Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM)
The murine local lymph node assay (LLNA) is a stand-alone test method for assessing the hazard potential of a test substance to induce allergic contact dermatitis (ACD). ICCVAM evaluated the use of the LLNA for potency categorization of chemicals causing ACD in humans. ICCVAM concluded that the LLNA can be used to categorize substances as strong sensitizers (Globally Harmonized System of Classification and Labeling of Chemicals Subcategory 1A), although it cannot be considered a stand-alone assay to categorize substances as other sensitizers (Globally Harmonized System of Classification and Labeling of Chemicals Subcategory 1B). Strong sensitizers are those substances considered to have a significant potential for causing hypersensitivity.