D.J.; Manvell, R.J.; Lowings, J.P.; Frost, K.M.; Collins, M.S.; Russell, P.H.;
Smith, J.E. Antigenic diversity and
similarities detected in avian paramyxovirus type 1 (Newcastle disease virus) isolates using monoclonal antibodies. Avian Pathology. June 1997. v. 26 (2) p. 399-418. ISSN: 0307-9457 Note: Summaries in French, German and Spanish.
call no: SF995.A1A9
Abstract: Newcastle disease (ND) virus (APMV-1)
isolates submitted to the International Reference Laboratory for ND were characterised
antigenically by their ability to cause binding of mouse monoclonal antibodies
(mAbs) to cell cultures infected with the isolate. Since the availability of
the mAbs 1526 viruses have been examined using a panel of nine mAbs and 818
with an extended panel of 26 mAbs. Using the nine mAb panel a total of 14 different
patterns was seen and viruses grouped by the same pattern showed relationships
with each other which were either biological, temporal or geographical or more
than one of these. There was a marked tendency of viruses placed in the same
group to show similar virulence for chickens. Extension of the panel to 26 mAbs
produced 39 distinct patterns, although some of these were seen with only a
single virus. Again, viruses inducing similar binding patterns shared similar
properties and some binding patterns were specific for viruses causing discrete
epizootics. Cluster analysis of the mAb binding patterns did not produce concise,
discrete groupings, but did emphasise some relationships between virus properties
and antigenicity. Examples of the usefulness of this approach were the ability
to link two important outbreaks to the contamination of stored food by infected
feral pigeons, and the demonstration of two separate viruses responsible for
outbreaks in countries of the European Union during 1991 to 1994 thus preventing
erroneous epizootiological tracing.
Descriptors: Newcastle disease virus, antigenic
variation, monoclonal antibodies, virulence, chickens, antigens, binding, cluster
H.F.; Kelly, P.J.; De Angelis, N.D.; Rohde, C.; Collins, N.;
Zulu, T. Comparison of enzyme-linked immunosorbent assay and haemagglutination
inhibition test for the detection of antibodies against Newcastle disease virus in ostriches (Struthio camelus). Avian Pathology. June
1997. v. 26 (2) p. 357-363. ISSN: 0307-9457
Note: Summaries in French, German and
call no: SF995.A1A9
Abstract: Reactivity of ostrich sera to Newcastle disease virus (NDV) by
enzyme-linked immunosorbent assay (ELISA) and haemagglutination inhibition (HI)
test were compared. Ten-month old ostriches seronegative by both tests were
vaccinated with an oil-based NDV vaccine on days 0 and 11. Significant levels
of reactive antibodies were first detected on day 11 by ELISA (sample/positive
ratio > 0.2 in 11/20 birds; 55%) and HI (titre > 1/8 in 10/20 birds; 50%).
At the end of the experiment (day 37) all birds had significant antibody levels
by ELISA, but only 16/20 (80%) by HI test. There was a sigmoidal relationship
(r= 0.62, 3rd degree polynomial) between antibody levels detected by ELISA and
by HI test. Antibodies reactive with NDV in naturally exposed ostriches from
Zimbabwe and Botswana were also detected by
ELISA (112/165; 68%) and HI (85/165; 52%).
Descriptors: ostriches, Newcastle disease virus, ELISA,
hemagglutination inhibition test, antibodies, detection, vaccination, Newcastle disease, diagnostic value.
R.; Mirza, A.M.; Mahon, P.J.; Iorio, R.M. Functional
chimeric HN glycoproteins derived from Newcastle disease virus and human parainfluenza virus-3. Archives
of Virology, Supplementum. 1997. (suppl. 13) p. 115-130. ISSN: 0939-1983 Note: Paper presented at the 9th Munich Symposium
on Microbiology entitled "Viral Zoonoses and Food of Animal Origin: a Re-Evaluation
of Possible Hazards for Human Health," Munich. Edited by O.R. Kaaden,
C.P. Czerny, and W. Eichhorn.
call no: QR355.A72
Descriptors: Newcastle disease virus, parainfluenza 3 virus,
chimeras, envelope proteins, viral hemagglutinins, sialidase, enzyme activity,
molecular conformation, amino acid sequences.
W.; Emmerson, P.T. Assembly of recombinant Newcastle disease virus nucleocapsid protein into nucleocapsid-like
structures is inhibited by the phosphoprotein. The Journal
of General Virology. Sept 1997. v. 78 (pt. 9) p. 2335-2339. ISSN: 0022-1317
call no: QR360.A1J6
Abstract: A recombinant baculovirus expressing the nucleocapsid
gene (NP) of Newcastle disease virus (NDV), a
member of the genus Rubulavirus, has been generated and shown to express the
native protein to high levels in insect cells. In contrast to the NP protein
of the rubulavirus human parainfluenza virus 2, the NDV protein has been demonstrated
by electron microscopy and caesium chloride gradient analysis to be capable
of self-assembly in vivo to form nucleocapsid-like structures in the absence
of other NDV proteins. These structures, which contained RNA that was resistant
to micrococcal nuclease digestion, were also observed when the protein was expressed
in E. coli, a phenomenon which was not inhibited by the presence of a 40 amino
acid fusion region at the amino terminus of the protein. Further, the formation
of these structures was inhibited by the co-expression of the phosphoprotein
(P). Therefore, we conclude that the P protein acts as a chaperone, preventing
uncontrolled encapsulation of non-viral RNA by NP protein.
Descriptors: nucleocapsid protein gene, phosphoprotein inhibition,
Raphael Deladem. Early Diagnosis and Control of Newcastle Disease in Chickens. 1997.
vii, 86 leaves, ill. Note: Thesis (M.S.)--University of Minnesota, 1997.
chickens, diagnosis, prevention and control.
A.; Sklan, D. Effect of dietary fatty acids on humoral immune response of turkeys.
British Poultry Science. Sept 1997. v. 38 (4) p. 342-348. ISSN: 0007-1668
call no: 47.8 B77
Abstract: 1. This study examined the effect of increasing
amounts of dietary polyunsaturated fatty acids on the fatty acid composition
in serum and antibody production following a standard vaccination programme
in growing turkeys. Turkey poults were fed on 5 diets containing 75g/kg added
fat made up of different proportions of palm and soyabean oils, and were vaccinated
against Newcastle disease, infectious bronchitis and necrotic enteritis according
to a standard vaccination programme. Blood samples were taken before and one
week after each vaccination. 2. Fatty acid composition in serum reflected the
composition of the diets although arachidonic acid concentration was not changed
by dietary fatty acid content. Growth, erythrocyte and leukocyte parameters
were not affected by the respective diets. 3. Specific antibody production was
related quadratically to serum linoleic and total n-6 polyunsaturated fatty
acid concentrations. No correlation was found with linolenic or arachidonic
acids. 4. It is concluded that dietary fatty acid composition can augment the
specific anti-vaccine immune response in turkey poults.
Descriptors: turkeys, dietary fat, polyenoic fatty acids,
vaccination, palm oils, soybean oil, antibody formation, blood serum, linoleic
acid, linolenic acid, arachidonic acid.
R.A.; Best, M.; Jordan, L.T.; Dulac, G.C.; Eddington,
D.L.; Sterritt, W.G. Efficacy of vaporized hydrogen peroxide against
exotic animal viruses. Applied and Environmental Microbiology.
Oct 1997. v. 63 (10) p. 3916-3918. ISSN: 0099-2240
call no: 448.3 Ap5
Abstract: The efficacy of vapor-phase hydrogen peroxide
in a pass-through box for the decontamination of equipment and inanimate materials
potentially contaminated with exotic animal viruses was evaluated. Tests were
conducted with a variety of viral agents, which included representatives of
several virus families (orthomyxoviridae, Reoviridae, Flaviviridae, Paramyxoviridae,
Herpesviridae, Picornaviridae. Caliciviridae, and Rhabdoviridae) from both avian
and mammalian species, with particular emphasis on animal viruses exotic to
Canada. The effects of the gas
on a variety of laboratory equipment were also studied. Virus suspensions in
cell culture media, egg fluid, or blood were dried onto glass and stainless
steel. Virus viability was assessed after exposure to vapor-phase hydrogen peroxide
for 30 min. For all viruses tested and under all conditions (except one), the
decontamination process reduced the virus titer to 0 embryo-lethal doses for
the avian viruses (avian influenza and Newcastle disease viruses) or less than
10 tissue culture infective doses for the mammalian viruses (African swine fever,
bluetongue, hog cholera, pseudorabies, swine vesicular disease, vesicular exanthema,
and vesicular stomatitis viruses. The laboratory equipment exposed to the gas
appeared to suffer no adverse effects. Vapor-phase hydrogen peroxide decontamination
can be recommended as a safe and efficacious way of removing potentially virus-contaminated
objects from biocontainment level III laboratories in which exotic animal disease
virus agents are handled.
Descriptors: decontamination, biocontainment,
exotic animal viruses, virus contaminated objects, orthomyxoviridae, Reoviridae,
Flaviviridae, Paramyxoviridae, Herpesviridae, Picornaviridae. Caliciviridae,
E.D.; Levy, A.M.; Vaiman, R.; Schwartsburd, B. Chicken-embryo
fibroblasts produce two types of interferon upon stimulation with Newcastle disease virus. Veterinary Immunology and Immunopathology.
v. 57 (3/4) p. 289-303. ISSN: 0165-2427
call no: SF757.2.V38
Abstract: Controversy has long surrounded the question
of whether chickens, like mammals, can produce two types of interferon (IFN).
Recently, type-I and type-II chicken IFNs have been cloned. Our study focuses
on the further characterization of native fibroblast and spleen IFNs and shows
that chicken embryo fibroblasts (CEFs) produce a mixture of type-I and type-II
IFNs. IFN was purified by three different methods, controlled pore-glass chromatography,
ion-exchange chromatography and preparative SDS-PAGE. Three protein bands showing
IFN-like anti-viral activity, from CEFs which had been virus-stimulated for
IFN production, were detected at 25, 27 and 29 kDa. Polyclonal antibodies produced
against these bands showed partial cross-reaction with purified media from mitogen-stimulated
spleen cells in ELISA, western blot analysis and anti-viral activity neutralization
assay. Differences between purified conditioned media from CEF and spleen were
found with respect to the stimulation of macrophages for nitric oxide production,
pH stability and signal transduction pathways; only CEF IFN activated the IFN-stimulated
gene factor-3 complex, whereas both CEF and spleen IFNs activated the IFN regulatory
factor-1 gene. These findings concur with the differences that are known to
exist between mammalian type-I and type-II IFNs. Attempts at sequencing the
25 and 27 kDa proteins by Edman degradation yielded evidence of N-terminal blockage.
Descriptors: interferon development, chicken fibroblasts.
A.; Koch, G.; van Roozelaar, D.J.; Balk, F.; Huurne, A. ter. Differentiation
of virulent and non-virulent strains of Newcastle disease virus within 24 hours
by polymerase chain reaction. Avian Pathology. Dec
1997. v. 26 (4) p. 837-849. ISSN: 0307-9457
Note: Summaries in French, German, and Spanish.
call no: SF995.A1A9
Abstract: Fast diagnosis of Newcastle disease is a prerequisite
for confining outbreaks. Diagnosis implies the differentiation of virulent and
non-virulent Newcastle disease viruses (NDV).
However, conventional methods, i.e. isolation of the virus and determination
of the intracerebral pathogenicity index, take at least 5 days. Therefore, we
investigated whether diagnosis can be performed by using the reverse transcriptase-polymerase
chain reaction (RT-PCR) on RNA isolated directly from tissue homogenate. Two
oligonucleotide primers, representing the sequence at the cleavage site of the
F protein of either virulent or non-virulent NDV strains, respectively, were
used to differentiate NDV. Using the RT-PCR we were able to differentiate 15
NDV reference strains, 11 of which were virulent and 14 non-virulent. The RT-PCR
was further validated by using homogenate of brain, trachea, lung and spleen
from 12 chicken flocks and one turkey flock suspected of Newcastle disease. The RT-PCR detected
virulent NDV in samples of seven flocks and non-virulent NDV in two out of three
flocks in agreement with conventional methods. However the RT-PCR failed to
detect virus in 1/3 flocks from which non-virulent virus was isolated. The results
are discussed. We conclude that the RT-PCR described can be used to confirm
diagnosis of Newcastle disease within 24 h using
RNA isolated directly from tissue homogenate.
Descriptors: Newcastle disease virus, virulence,
strain differences, differential diagnosis, polymerase chain reaction, reverse
transcriptase, RNA, rapid methods.
D.J.; Seal, B.S. Biological and molecular
characterization of Newcastle disease virus isolates from surveillance of live bird markets
in the northeastern United States. Avian Diseases. July/Sept 1997. v. 41 (3) p. 683-689. ISSN: 0005-2086
Note: Spanish summary.
call no: 41.8 Av5
Abstract: Newcastle disease virus (NDV) is
frequently recovered from surveillance samples collected by U.S. Department
of Agriculture, Animal and Plant Health Inspection Service personnel in live
bird markets. Six NDV isolates, five from chickens and one from a pheasant,
were characterized for comparison with reference NDV
isolates from poultry and other birds. All isolates tested were of low
virulence for chickens. Four of the six isolates were similar to reference lentogens
B1 and La Sota, but two isolates, one from a chicken and one from a pheasant,
were different. The aberrant chicken isolate had a monoclonal antibody-binding
profile like an unusual Canadian pigeon isolate. Sequence analysis of the matrix
gene of this isolate demonstrated that it differed from all isolates included
in the comparison and therefore may represent a third phylogenetic NDV group.
The pheasant isolate had a monoclonal antibody-binding profile typical of other
U.S. NDV lentogens but had a matrix gene sequence and hemagglutinin thermostability
similar to strains Ulster and Queensland V4 (QV4),
viruses originally isolated in Northern Ireland and Australia, respectively. The pheasant
virus is the first lentogen isolated in the United States known to be closely related
phylogenetically to Ulster and QV4. The unusual chicken
and pheasant isolates were readily shed from the intestinal tract during chicken
passage, whereas the other isolates were shed from the respiratory tract with
little or no intestinal shedding. The frequency in live bird markets of viruses
similar to those previously thought to be exotic to the United States is unknown.
Descriptors: chickens, pheasants, Newcastle disease virus, virulence,
characterization, nucleotide sequences, genetic diversity, phylogenetics, infectivity,
thermal properties, Northeastern States, USA.
Molecular sequence data: genbank/u79551. genbank/u79552. genbank/u79553.
M.; Hutchings, D.; Cave, N.A. Cell-mediated and humoral immune responses
in broiler chickens maintained on diets containing different levels of vitamin
A. Poultry Science. Oct 1997. v. 76 (10) p. 1368-1378. ISSN: 0032-5791
call no: 47.8 Am33P
Abstract: Broiler chickens were examined for the effects
of low (400 IU/kg), standard (1,500 IU/kg), or high (15,000 IU/kg) dietary vitamin
A (VitA) levels on immune responsiveness postimmunization to Newcastle disease virus (NDV). A
control pair-fed group (1,500 IU/kg) was included to compensate for the reduced
feed intake associated with diet containing the low level of VitA. Interdigital
skin reactions to phytohemagglutinin (PHA) and CD4:CD8 T lymphocyte ratios were
significantly reduced in chickens fed the low VitA diet, whereas their antibody
responses to NDV were significantly increased as compared to birds that consumed
the 1,500 to 15,000 VitA diet ad libitum. On the other hand, birds on the high
VitA diet had reduced lymphocyte responses to concanavalin A and pokeweed, but
not to PHA. No effect of dietary VitA was observed for natural killer activity,
nor on levels of percentage of cells expressing Class II MHC antigens among
groups that consumed feed ad libitum. The results indicated that both humoral
and cellular immune responses were modulated by levels of VitA in the diet,
and suggest that VitA-deficient chickens developed a T helper (Th)2 immune response,
whereas the chickens fed highly enriched VitA diet showed a Th1 immune response.
Descriptors: broilers, retinol, dosage, humoral immunity,
cell mediated immunity, lymphocyte transformation, natural killer cells, cytotoxic
T lymphocytes, T4 lymphocytes, T8 lymphocytes, spleen, weight, liver, body weight,
skin, allergic reactions, antibody formation.
P.; Anandan, S.; Ravikumar, G.; Koteeswaran, A.; Venugopalan, A.T. Use of
egg yolk in seromonitoring against Newcastle disease. Tropical Animal Health and Production.
Nov 1997. v. 29
(4) p. 245-246. ISSN: 0049-4747
call no: SF601.T7
Descriptors: hens, serological surveys, Newcastle disease virus, egg yolk,
seroconversion, hemagglutination tests, solvents, chloroform.
P.; Venugopalan, A.T. Agar-gel-immunodiffusion
and counterimmunoelectrophoresis for diagnosis of Newcastle disease. Tropical Animal Health and Production.
Nov 1997. v. 29
(4) p. 231-234. ISSN: 0049-4747 Note: Summaries
in French and Spanish.
call no: SF601.T7
Descriptors: chickens, experimental infections, Newcastle disease virus, immunodiffusion
tests, tissues, hemagglutination tests, rapid methods, counterimmunoelectrophoresis,
P.H.; Dwivedi, P.N.; Davison, T.F. The effects of cyclosporin A and cyclophosphamide
on the populations of B and T cells and virus in the Harderian gland of chickens
vaccinated with the Hitchner B1 strain of Newcastle disease virus. Veterinary
Immunology and Immunopathology. Dec
v. 60 (1/2) p. 171-185. ISSN: 0165-2427
call no: SF757.2.V38
Descriptors: chickens, Newcastle disease
virus, vaccination, strains, ciclosporin, cyclophosphamide, B lymphocytes, T
lymphocytes, glands animal, immune response, immunohistochemistry, antigens,
cytoplasm IGM, viral replication, concanavalin A.
H.; Mitchell, B.; Brugh, M. In ovo vaccination of chicken embryos with experimental
Newcastle disease and avian influenze oil-emulsion vaccines. Avian Diseases. Oct/Dec 1997. v. 41 (4) p. 856-863. ISSN: 0005-2086
call no: 41.8 Av5
Abstract: Inactivated oil-emulsion (OE) Newcastle disease (ND) and avian
influenza (AI) vaccines were injected into 18-day-old white rock (WR) and white
leghorn (WL) chicken embryos to evaluate their immunologic efficacy and their
effects on hatchability. Embryonating eggs were inoculated at 1.5 inches depth
with various vaccine volumes and antigen concentrations. Serum hemagglutination-inhibition
(HI) titers were first detected in chickens at 2 wk posthatch. Protection against
morbidity and mortality was demonstrated in all of 10 chickens vaccinated as
embryos and challenged with viscerotropic velogenic ND virus at 53 days of age
and also in all of eight in ovo- vaccinated chickens challenged with highly
pathogenic AI virus at 34 days of age. All of five unvaccinated control chickens
for each respective ND- and AI-vaccinated group died. In pooled groups from
successive hatches, the hatchability of WR or WL embryos injected with 100 microliters
of vaccine was not significantly different (P > 0.05) from unvaccinated hatchmate
controls when needle gauges of 22, 20, and 18 were used. Seroconversion rates
of chickens vaccinated as embryos ranged from 27% to 100% with ND vaccination
and 85% to 100% for AI vaccination. For ND, geometric mean HI titers of chickens
per vaccine group ranged from 11 to 733, and in pooled groups, the range was
49 to 531. Titers for AI vaccine groups ranged from 156 to 1178. This study
demonstrated that acceptable hatchability, seroconversion rates, and protective
immunity can be attained with in ovo inoculation of ND or AI OE vaccines if
the vaccines are prepared with sufficient antigen and administered properly.
Descriptors: chick embryos, vaccination,
Newcastle disease virus, avian influenzavirus, viral diseases, vaccines efficacy,
evaluation, egg hatchability, dosage, antigens, morbidity, mortality, pathogenicity,
application equipment, seroconversion, immunity, formulations, needle gauges.
H.D. Newcastle disease oil emulsion vaccines
prepared with animal, vegetable, and synthetic oils. Avian Diseases. July/Sept 1997. v. 41 (3) p. 591-597. ISSN: 0005-2086
Note: Spanish summary.
call no: 41.8 Av5
Abstract: Animal, vegetable, and
synthetic oils were tested as potential replacements for mineral oil in Newcastle disease oil emulsion vaccines.
Emulsifying surfactants of seed oil origin comprised 10% of the oil phase that
was used to prepare water-in-oil emulsion vaccines that contained a final concentration
of 20% aqueous antigen. The hemagglutination inhibition responses of chickens
inoculated with 46 of the newly formulated oil vaccines were, in most cases,
not significantly different from chose of control chickens inoculated with mineral
oil vaccine. Tissue reactions associated with animal, vegetable, and synthetic
oil vaccines were less severe than chose associated with mineral oil vaccines.
Viscosity of the mineral oil formulations ranged from 1/2 to 3 1/2 times that
of the mineral oil control vaccines. These findings indicate that any of several
oils may be more suitable than mineral oil for preparation of immune adjuvants
for poultry vaccines.
Descriptors: Newcastle disease virus, vaccines,
vaccine development, animal and plant oils, emulsions, surfactants, chickens,
adverse effects, viscosity.
J.; Morrison, T.G. Detection of an interaction between the HN and F proteins in Newcastle disease virus-infected cells. Journal
of Virology. Sept 1997. v. 71 (9) p. 6287-6295. ISSN: 0022-538X
call no: QR360.J6
Abstract: For many paramyxoviruses, including Newcastle disease virus (NDV), syncytium
formation requires the expression of both surface glycoproteins (HN and F) in
the same cell, and evidence suggests that fusion involves a specific interaction
between the HN and F proteins. Because a potential interaction in paramyxovirus-infected
cells has never been demonstrated, such as interaction was explored by using
coimmunoprecipitation and cross-linking. Both HN and F proteins could be precipitated
with heterologous antisera after a 5-min radioactive pulse as well as after
a 2-h chase in nonradioactive medium, but at low levels. Chemical cross-linking
increased detection of complexes containing HN and F proteins at the cell surface.
After cross-linking, intermediate- as well as high-molecular-weight species
containing both proteins were precipitated with monospecific antisera. Precipitation
of proteins with anti-HN after cross-linking resulted in the detection of complexes
which electrophoresed in the stacker region of the gel, from 160 to 300 kDa,
at 150 kDa, and at 74 kDa. Precipitates obtained with anti-F after cross-linking
contained species which migrated in the stacker region of the gel, between 160
and 300 kDa, at 120 kDa, and at 66 kDa. The three to four discrete complexes
ranging in size from 160 to 300 kDa contained both HN and F proteins when precipitated
with either HN or F antisera. That cross-linking of complexes containing both
HN and F proteins was not simply a function of overexpression of viral glycoproteins
at the cell surface was addressed by demonstrating cross-linking at early time
points postinfection, when levels of viral surface glycoproteins are low. Use
of cells infected with an avirulent strain of NDV showed that chemically cross-linked
HN and F proteins were precipitated independent of cleavage of F0. Furthermore,
under conditions that maximized HN protein binding to its receptor, there was
no change in the percentages of HN and F0 proteins precipitated with heterologous
antisera, but a decrease in F1 protein precipitated was observed upon attachment.
These data argue that the HN and F proteins interact in the rough endoplasmic
reticulum. Upon attachment of the HN protein to its receptor, the HN protein
undergoes a conformational change which causes a conformational change in the
associated F protein, releasing the hydrophobic fusion peptide into the target
membrane and initiating fusion.
Descriptors: viral hemagglutinins,
sialidase, viral proteins, fusion protein.
G.; Jayakumar, R.; Kumanan, K.; Venugopalan, A.T.; Nachimuthu, K. Latex
immunoassay for rapid detection of Newcastle disease virus. Tropical Animal Health and Production.
Nov 1997. v. 29
(4) p. 227-230. ISSN: 0049-4747
call no: SF601.T7
Descriptors: Newcastle disease virus, latex agglutination
test, accuracy, rapid methods, tissues, hemagglutination inhibition test.
Y.; Shimoyamada, M.; Watanabe, K. Bindings of ovomucin to Newcastle disease virus and anti-ovomucin antibodies and its heat stability
based on binding abilities. Journal of Agricultural and Food Chemistry.
Dec 1997. v. 45 (12) p. 4629-4634.: ISSN:
call no: 381 J8223
Abstract: The bindings of ovomucin, its chemically modified
compounds, including its disulfide-reduced and alkylated alpha- and beta-subunits,
and desialylated ovomucin to NDV and anti-ovomucin antibodies were determined
by ELISA. We found that the NeuAc residue in the beta-subunit greatly contributed
to the binding of ovomucin to NDV, and disulfide bonds in ovomucin contributed
to the binding of ovomucin to antibodies. The conformational, biological, and
chemical alterations of ovomucin heated at 60-100 degrees C for 10 min under
the various pH conditions (pH 6-12) were examined on the changes in the ability
to NDV and anti-ovomucin antibodies which were also determined by ELISA, along
with determinations of SDS-PAGE patterns and CD spectra. Ovomucin degraded together
with the increases in temperature and pH, depending on destruction of NeuAc
in beta-subunit, and cleavages of disulfide bonds in inter- and intrasubunits
and peptide bonds in alpha- and beta-subunits.
Descriptors: ovomycin binding, Newcastle
Disease virus, anti-ovomycin, ELISA, SDS-PAGE patterns, CD spectra, temperatue,
Y.; Shimoyamada, M.; Watanabe, K. Structural features of Newcastle Disease Virus-and anti-ovomucin antibody-binding glycopeptides
from pronase-treated ovomucin. Journal of Agricultural
and Food Chemistry.
July 1997. v. 45 (7) p. 2393-2398. ISSN: 0021-8561
call no: 381 J8223
Abstract: Pronase-treated ovomucin was applied on a Sephacryl
S-400 column chromatography and separated into five fractions. The SDS-polyacrylamide
gel electrophoretic pattern, and amino acid and carbohydrate compositions, of
each of the obtained fractions were compared to those of ovomucin and its alpha-
and beta-subunits. Subsequently, bindings of each fraction to hen newcastle disease virus (NDV) and
anti-ovomucin antibodies were estimated. It was found that the P1, P2, and P3
fractions from the beta-subunit which were composed of O-glycoproteins, containing
more or less cluttered sialic acid moieties, had higher binding activity to
NDV, while the peptide-rich P4 and P5 fractions mainly derived from the alpha-subunit
had higher binding activity to the anti-ovomucin antibodies.
Descriptors: Newcastle disease virus, egg proteins,
proteolysis, protein subunits.
R.; Boshoff, C.H.; Verwoerd, D.; Schoeman, M.; Van Wyk, A.; Gerdes, T.H.; Roos,
K. Detection of antibodies
to Newcastle disease virus in ostriches (Struthio camelus) by an indirect
Diseases. Oct/Dec 1997. v. 41 (4) p. 864-869. ISSN: 0005-2086 Note: Spanish
call no: 41.8 Av5
Abstract: A two-graph receiver operating characteristic
analysis, performed on the hemagglutination-inhibition (HI) and enzyme-linked
immunosorbent assay (ELISA) test results of a Newcastle disease virus (NDV)-positive
and NDV-negative control group of ostrich sera, proved that the ELISA was superior
to the HI in both sensitivity and specificity. Comparison of results of the
two assays performed on a panel of simulated positive sera ranging from very
weak to very strong showed that the ELISA was at least 10 times more sensitive
than the HI in detecting low levels of ostrich antibodies to NDV. The ELISA
also has the advantage of using untreated serum in a single dilution as opposed
to the HI test, which uses pretreated serum in titration.
Descriptors: ostriches, antibodies, Newcastle disease virus, antibody
testing, ELISA, hemagglutination inhibition test, diagnosis, evaluation, comparisons,