Food Safety Research Information Office
Title:Comparative Genomic Systems for Molecular Detection and Control of Toxigenic Fusarium
Objective:

The overall goal of this project is to contribute to the effective control of toxigenic Fusarium, especially those responsible for FHB of small grain cereals, in order to enhance food safety and crop production in the U.S. and around the world. The proposed project is designed to produce a robust evolutionary framework for understanding the genetic and phenotypic diversity, geographic distribution and population biology of toxigenic fusaria, and will result in novel technologies for the rapid detection, identification and control of toxigenic fusarial pathogens of critical importance to food safety and food security. This framework will also support and facilitate work by the global Fusarium research community. The specific objectives are:

Objective 1: characterize the genetic diversity and mycotoxin potential of Fusarium head blight and other fusarial pathogens, develop novel pathogen detection technologies, and expand web-accessible informational database to facilitate the rapid and accurate identification of toxigenic fusaria via the Internet.

Objective 2: Determine the global population structure of F. graminearum and identify genetic variation associated with population-level difference in growth, reproduction, and toxin accumulation phenotypes as a means to improve pathogen modeling and surveillance.

Objective 3: Characterize the mechanisms that drive the diversification and adaptive evolution of virulence genes in Fusarium genomes as well as host defense genes involved in immunogenicity and disease response.

More Info:

Toxins produced by plant-pathogenic Fusarium pose a significant threat to food safety and place a major burden on the world’s agricultural economy. The primary objectives of the proposed research are to:

1) Characterize the genetic diversity and mycotoxin potential of Fusarium head blight (FHB) and other fusarial pathogens, develop novel pathogen detection technologies, and expand web-accessible informational databases to facilitate the rapid and accurate identification of toxigenic fusaria via the Internet,

2) Determine the global population structure of F. graminearum and identify genetic variation associated with population-level differences in growth, reproduction, and toxin accumulation phenotypes as a means to improve pathogen modeling and surveillance, and

3) Characterize the mechanisms that drive the diversification and adaptive evolution of virulence genes in Fusarium genomes as well as host defense genes involved in immunogenicity and disease response. These complementary objectives are directed at developing robust pathogen control strategies through elucidating the evolution, population structure, phenotypic diversity, host range, geographic distribution and adaptive potential of toxigenic fusaria. In addition, the planned research will produce enhanced methods for identification and characterization (e.g. toxin type, toxin accumulation potential, and host preference) of Fusarium responsible for mycotoxin contamination of cereals and other food. The information and molecular tools developed as a result of this project will address the needs of small grain cereal producers, food and feed processors, the U.S. Department of Agriculture (USDA) Federal Grain Inspection Service (FGIS), the USDA Animal and Plant Health Inspection Service (APHIS), and the Food and Drug Administration (FDA).

Funding Source:United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
Type:Appropriated
Start Date:2011
End Date:2016
Project Number:3620-42000-046-00
Accession Number:421049
Institutions:USDA/ARS - Midwest Area
Investigators:O'Donnell, Kerry
Rooney, Alejandro (Alex)
Ward, Todd
Project Reports:2013 Annual Report
2012 Annual Report
2011 Annual Report
Pending USDA ARS Publications (NP 108):
Published Journal
Articles USDA
ARS (NP 108):
Population Structure of Listeria monocytogenes Serotype 4b Isolates from Sporadic Human Listeriosis in the United States, 2003-2008
Lee S, Ward TJ, Graves LM, Tarr CL, Siletzky RM, Kathariou S.
Appl Environ Microbiol. 2014 Jun;80(12):3632-44.
Diversity of the Fusarium graminearum species complex on French cereals
Boutigny A, Ward TJ, Ballois N, Iancu G, Ioos R .
Eur J Plant Pathol. 2014 Jan;138(1):133-48.
Regional differences in species composition and toxigenic potential among Fusarium head blight isolates from Uruguay indicate a risk of nivalenol contamination in new wheat production areas
Umpierrez-Failache M, Garmendia G, Pereyra S, Rodriguez A, Ward TJ, Vero S.
Int J Food Microbiol. 2013 Aug 16;166(1):135–40.
An inordinate fondness for Fusarium: phylogenetic diversity of fusaria cultivated by ambrosia beetles in the genus Euwallacea on avocado and other plant hosts
Kasson MT, O'Donnell K, Rooney AP, Sink S, Ploetz RC, Ploetz JN, Konkol JL, Carrillo D, Freeman S, Mendel Z, Smith JA, Black AW, Hulcr J, Bateman C, Stefkova K, Campbell PR, Geering AD, Dann EK, Eskalen A, Mohotti K, Short DP, Aoki T, Fenstermacher KA, Davis DD, Geiser DM.
Fungal Genet Biol. 2013 Jul;56:147-57.
Genetic determinants for cadmium and arsenic resistance among listeria monocytogenes serotype 4b from sporadic human listeriosis
Lee S, Rakic-Martinez M, Graves LM, Ward TJ, Siletzky RM, Kathariou S .
Appl Environ Microbiol. 2013 Apr;79(7):2471-6.
Phylogenetic relationships among members of the Fusarium solani species complex in human infections and the descriptions of F. keratoplasticum sp. nov. and F. petroliphilum stat. nov
Short DP, O'Donnell K, Thrane U, Nielsen KF, Zhang N, Juba JH, Geiser DM.
Fungal Genet Biol. 2013 Apr;53:59-70.
Genetic determinants for cadmium and arsenic resistance among Listeria monocytogenes serotype 4b isolates from sporadic human listeriosis patients
Lee S, Rakic-Martinez M, Graves LM, Ward TJ, Siletzky RM, Kathariou S.
Appl Environ Microbiol. 2013 Apr;79(7):2471-6.
Phylogenetic analyses of RPB1 and RPB2 support a middle Cretaceous origin for a clade comprising all agriculturally and medically important fusaria
O'Donnell K, Rooney AP, Proctor RH, Brown DW, McCormick SP, Ward TJ, Frandsen RJ, Lysøe E, Rehner SA, Aoki T, Robert VA, Crous PW, Groenewald JZ, Kang S, Geiser DM.
Fungal Genet Biol. 2013 Mar;52:20-31.
Systematics, Phylogeny and Trichothecene Mycotoxin Potential of Fusarium Head Blight Cereal Pathogens
Aoki T, Ward TJ, Kistler H, O'Donnell K.
Mycotoxins. 2012 Oct 21;62(2):91-102.
Heavy metal and disinfectant resistance of Listeria monocytogenes from foods and food processing plants
Ratani SS, Siletzky RM, Dutta V, Yildirim S, Osborne JA, Lin W, Hitchins AD, Ward TJ, Kathariou S.
Appl Environ Microbiol. 2012 Oct;78(19):6938-45.
Two novel type II restriction-modification systems occupying genomically equivalent locations on the chromosomes of Listeria monocytogenes strains
Lee S, Ward TJ, Siletzky RM, Kathariou S.
Appl Environ Microbiol. 2012 Apr;78(8):2623-30.
Atypical Listeria monocytogenes serotype 4b strains harboring a lineage II-specific gene cassette
Lee S, Ward TJ, Graves LM, Wolf LA, Sperry K, Siletzky RM, Kathariou S.
Appl Environ Microbiol. 2012 Feb;78(3):660-7.
Widespread occurrence of diverse human pathogenic types of the fungus Fusarium detected in plumbing drains
Short DP, O'Donnell K, Zhang N, Juba JH, Geiser DM.
J Clin Microbiol. 2011 Dec;49(12):4264-72.
Novel Fusarium head blight pathogens from Nepal and Louisiana revealed by multilocus genealogical concordance
Sarver BA, Ward TJ, Gale LR, Broz K, Kistler HC, Aoki T, Nicholson P, Carter J, O'Donnell K.
Fungal Genet Biol. 2011 Dec;48(12):1096-107.
Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST
O'Donnell K, Humber RA, Geiser DM, Kang S, Park B, Robert VA, Crous PW, Johnston PR, Aoki T, Rooney AP, Rehner SA.
Mycologia. 2011 Nov 15;104(2):427-45.
Analysis of the Fusarium graminearum species complex from wheat, barley and maize in South Africa provides evidence of species-specific differences in host preference
Boutigny AL, Ward TJ, Van Coller GJ, Flett B, Lamprecht SC, O'Donnell K, Viljoen A.
Fungal Genet Biol. 2011 Sep;48(9):914-20.
Non-Journal Publications:
North American isolates of Fusarium graminearum produce a novel type A trichothecene - (Abstract Only)
(18-Jun-14)
Systematics of key phytopathogenic fusaria: Current status and future challenges - (Review Article)
Aoki, T., O'Donnell, K., Geiser, D.M. 2014. Systematics of key phytopathogenic Fusarium species: current status and future challenges. Journal of General Plant Pathology. 80(3):189-201.
Composition of the Fusarium graminearum species complex populations in wheat cropping environments in Southern Brazil - (Abstract Only)
(25-Oct-13)
Survey and competition assay data suggest species-specific difference in host/niche adaptation influence the distribution of Fusarium graminearum species complex pathogens in Brazil - (Abstract Only)
Spolti, P., Kuhnem, P.R., Ward, T.J., Del Ponte, E.M. 2013. Survey and competition assay data suggest species-specific difference in host/niche adaptation influence the distribution of Fusarium graminearum species complex pathogens in Brazil. Meeting Abstract.
Species diversity, pathogenicity and toxigenicity of Fusarium associated with rice seeds in Brazil - (Abstract Only)
(25-Oct-13)
Species and trichothecene genotypes of the Fusarium graminearum species complex causing head blight of wheat in Rio Grande do Sul State, 2011 season - (Abstract Only)
(25-Oct-13)
Fusarium spp. associated with head blight of wheat in South Africa - (Abstract Only)
(25-Oct-13)
Analysis of the Fusarium graminearum species complex in Brazil shows high diversity and changes in species prevalence affected by host and geographic region - (Abstract Only)
Del Ponte, E.M., Ward, T.J., Tessman, D.J., Kuhnem, P.R., Silva, C.N., Gomes, L.B., Spolti, P., Nicoli, C.P. 2013. Analysis of the Fusarium graminearum species complex in Brazil shows high diversity and changes in species prevalence affected by host and geographic region. Meeting Abstract.
Head blight of wheat in South Africa is associated with numerous Fusarium species and chemotypes - (Abstract Only)
Van Coller, G.J., Boutigny, A., Ward, T.J., Lamprecht, S.C., Viljoen, A. 2013. Head blight of wheat in South Africa is associated with numerous Fusarium species and chemotypes. Meeting Abstract.
Metabolomic studies for the interaction Glycine max- Fusarium tucumaniae - (Abstract Only)
Scandiani, M.M., Luque, A., O Donnell, K., Aoki, T., Spampinato, C., Cervigni, G. 2013. Metabolomic studies for the interaction Glycine max- Fusarium tucumaniae. Meeting Abstract.
Fusarium Pathogenomics - (Review Article)
Ma, L., Geiser, D.M., Proctor, R.H., Rooney, A.P., O'Donnell, K., Trail, F., Gardiner, D.M., Manners, J.M., Kazan, K. 2013. Fusarium Pathogenomics. Annual Review of Microbiology. 67:399-416.
One Fungus, One Name: Defining the genus Fusarium in a scientifically robust way that preserves longstanding use - (Other)
Geiser, D.M., Aoki, T., Bacon, C.W., Baker, S.E., Bhattacharyya, M.K., Brandt, M.E., Brown, D.W., Burgess, L.W., Coleman, J.J., Correll, J.C., Covert, S.F., Crous, P.W., Cuomo, C.A., De Hoog, G., Elmer, W.H., Epstein, L., Frandsen, R.J., Freeman, S., Glenn, A.E., Gordon, T.R., Hanson, L.E., Del Mar Jimenez-Gasc, M., Kang, S., Kistler, J., Kuldau, G.A., Mccormick, S.P., O Donnell, K., Proctor, R., Rehner, S.A., Robert, V.A., Rooney, A.P., Thrane, U., Van Diepeningen, A., Ward, T.J., Wingfield, M.J., Yang, X, et. al. 2013. One Fungus, One Name: Defining the genus Fusarium in a scientifically robust way that preserves longstanding use. Phytopathology. 103(5):400-408.
Diversity of the Fusarium complex on French maize - (Abstract Only)
Ballois, N., Boutigny, A., Ward, T.J., Gourdain, E., Ioos, R. 2013. Diversity of the Fusarium complex on French maize. Meeting Abstract.
Phylogenetically marking the limits of the genus Fusarium for post-Article 59 usage - (Abstract Only)
Geiser, D.M., Rooney, A.P., Aoki, T., Kang, S., Rehner, S.A., O Donnell, K. 2012. Phylogenetically marking the limits of the genus Fusarium for post-Article 59 usage [abstract]. Mycological Society of America.
The Fusarium graminearum species complex comprises at least 16 phylogenetically distinct head blight pathogens - (Abstract Only)
Aoki, T., Ward, T.J., Kistler, H.C., O Donnell, K. 2012. The Fusarium graminearum species complex comprises at least 16 phylogenetically distinct head blight pathogens. Meeting Abstract.
Molecular characterization of true morels (Morchella) in Turkey - (Abstract Only)
Taskin, H., Buyukalaca, S., Dogan, H.H., Rehner, S.A., O Donnell, K. 2011. Molecular characterization of true morels (Morchella) in Turkey. Meeting Abstract.
Systematics, Phylogeny and Trichothecene Mycotoxin potential of Fusarium head blight cereal pathogens - (Abstract Only)
Aoki, T., Ward, T.J., Gale, L.R., O Donnell, K. 2011. Systematics, Phylogeny and Trichothecene Mycotoxin potential of Fusarium head blight cereal pathogens. Meeting Abstract.
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