Table 5

Examples of Technology Development NRI Plant Genome Grants 1991-94
Antibody synthesis for proline	Map based cloning system
Biolistic transformation with high molecular weight DNA	Map polymorphic simple sequence repeats
Characterize cytokinin induced MRNA stability	Megabase DNA isolation
Characterize tjbiquitin regulation	Molecular marker assisted screening
Chromosome painting	Molecular probe and reagent development
Construction and insertion of chimeric gene	Post transcriptional control
Construction of directed transpositions	Regeneration after transformation for Pine
Detection of microsatellite loci	Site directed mutagenesis
Develop system for import of nucleic acid	Site-selected insertion
Develop vectors for plant YAC transformation	Statistical methods to map QTLs
Develop target probes for fly resistance	Substrate features required for enzyme activation
DNA vector synthesis	Transfer teosinte chromosomes to maize
Functional cloning of disease resistant genes	Transformation system for Peanut
Identification of effective sterility genes	Transposon tagging
In situ hybridization	Wound induced transformation
Isolate nuclear skeletal structures