Food Safety Research Information Office
Title:Biology and Control of Human Pathogens on Fresh Produce

Objective 1: Identify and chacterize the microbial genes that are involved in the attachment, colonization and survival of enteric pathogens on produce.

Objective 2: Determine the genetic and biochemical factors in plants that effect the attachment, growth and survival of human pathogens in/on plants.

Objective 3: Assess the role of other microflora and aerosols in survival and transmission of enteric pathogens in agricultural environments.

Objective 4: Develop methods for the detection of enteric viral and bacterial pathogens from produce and soil.

More Info:
Approach: Plant-microbe model systems in combination population studies, molecular methods, genomics, and microscopy, will be used to investigate the interaction of human pathogens with plants and plant-associated bacteria, as well as well as to develop improved methods for detection of human pathogens on produce. Replacing 5325-42000-044-00D 03/2011.
Funding Source:United States Department of Agriculture (USDA), Agricultural Research Service (ARS)
Start Date:2010
End Date:2015
Project Number:5325-42000-046-00
Accession Number:421196
Institutions:USDA/ARS - Pacific West Area
Investigators:Brandl, Maria
Carter, John Mark
Carter, Michelle
Cooley, Michael
Gorski, Lisa
Mandrell, Robert
Ravva, Subbarao
Tian, Peng
Project Reports:2013 Annual Report
2012 Annual Report
2011 Annual Report
Published Journal
Articles USDA
ARS (NP 108):
Prevalence of Shiga toxin producing Escherichia coli, Salmonella enterica and Listeria monocytogenes at public access watershed sites in a California central coast agricultural region
Cooley MB, Quiñones B, Oryang D, Mandrell RE, Gorski L.
Front Cell Infect Microbiol. 2014 Mar 4;4:30.
Occurrence of generic E. coli, E. coli O157 and Salmonella spp. in water and sediment from leafy green produce farms and streams on the Central California coast
Benjamin L, Atwill ER, Jay-Russell M, Cooley MB, Carychao DK, Gorski LA, Mandrell RE .
Int J Food Microbiol. 2013 Jul;165(1):65-76.
Development of a robust method for isolation of shiga toxin-positive Escherichia coli (STEC) from fecal, plant, soil and water samples from a leafy greens production region in California
Cooley MB, Jay-Russell M, Atwill ER, Carychao D, Nguyen K, Quiñones B, Patel R, Walker S, Swimley M, Pierre-Jerome E, Gordus AG, Mandrell RE.
PLoS One. 2013 Jun 6;8(6):e65716.
Diversity of pulsed-field gel electrophoresis pulsotypes, serovars, and antibiotic resistance among Salmonella isolates from wild amphibians and reptiles in the California Central Coast
Gorski L, Jay-Russell MT, Liang AS, Walker S, Bengson Y, Govoni J, Mandrell RE.
Foodborne Pathog Dis. 2013 Jun;10(6):540-8.
Hericium erinaceus (Lion's Mane) mushroom extracts inhibit metastasis of cancer cells to the lung in CT-26 colon cancer-tansplanted mice
Kim SP, Nam SH, Friedman M.
J Agric Food Chem. 2013 May 22;61(20):4898-904.
Inactivation of Tulane virus, a novel surrogate for human norovirus
Tian P, Yang D, Quegley C, Chou M, Xi J.
J Food Prot. 2013 Apr;76(4):712-8.
Identification of Salmonella enterica genes with a role in persistence on lettuce leaves during cold storage by recombinase-based in vivo expression technology
Kroupitski Y, Brandl MT, Pinto R, Belausov E, Tamir-Ariel D, Burdman S, Sela Saldinger S.
Phytopathology. 2013 Apr;103(4):362-72.
Salmonella interactions with plants and their associated microbiota
Brandl MT, Cox CE, Teplitski M.
Phytopathology. 2013 Apr;103(4):316-25.
Focus on food safety: Human pathogens on plants
Brandl MT, Sundin GW.
Phytopathology. 2013 Apr;103(4):304-5.
The salmonella transcriptome in lettuce and cilantro soft rot reveals a niche overlap with the animal host intestine
Goudeau DM, Parker CT, Zhou Y, Sela S, Kroupitski Y, Brandl MT.
Appl Environ Microbiol. 2013 Jan;79(1):250-62.
Genes ycfR, sirA and yigG contribute to the surface attachment of Salmonella enterica Typhimurium and Saintpaul to fresh produce
Salazar JK, Deng K, Tortorello ML, Brandl MT, Wang H, Zhang W.
PLoS One. 2013;8(2):e57272.
Survival characteristics of diarrheagenic Escherichia coli pathotypes and Helicobacter pylori during passage through the free-living ciliate, Tetrahymena sp
Smith CD, Berk SG, Brandl MT, Riley LW.
FEMS Microbiol Ecol. 2012 Dec;82(3):574-83.
RcsB contributes to the distinct stress fitness among Escherichia coli O157:H7 curli variants of the 1993 hamburger-associated outbreak strains
Carter MQ, Parker CT, Louie JW, Huynh S, Fagerquist CK, Mandrell RE.
Appl Environ Microbiol. 2012 Nov;78(21):7706-19.
Detection of human norovirus in cherry tomatoes, blueberries and vegetable salad by using a receptor-binding capture and magnetic sequestration (RBCMS) method
Pan L, Zhang Q, Li X, Tian P.
Food Microbiol. 2012 Jun;30(2):420-6.
Bacterial communities in urban aerosols collected with wetted-wall cyclonic samplers and seasonal fluctuations of live and culturable airborne bacteria
Ravva SV, Hernlem BJ, Sarreal CZ, Mandrell RE.
J Environ Monit. 2012 Feb;14(2):473-81.
Evolutionary silence of the acid chaperone protein HdeB in enterohemorrhagic Escherichia coli O157:H7
Carter MQ, Louie JW, Fagerquist CK, Sultan O, Miller WG, Mandrell RE.
Appl Environ Microbiol. 2012 Feb;78(4):1004-14.
Application of a receptor-binding capture quantitative reverse transcription-PCR assay to concentrate human norovirus from sewage and to study the distribution and stability of the virus
Tian P, Yang D, Pan L, Mandrell R.
Appl Environ Microbiol. 2012 Jan;78(2):429-36.
Functional metagenomics of Escherichia coli O157:H7 interactions with spinach indigenous microorganisms during biofilm formation
Carter MQ, Xue K, Brandl MT, Liu F, Wu L, Louie JW, Mandrell RE, Zhou J.
PLoS One. 2012;7(9):e44186.
Selective enrichment media bias the types of Salmonella enterica strains isolated from mixed strain cultures and complex enrichment broths
Gorski L.
PLoS One. 2012;7(4):e34722.
Non-Journal Publications:
Intra and inter-strain differences in fitness of Escherichia coli O157:H7 to protozoan predation and survival in soil - (Abstract Only)
Accepted Publication (18-Mar-13)
Variation in reactive oxygen species concentration among lettuce types and modulation of their production via PPO during E. coli O157:H7 colonization of injured lettuce leaves - (Abstract Only)
Brandl, M., Hua, S.T., Zhou, Y., Sarreal, S.L. 2012. Variation in reactive oxygen species concentration among lettuce types and modulation of their production via PPO during E. coli O157:H7 colonization of injured lettuce leaves(Abstract). Meeting Abstract. [abstract].
Food Safety Categories:On-Farm Food Safety
Pathogen Biology
Plant Science & Plant Products
Farm-to-Table Categories:On-farm food production
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