Azarova, IA; Mishaeva, NP; Votiakov, VI; Golovneva, GP. Poisk ingibitorov virusa Zapadnogo Nila sredi antibiotikov. [Search for inhibitors of West Nile virus among antibiotics]. Antibiotiki i Khimioterapiia. 1992 Aug; 37(8): 29-31. ISSN: 0235-2990. In Russian.

Descriptors: comparison study, antibiotics, experimental laboratory infection, albino mouse model, gentamicin, kanamycin, efficacy of chemotherapeutic agents, disease prevention and treatment.   

Abstract: The activity of 24 antibiotics was studied in treatment of albino mice with experimental encephalitis caused by West Nile virus. The antiviral activity of gentamicin and kanamycin was stated. The survival rate of the animals 19. contaminated with 10-100 LD50 of the West Nile virus and treated parenterally with gentamicin in a dose of 80 to 400 micrograms/mouse was higher than that in the controls by 29.5 to 100 per cent and depended on the drug regimen. The efficacy of kanamycin was lower. The chemotherapeutic indices of gentamicin and kanamycin amounted to 100 and 10, respectively. Since there are no schemes for chemotherapy of the infection caused by the West Nile virus and the respective vaccines are not available the use of the antibiotics and gentamicin in particular appears to be promising in the disease prevention and treatment.

Ben-Nathan, D; Lustig, S; Kobiler, D; Danenberg, HD; Lupu, E; Feuerstein, G. Dehydroepiandrosterone protects mice inoculated with West Nile virus and exposed to cold stress. Journal of Medical Virology. 1992 Nov; 38(3): 159-66. ISSN: 0146-6615.

Descriptors: DHEA, anti-stressor, cold stress, experimental infection with WNV, mice, mortality levels, WNV, WN—25, modulation of host response.   

Abstract: The protective effect of pretreatment with dehydroepiandrosterone (DHEA) on stress-enhanced viral encephalitis was studied in mice exposed to cold following inoculation with West Nile virus (WNV). Exposure of WNV-inoculated mice to cold water (1 0.5 degrees C, 5 minutes/day for 8 days) resulted in a mortality rate of 83% as compared to 50% in nonstressed mice (p < 0.05). The effect of cold stress was more pronounced when mice were inoculated with WN-25, a noninvasive neurovirulent variant of WNV. Mice infected with WN-25 showed no mortality, whereas cold stressed mice inoculated with the same virus had a mortality rate of 67% (p < 0.05). The administration of DHEA (serial injections of 10-20 mg/kg with or without a loading dose of 1 gm/kg) resulted in a significant reduction in the mortality rate of stressed mice inoculated with either virus (p < 0.05). Virus levels in the blood and brain of the DHEA-treated mice, were significantly lower than in the control groups. DHEA also prevented the involution of lymphoid organs in stressed mice. The present study provides direct evidence of the protective effects of DHEA as an "anti-stress" agent. Its ability to prevent mortality associated with WNV or WN-25, and involution of lymphoid organs caused by stress-induced immunosuppression, supports the notion that its activity is based on the modulation of the host response.


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February 26, 2003