Title: Differential expression of three members of the multidomain adhesion CCp family in babesia bigemina, babesia bovis and theileria equi
Authors: Bastos R, Suarez CE, Laughery J, Johnson WC, Ueti MW, Knowles Jr DP
Journal: PLoS One
Accepted date: 2013 May 23
Interpretive summary: Members of the CCp protein family have been previously described to be expressed on gametocytes of apicomplexan Plasmodium parasites. It is known that knocking out Plasmodium CCp genes blocks the development of the parasite in the mosquito vector, making the CCp proteins potential targets for the development of a transmission-blocking vaccine. Apicomplexans Babesia bovis and Babesia bigemina are the causative agents of bovine babesiosis, and apicomplexan Theileria equi causes equine piroplasmosis. Bovine babesiosis and equine piroplasmosis are the most economically important parasite diseases that affect worldwide cattle and equine industries, respectively. The recent sequencing of the B. bovis and T. equi genomes has provided the opportunity to identify novel genes involved in parasite biology. Here we characterize three members of the CCp family, named CCp1, CCp2 and CCp3, in B. bigemina, B. bovis and T. equi. Using B. bigemina as an in vitro model for sexual stages, expression of all CCp genes and proteins was demonstrated in gametocyte-induced parasites. This study demonstrates a differential pattern of parasite expression of three newly identified members of the multidomain adhesion CCp family by B. bigemina, B. bovis and T. equi. The novel CCp members represent potential targets for innovative approaches to control bovine babesiosis and equine piroplasmosis.
Publication date: 2013 Jul 3