Title: Detection by Hyperspectral Imaging of Shiga Toxin-Producing Escherichia coli Serogroups O26, O45, O103, O111, O121, and O145 on Rainbow Agar
Authors: Windham WR, Yoon SC, Ladely SR, Haley JA, Lawrence KC, Park B, Narang N, Cray Jr WC
Journal: J Food Prot
Accepted date: 2013 Mar 7
Interpretive summary: Hyperspectral imaging was used to detect and classify non-O157 Shiga-toxin producing Escherichia coli (STEC) serogroups O26, O45, O103, O111, O121, and O145 on Rainbow Agra (RBA) O157 plates. Detection of non-O157 STEC colonies for conformational testing is challenging due to the lack of distinct color variation among serogroups grown RBA. Hyperspectral imaging is an optical imaging technique that combines aspects of conventional imaging and vibrational spectroscopy so that data can provide two-dimensional spatial information on colony shapes and one-dimensional spectral information at every pixel in each colony under test. Spectral libraries of pure pathogen cultures were built, classification models developed and tested on non-O157 STEC serogroups artificially inoculated into ground beef. Results showed the potential of the hyperspectral imaging technique for rapid presumptive positive screening of non-O157 STEC in meat products.
Publication date: 2013 Jul
Related projects: Optical Detection of Food Safety and Food Defense Hazards