| SacBll | Ad10 | |
|---|---|---|
| Cloning | BamHI* | BamHI/SalI |
| Screening Method | positive Kan/sucrose | antibiotic Kan/Tet |
| Rare 8-base R.E. sites | SfiI/NotI | none |
| RNA Analysis | Sp6/T7 | none |
| *SalI can also be used to clone inserts, but this prevents use of Sp6 and T7 promoters to prepare RNA-probes from cloned insert. | ||