DNA probes, nucleotide sequences, cattle, sea lions, Argentina, Colombia,
Mexico.
Romero, R.E.; Garzon, D.L.; Mejia, G.A.; Monroy,
W.; Patarroyo, M.E.; Murillo,
L.A. Identification
of Mycobacterium bovis in bovine clinical samples by PCR species-specific
primers. Canadian Journal of Veterinary Resources. Apr
1999. 63 (2) 101-106. ISSN: 0830-9000. Note: In English with a French summary.
NAL Call Number: SF601.C24
Descriptors: dairy cows, Mycobacterium bovis, rapid methods,
polymerase chain reaction, PCR, southern blotting, blood, mucus, milk, skin
tests, disease surveys, tuberculosis, cross reaction, dot blotting, Colombia.
Romero, R.E.; Garzon, D.L.; Mejia, G.A.; Monroy,
W.; Patarroyo, M.E.; Murillo,
L.A. Identification
of Mycobacterium bovis in bovine clinical samples by PCR species-specific
primers. Canadian Journal of Veterinary
Research. 1999. 63 (2) 101-106.
NAL Call Number: SF601.C24
Descriptors: dairy cattle, Mycobacterium bovis, tuberculosis,
diagnosis, PCR, polymerase chain reaction, diagnostic techniques, 470 bp fragment,
intradermal tuberculin test, nasal mucus sampling, PCR more specific and tensitive
diagnosis.
Roxo, E. Importancia da medicina veterinaria nos programas de combate a
tuberculose. [Importance of veterinary
medicine in programs for combating tuberculosis.]
O Biologico. 1999. 61 (2) 143-144. Note: In Portuguese.
Descriptors: disease control, tuberculosis, Mycobacterium,
zoonoses.
Sakamoto,
S.M.; Heinemann, M.B.; Telles, M.A.S.; Roxo, E.; Richtzenhain, L.J.; Vasconcellos,
S.A.; Ferreira-Neto, J.S. Deteccao e identificacao de Mycobacterium
bovis pela reacao em cadeia da polimerase (PCR). [Detection and
identification of Mycobacterium bovis using polymerase chain reaction
(PCR).] Arquivos do Instituto Biologico, Sao Paulo. 1999. 66 (2) 45-58. Note:
In Portugeuse with an English summary.
NAL Call Number: 442.9 SA6
Descriptors: Mycobacterium bovis, PCR, detection, identification
techniques.
Santillan-Flores, M.A.; Sanchez-Zamorano, L.M.;
Milian-Suazo, F.; Ramirez Casillas, I.C. Viabilidad de Mycobacterium
bovis en solucion de tetraborato de sodio. [Viability
of Mycobacterium bovis in a sodium tetraborate solution.]
Tecnica Pecuaria en
NAL Call Number: 49 T222
Descriptors: cattle, Mycobacterium bovis, lymph nodes,
sodium tetraborate storage, various storage periods, culturing, bacteria viability
over time, tissue preservation, diagnostic method.
Sutmoller,
P. Risk of disease transmission by llama embryos. Revue Scientifique et Technique Office International des Epizooties. 1999. 18
(3) 719-728. Note: In English with French and Spanish summaries.
NAL Call Number: SF781 R4
Descriptors: disease transmission risks, embryos, brucellosis,
contamination, embryo transfer, FMD, risk assessment, tuberculosis, zona pellucida,
arboviruses, bacterial diseases, viral diseases, Bluetongue virus, Brucella, Mycobacterium,
vesicular stomatitis virus, llamas.
Tanner, M; Michel, A.L. Investigation of the viability of M.
bovis under different environmental conditions in the Kruger National Park. Onderstepoort
Journal of Veterinary Research. 1999. 66 (3) 185-190.
NAL Call Number: 41.8 On1
Descriptors: environment, wildlife, African buffalo, Kruger
National Park, feces, lungs, lymph nodes, wild animals, viability, survival,
habitats, seasons, bacterial diseases, Syncerus caffer, Mycobacterium
bovis, seasonal effects, South Africa.
Wiker,
H.G.; Michell, S.L.; Hewinson, R.G.; Spierings, E.; Nagai, S.; Harboe, M.
Cloning, expression and significance of MPT53 for identification of secreted
proteins of Mycobacterium tuberculosis. Microbial
Pathogenesis. 1999. 26 (4) 207-219.
NAL Call Number: QR175 M53
Descriptors: cattle, Mycobacterium tuberculosis, antigens,
excretory-secretory products, amino acid sequences, amino acids, characterization,
gene expression, immune response, antibodies, IgM, IgG1 anti-MPT53, cattle
sera.
Vordermeier,
H.M.; Cockle, P.C.; Whelan, A.; Rhodes, S.; Palmer, N.; Bakker, D.; Hewinson,
R.G. Development of diagnostic reagents to differentiate between Mycobacterium
bovis BCG vaccination and M. bovis infection in cattle.
Clinical and Diagnostic Laboratory Immunology.
1999. 6 (5) 675-682.
Descriptors: recombinant forms of antigens, BCG Pasteur (ESAT-6,
MPB64, MPB70, MPB83), testing, Mycobacterium bovis, calf mononuclear
cells, sensitized animals, M. bovis infected, BCG vaccinated, M.
avium sensitized, in vitro proliferation and gamma interferon responses,
peptide and protein cocktails formulations, T cell epitopes.
Zumarraga, M.; Bigi, F.; Alito, A.; Romano, M.I.;
Cataldi, A.
A 12.7 kb fragment of the Mycobacterium tuberculosis genome
is not present in Mycobacterium bovis. Microbiology.
Apr 1999. 145 (pt. 4) 893-897. ISSN: 1350-0872
NAL Call Number: QR1.J64
Abstract: Southern blotting, sequence analysis and PCR experiments
showed that Mycobacterium bovis and Mycobacterium bovis BCG
lack a 12(.)7 kb fragment present in the genome of Mycobacterium tuberculosis.
This region is 337 bp downstream of the RD2 region, which was previously described
as being absent from some M. bovis BCG strains. The
12(.)7 kb fragment should be useful as a target for a PCR test to differentiate
M. tuberculosis and M. bovis. An analysis
of the 12(.)7 kb region suggests that it represents a deletion in M.
bovis rather than an insertion in M. tuberculosis. The deletion
removes most of the mce-3 operon, one of four highly related operons which
may be involved in cell entry, and therefore it may contribute to differences
in virulence or host range in the two species.
Descriptors: genetics, strain differences, RD2 region, Southern
blotting, PRC, differentiate strains, Mycobacterium bovis, Mycobacterium
bovis BCG, Mycobacterium tuberculosis. Molecular sequence data: genbank/al022073.
1998
Ballarini,
G . Tubercolosi e micobatteriosi: ieri, oggi e
domani. [Tuberculosis and mycobacterial infections: yesterday, today
and tomorrow.] Obiettivi e Documenti Veterinari.
1998. 19 (7-8) 35-42. Note: In Italian.
Descriptors: cattle, pigs, humans, veterinary history, epidemiology,
tuberculosis, Mycobacterium, cattle diseases, swine diseases.
Clifton-Hadley,
R.S.; Inwald, J.; Archer, J.; Hughes, S.; Palmer, N.; Sayers, A.R.; Sweeney,
K.; Van Embden, J.D.A.; Hewinson, R.G. Recent advances in DNA fingerprinting
using spoligotyping - epidemiological applications in bovine TB. Cattle
Practice. 1998. 6 (2) 79-82.
NAL Call Number: SF961 C37
Descriptors: 2668 Mycobacterium bovis isolates, cattle,
badgers, other species, spoligotyping, epidemiology, DNA fingerprinting,
Cornejo,
B.J.; Sahagun-Ruiz, A.; Suarez-Guemes, F.; Thornton, C.G.; Ficht, T.A.; Adams,
L.G. Comparison of C18-carboxypropylbetaine and glass bead DNA extraction
methods for the detection of Mycobacterium bovis in bovine milk samples
and analysis of samples by PCR. Applied and Environmental
Microbiology. Aug 1998. 64 (8) 3099-3101. ISSN: 0099-2240
NAL
Call Number: 448.3 Ap5
Abstract:
The purpose of this prospective study was to compare two different milk
preparation methods to assay for the presence of Mycobacterium bovis
by PCR. Detection by a C18-carboxypropylbetaine (CB-18)-based sample processing
method was compared to extraction of DNA from milk with glass beads. Samples
from 17 skin test-positive cattle were analyzed. Following CB-18 processing
and glass bead extraction, the sensitivity of IS6110-based PCR was 94.1 and
58.8%, respectively (P < 0.025). Because CB-18 processing will permit the
proficient use of PCR for diagnosis and surveillance of bovine tuberculosis,
it will contribute to the more efficient detection and control of tuberculosis.
Descriptors: polymerase chain reaction, detection methods, milk,
Mycobacterium bovis, surveillance.
Cousins,
D.; Williams, S.; Liebana, E.; Aranaz, A.; Bunschoten, A.; van Embden, J.;
Ellis, T. Evaluation of four DNA typing techniques in epidemiological investigations
of bovine tuberculosis. Journal of Clinical Microbiology. Jan 1998. 36 (1)
168-178. ISSN: 0095-1137
NAL Call Number: QR46.J6
Descriptors: Mycobacterium bovis, DNA typing techniques,
comparative study, effectiveness.
Cousins, D.V.; Skuce, R.A.; Kazwala, R.R.; Van
Embden, J.D.A. Towards a standardized approach to DNA fingerprinting of
Mycobacterium bovis. International
Journal of Tuberculosis and Lung Disease. 1998. 2 (6) 471-478.
Descriptors: DNA strain typing standards, Mycobacterium bovis,
RFLP, recommendations.
Dechering, K.J.; Cuelenaere, K.; Konings, R.N.H.;
Leunissen, J.A.M. Distinct frequency-distributions of homopolymeric DNA
tracts in different genomes. Nucleic
Acids Research. Sept 1, 1998. 26 (17) 4056-4062.
ISSN: 0305-1048
NAL Call Number: QD341.A2N8
Descriptors: Arabidopsis thaliana, Saccharomyces cerevisiae,
man, Plasmodium falciparum, Escherichia coli, Caenorhabditis
elegans, Mycobacterium tuberculosis, DNA, genomes, nucleosides,
species differences, chemical composition, base composition.
Elleingand,
E.; Gerez, C.; Un, S.; Knupling, M.; Lu, G.; Salem, J.; Rubin, H.; Sauge-Merle,
S.; Laulhere, J.P.; Fontecave, M. Reactivity studies of the tyrosyl radical
in ribonucleotide reductase from Mycobacterium tuberculosis and Arabidopsis
thaliana: comparison with Escherichia coli and mouse. European
Journal of Biochemistry. Dec 1998. 258 (2) 485-490. ISSN: 0014-2956
NAL Call Number: QP501.E8
Descriptors: tuberculosis, Mycobacterium tuberculosis,
Arabidopsis thaliana, enzyme physiology, comparison study, ribonucleotide
reductase.
Espinosa
De Los Monteros, L.E.; Galan, J.C.; Gutierrez, M.; Samper, S.; Garcia-Marin,
J.F.; Martin, C.; Dominguez, L.; de Rafael, L.; Baquero, F.; Gomez-Mampaso,
E. Allele-specific PCR method based on pncA and oxyR sequences for distinguishing
Mycobacterium bovis from Mycobacterium tuberculosis: intraspecific
M. bovis pncA sequence polymorphism. [Erratum: Aug
1998. 36 (8), p. 2398.] Journal of Clinical Microbiology.
Jan 1998. 36 (1) 239-242. ISSN: 0095-1137
NAL Call Number: QR46.J6
Descriptors: pathogenic strain differentiation, Mycobacterium
bovis from Mycobacterium tuberculosis, pncA and oxyR sequences,
sequence polymorphism.
Ficht,
T.A.; Whipple, D.; Perumaalla, V.; Chacon,O.; Alford, P.; Slater, M.; Baca,
D.; Hernandez, J.; Payeur, J.; Jarnagin, J.; Suarez, F.; Turcotte, C.; Rohonczy,
E.; Adams, L.G.; Williams, E.I. Molecular epidemiologic and geographic
information system analyses of Mycobacterium bovis isolates from North
America. Proceedings of the Thirty-First Annual Conference
American Association of Bovine Practitioners, Spokane, Washington,
NAL Call Number:
SF961 A5
Descriptors: cattle, deer, Mycobacterium bovis, epidemiology,
tuberculosis, DNA fingerprinting, RFLP, restriction fragment length polymorphism,
isolates, epidemiology, geographical information, North America.
Fisanotti,
J.C.; Alito, A.; Bigi, F.; Latini, O.; Roxo, E.; Cicuta, E.; Zumarraga, M.J.;
Cataldi, A.; Romano, M.I. Molecular epidemiology of Mycobacterium bovis
isolates from South America. Veterinary Microbiology. 1998. 60 (2/4) 251-257.
NAL Call Number: SF601 V44
Descriptors: cattle, DNA fingerprinting, 178 isolates, useful
for epidemiological studies, tuberculosis, bacterial diseases, Mycobacterium
bovis, Argentina, Paraguay, Mexico, Costa Rica, Brazil.
Fischer,
N.H.; Lu, T.; Cantrell, C.L.; Castaneda-Acosta, J.; Quijano, L.; Franzblau,
S.G. Antimycobacterial evaluation of germacranolides. Phytochemistry.
Sept 1998. 49 (2) 559-564. ISSN: 0031-9422
NAL Call Number: 450 P5622
Abstract: The minimum inhibitory concentrations (MIC) against
Mycobacterium tuberculosis and M. avium of parthenolide, costunolide,
1 (10)-epoxycostunolide and other germacranolide-type sesquiterpene lactones
and derivatives were determined by use of a radiorespirometric bioassay. Structure-activity
relationship studies with natural and semisynthetic sesquiterpene lactones
suggested that the alpha-methylene-gamma-lactone moiety is an essential, but
not sufficient, structural requirement for significant in vitro activity against
M. tuberculosis and M. avium.
Descriptors: Mycobacterium avium, Mycobacterium tuberculosis,
phytosterols, sesquiterpenoid lactones, antibacterial properties, derivatives,
bioassays, structure activity relationships, chemical structure, biochemical
pathways.
Gallagher, J.; Jenkins, P.A.; Palmer, S.R. (ed.);
Soulsby, Lord (ed.); Simpson, D.I.H. Mycobacterial diseases.
Zoonoses: Biology, Clinical Practice and Public Health Control. Oxford, Oxford University
Press. 1998. p. 155-164.
NAL Call Number: RC113.5 Z673 1998
Descriptors: Mycobacterium bovis, Mycobacterium avium,
Mycobacterium avium complex, Mycobacterium tuberculosis, zoonotic
diseases, epidemiology, disease prevention and control, treatment.
Gonzalez-Salazar, D.; Valero-Elizondo, G.; Monroy-Basilio,
J.I.; Cordova-Lopez, D.
Comparacion entre tinciones especiales para bacterias acido-resistentes en
cortes histologicos. [Comparison of stains
for acid-fast bacteria for histological sections.]
Tecnica Pecuaria en
NAL Call Number: 49 T222
Descriptors: stains, staining, Mycobacterium bovis, cattle,
tuberculosis, diagnosis, acid-fast bacteria, comparison study.
Hamid, M.E.; Ridell,
M.; Minnikin, D.E.; Goodfellow, M. Serotaxonomic analysis of glycolipids
from Mycobacterium chelonae-M. fortuitum complex and bovine farcy strains. Zentralblatt fur Bakteriologie.
1998. 288 (1) 23-34.
NAL Call Number: QR1 Z443
Descriptors: cattle, humans, glycolipids, Mycobacterium
strains, characterization, taxonomy, antigens, bacterial diseases, Mycobacterium
chelonae, Mycobacterium fortuitum, Mycobacterium farcinogenes,
Mycobacterium sengalense.
Joardar,
S.N.; Ram, G.C.; Srivastava, S.K.; Joshi, P.; Bansal, M.P. Seroreactivity
of Mycobacterium bovis AN5 culture filtrate antigens. Indian
Journal of Comparative Microbiology, Immunology and Infectious Diseases.
1998. 19 (1) 35-39.
Descriptors: Mycobacterium bovis, cattle, immunological
factors, antigens, bacterial antigens, diagnostic techniques, ELISA, tuberculosis,
bacterial proteins, immunodiagnosis, diagnosis.
Majoros, T.; Cseh, K.; Guzsvany, M. A
fureszpor szerepe a szarvasmarhak mycobacteriosisaban. [Role of sawdust in mycobacteriosis in cattle.] Magyar Allatorvosok
Lapja. 1998. 120 (9) 535-538. Note: In Hungarian with an English
summary.
NAL Call Number: 41.8 V644
Descriptors: cattle, Mycobacterium gordonae, diagnosis,
tuberculosis, sawdust, tuberculin skin tests, false positive results, sawdust
litter/bedding,
Ocepek,
M.; Posedi, J. Evaluation of gen-probe amplified Mycobacterium tuberculosis
direct test and AccuProbe culture identification test in diagnostic of animal
tuberculosis. Zbornik Veterinarske
Fakultete Univerza Ljubljana. 1998. 35 (1-2) 35-41. Note: In English with a
Slovenian summary.
NAL
Call Number: SF604 L52
Descriptors: Mycobacterium tuberculosis, Mycobacterium avium,
Mycobacterium bovis, accuracy of diagnostic techniques, efficacy of
nucleic acid hybridization test identification, MTD test, domestic animal
tuberculosis.
Pavlik, I. Bovinni
tuberkuloza zvirat a lidi v Africe. [Bovine tuberculosis in animals and people
in Africa.]
Veterinarstvi. 1998. 48 (7) 312-314.
Note: In
NAL Call Number:
41.8 V6439
Descriptors: animals, humans, Mycobacterium bovis, reviews,
tuberculosis, Africa.
Roring,
S.; Hughes, M.S.; Beck, L.A.; Skuce, R.A.; Neill, S.D. Rapid diagnosis
and strain differentiation of Mycobacterium bovis in radiometric culture
by spoligotyping. Veterinary Microbiology. 1998. 61 (1/2) 71-80.
NAL Call Number: SF601 V44
Descriptors: Mycobacterium bovis, cattle, spoligotyping,
BACTEC 12B broth cultures, bovine lymph node tissue, 7 types, ST1, ST2, ST14,
ST21, ST25, diagnosis and detection, Northern Ireland.
Tameni,
S.; Amadori, M.; Scaccaglia, P.; Quondam-Giandomenico, R.; Tagliabue, S.;
Archetti, I.L.; Adone, R.; Ciuchini, F. Quality controls and in vitro diagnostic
efficiency of bovine PPD tuberculins. Biologicals. 1998. 26 (3) 225-235.
NAL Call Number: QH301 J68
Descriptors: cattle, Mycobacterium bovis, quality controls,
tuberculin, diagnosis, tuberculosis, antigens.
Tizard,
M.; Bull, T.; Millar, D.; Doran, T.; Martin, H.; Sumar, N.; Ford, J.; Hermon-Taylor,
J. A low G+C content genetic island in Mycobacterium avium subsp.
paratuberculosis and M. avium subsp. silvaticum
with homologous genes in Mycobacterium tuberculosis. Microbiology. Dec 1998. 144
(pt. 12) 3413-3423. ISSN: 1350-0872
NAL Call Number: QR1.J64
Abstract: The technique of representation difference analysis
PCR has been applied to find genes specific to Mycobacterium avium ssp.
paratuberculosis. This generated a 671 bp fragment which was used to isolate
a larger genetic element found in the enteric pathogens M. avium ssp.
paratuberculosis and M. avium ssp. silvaticum but which
was absent from the very closely related and relatively benign M. avium subsp.
avium. This element, designated GS, is greater than 6(.)5 kbp in length and
has a G+C content 9 mol% lower than other genes from this species. There is
a previously uncharacterized insertion sequence associated with one end. The
GS element encodes five ORFs in M. avium ssp. paratuberculosis
and M. avium ssp. silvaticum, all of which have counterparts
encoded in Mycobacterium tuberculosis. Database searches revealed homologues
for these ORFs in a number of bacterial species, predominantly Gram-negative
organisms, including a number of enteric pathogens. These homologous genes
encode functions related to LPS or extracellular polysaccharide biosynthesis.
This element has a number of features in common with pathogenicity islands
such as its low G+C content, an association with a putative insertion sequence
and a grouping of genes of related function with a possible link to virulence.
No direct link to pathogenicity has been shown but GS may belong to a group
of related 'genetic islands' and represents the first such element to be identified
in mycobacteria.
Descriptors: Mycobacterium avium ssp. silvaticum,
Mycobacterium avium ssp. paratuberculosis, Mycobacterium
tuberculosis. Molecular sequence data: genbank/aj223832, genbank/aj223833
NAL Call Number: SF601 S8
Descriptors: cattle, Mycobacterium bovis, DNA fingerprinting,
tuberculosis, research, vaccine development, diagnostic techniques,
NAL Call Number: 49.9 UN3R
Descriptors: livestock, pigs, cattle, bison, horses, llamas,
poultry, aquaculture species, wildlife, animal welfare, biotechnology, disease
outbreaks, feeds, food safety, international trade, parasitoses, drugs, environment,
rabies, bluetongue virus, Retroviridae, Leptospira, Aujeszky virus,
Salmonella, Mycobacterium bovis, Mycobacterium avium ssp.
paratuberculosis, USA.
Wang, Z.G. Isolation and identification of atypical
mycobacteria from cattle. Journal of Jilin Agricultural University. 1998. 20 (2) 73-77. Note:
In Chinese with an English summary.
Descriptors: atypical strain, cattle, lymph nodes, post slaughter
tissue harvesting, Mycobacterium scrofulaceum, Mycobacterium fortuitum,
Mycobacterium avium intracellulare complex, Mycobacterium paratuberculosis,
Mycobacterium flavescens, an unknown rapid grower, China.
Wren,
B.W.; Stabler, R.S.; Das, S.S.; Butcher, P.D.; Mangan, J.A.; Clarke, J.D.;
Casali, N.; Parish, T.; Stoker, N.G. Characterization of a haemolysin from
Mycobacterium tuberculosis with homology to a virulence factor of Serpulina
hyodysenteriae. Microbiology. May
1998. 144 (pt. 5) 1205-1211. ISSN: 1350-0872
NAL Call Number: QR1.J64
Abstract: Scrutiny of sequence data from the Mycobacterium
leprae genome sequencing project identified the presence of a gene encoding
a 268-amino-acid polypeptide which is highly similar to a pore-forming haemolysin/cytotoxin
virulence determinant, TlyA, from the swine pathogen Serpulina hyodysenteriae.
Using degenerate oligonucleotide primers based on the TlyA sequences, the
Mycobacterium tuberculosis homologue was amplified and this product
was used to obtain the clone and sequence a 2.5 kb fragment containing the
whole M. tuberculosis tlyA gene. tlyA encodes a 267-amino-acid protein with a predicted molecular
mass of 28 kDa. TlyA homologues were identified by PCR in M. leprae,
Mycobacterium avium and Mycobacterium bovis BCG, but appeared
absent in Mycobacterium smegmatis, Mycobacterium vaccae, Mycobacterium
kansasii, Mycobacterium chelonae and Mycobacterium phlei.
The M. tuberculosis gene appeared to be the first gene in an operon
containing at least two other genes. Introduction of the M. tuberculosis
tlyA gene into M. smegmatis using a mycobacterial shuttle expression
plasmid converted non-haemolytic cells into those exhibiting significant haemolytic
activity. Similarly, inducible haemolytic activity was observed in sonicated
bacteria when tlyA was expressed as a His6-tagged fusion protein in Escherichia
coli. tlyA mRNA was detected in both M. tuberculosis and M.
bovis BCG using RT-PCR, confirming that this gene is expressed in organisms
cultured in vitro.
Descriptors: virulence facts, tlyA homologues, PCR, Mycobacterium
leprae, Mycobacterium avium, Mycobacterium bovis BCG, Mycobacterium
smegmatis, Mycobacterium vaccae, Mycobacterium kansasii,
Mycobacterium chelonae, Mycobacterium phlei. Molecular sequence data: genbank/x98295.
Yao,
J.Z.; Xu, C.B.; Jin, F.S.; Feng, S.Z.; Zhu, P. Nucleotide sequence analysis
of the hsp 70 gene promoter of Mycobacterium tuberculosis.
Chinese Journal of Veterinary Science. 1998.
18 (6) 562.
NAL Call Number: SF604 C58
Descriptors: Mycobacterium tuberculosis, genes, nucleotide sequences.
Yearsley, D.; O'Rourke, J.; O'Brien, T.; Egan,
J. Comparison of three methods for the isolation of mycobacteria from bovine
tissue lesions. Veterinary
Record. 1998. 143 (17) 480-481.
ISSN: 0042-4900
NAL Call Number: 41.8 V641
Descriptors: cattle, lymph nodes, Mycobacterium tuberculosis,
isolation techniques, rapid methods, culture media, cell culture.
1997
Bigi, F.; Espitia, C.; Alito, A.; Zumarraga, M.;
Romano, M.I.; Cravero, S.; Cataldi, A. A novel 27 kDa lipoprotein
antigen from Mycobacterium bovis. Microbiology.
Nov 1997. 143 (pt. 11) 3599-3605. ISSN: 1350-0872
NAL
Call Number: QR1.J64
Abstract: A novel Mycobacterium bovis antigen was
identified from an expression library using sera from naturally infected cattle.
The Escherichia coli recombinant clone expressed a 27 kDa protein,
named P27. A rabbit serum against the recombinant antigen recognized a protein
of 27 kDa in cellular extracts from M. bovis and M. tuberculosis.
No protein was recognized in the culture supernatant. Sequence analysis indicated
that P27 has a molecular mass of 24 kDa, showing a characteristic signal sequence
for lipoprotein modification (a signal peptidase type II site). The gene is
identical to a gene identified in the M. tuberculosis genome sequencing
project. Cellular fractionation experiments suggested that P27 is an integral
membrane protein. The antigen was recognized by individual sera and peripheral
blood mononuclear cells (PBMC) from diseased cattle. PCR experiments with
specific primers directed to the P27 structural gene indicated that it is
only present in the M. tuberculosis species complex. In conclusion,
a novel immunogenic lipoprotein in M. bovis/M. tuberculosis has been
identified. The results presented here and elsewhere suggest that mycobacterial
lipoproteins should be considered in the design of new recombinant vaccines
and diagnostic methods.
Descriptors: cattle, nucleotide sequences, amino acid sequences,
Mycobacterium bovis, Mycobacterium tuberculosis. Molecular sequence data: genbank/aj000500.
Ficht,
T.A.; Whipple, D.; Perumaalla, V.; Chacon, O.; Alford, P.; Slater, M.; Baca,
D.; Hernandez, J.; Payeur, J.; Jarnagin, J.; Suarez, F.; Turcotte, C.; Rohonczy,
E.; Adams, L.G. Molecular epidemiologic and geographic information system
analyses of Mycobacterium bovis isolates from North America. Proceedings
One Hundred and First Annual Meeting of the United States Animal Health Association,
Louisville, Kentucky, USA, 18-24 October, 1997. Richmond,
NAL Call Number: 49.9 UN3R
Descriptors: cattle, deer, Mycobacterium bovis, strain
isolates, epidemiology, restriction fragment length polymorphism, RFLP, tuberculosis,
DNA fingerprinting,
Sun, L.; Kang, D.; Ge, X.; Sun, J.H.; Li, R.Z.
Application of PPA-ELISA for the detection of avian tuberculosis antibody
in chicken serum. Chinese
Journal of Animal Quarantine. 1997. 14 (3) 11-13. Note: In Chinese.
Descriptors: Mycobacterium avium, antibodies, rabbit anti-chicken
IgG immune serum, diagnostic test, PPA-ELISA, chickens, experimental infections,
test sensitivity, test specificity and cost, SPF flocks.
Wilson,
T.; Wards, B.J.; White, S.J.; Skou, B.; de Lisle, G.W.; Collins, D.M. Production
of avirulent Mycobacterium bovis strains by illegitimate recombination
with deoxyribonucleic acid fragments containing an interrupted ahpC gene.
Tubercle and Lung Disease. 1997. 78 (5/6) 229-235.
Descriptors: DNA, recombination, illegitatimate recombinants,
genes, vaccines, tuberculosis, Mycobacterium bovis ATCC35723, electroporation,
kanamycin resistance, inability for growth in minimal medium, linear fragment
approach, avirulent auxotrophs.