Table 1. Features of SacBll and Ad10 Plasmid Vectors
SacBllAd10
CloningBamHI*BamHI/SalI
Screening Methodpositive
Kan/sucrose
antibiotic
Kan/Tet
Rare 8-base
R.E. sites
SfiI/NotInone
RNA AnalysisSp6/T7none
*SalI can also be used to clone inserts, but
this prevents use of Sp6 and T7 promoters
to prepare RNA-probes from cloned insert.