Table 5


Examples of Technology Development NRI Plant Genome Grants 
1991-94
 


 Antibody synthesis for proline
 Biolistic transformation with high molecular weight DNA
 Characterize cytokinin induced MRNA stability
 Characterize tjbiquitin regulation
 Chromosome painting
 Construction and insertion of chimeric gene
 Construction of directed transpositions
 Detection of microsatellite loci
 Develop system for import of nucleic acid
 Develop vectors for plant YAC transformation
 Develop target probes for fly resistance
 DNA vector synthesis
 Functional cloning of disease resistant genes
 Identification of effective sterility genes
 In situ hybridization
 Isolate nuclear skeletal structures
 Map based cloning system
 Map polymorphic simple sequence repeats
 Megabase DNA isolation
 Molecular marker assisted screening
 Molecular probe and reagent development
 Post transcriptional control
 Regeneration after transformation for Pine
 Site directed mutagenesis
 Site-selected insertion
 Statistical methods to map QTLs
 Substrate features required for enzyme activation
 Transfer teosinte chromosomes to maize
 Transformation system for Peanut
 Transposon tagging
 Wound induced transformation