Table 1. Features of SacBII and Ad10 Plasmid Vectors

	SacBII	Ad10
Cloning	BamHI*	BamHI / Sal I
Screening Method	positive Kan/sucrose	antibiotic Kan/Tet
Rare 8-base R.E. sites	Sfi I/NotI	none
RNA Analysis	Sp6/T7	none
*Sal can also be used to clone inserts, but this prevents use of Sp6 and T7 promoters to prepare RNA-probes from cloned insert