Eye Safety Testing

Information on test methods that reliably identify substances with the potential to cause eye injury and that replace, reduce, and refine animal use.

The National Toxicology Program - Interagency Center for the Evaluation of Alternative Toxicological Methods

The National Toxicology Program (NTP) Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM), on behalf of the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), announces the availability of this report that describes and evaluates four ocular toxicity test methods evaluated by ICCVAM.

Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM)

An ICCVAM recommendation to U.S. Federal agencies allows the identification of chemical eye hazards with fewer animals. ICCVAM concluded that using a classification criterion of one or more positive animals in a three-animal test maintains hazard classification equivalent to that provided by current procedures cited in 16 CFR 1500.42 while using 50% to 83% fewer animals. ICCVAM recommended consideration of this classification criterion with ocular toxicity testing procedures that use a maximum of three animals per test substance. Consistent with ICCVAM’s duty to foster interagency and international harmonization (42 U.S.C. 285l-3), this recommendation harmonized the number of animals used for ocular toxicity testing across U.S. regulatory agencies and international test guidelines.

NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM) and the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM).

The recommendations include protocols that describe how to use topical anesthetics and systemic analgesics prior to and after test article administration in order to avoid animal pain and distress. They also identify specific clinical signs and lesions that can be used as humane endpoints to allow the investigator to end a study early in order to alleviate animal pain and distress.

USDA. ARS. National Agricultural Library.

The National Agricultural Library's Agricultural Thesaurus entry for "eye irritation tests" includes a list of related terms as well as links to simple keyword searches in Agricola and Google Scholar.

Search for Scientific Literature on Eye Irritation Testing

 

Click on the following resources for scientific literature about reduction, refinement or replacement (3Rs alternatives) in eye irritation testing.