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Rapid Detection of Eurasian and American H7 Subtype Influenza A Viruses Using a Single TaqManMGB Real-Time RT-PCR

Rapid Detection of Eurasian and American H7 Subtype Influenza A Viruses Using a Single TaqManMGB Real-Time RT-PCR.jpg
A real-time reverse transcription PCR (RRT-PCR) targeting a highly conserved HA2 H7 region was developed for the detection of all H7 subtype avian influenza viruses (PanH7). The wide phylogenetic scope and analytical sensitivity and specificity were validated with the use of a panel of 56 diverse influenza A viruses. The detection limit was…