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Application of Co-Culture and Simulated-Vaginal Models to Elucidate the Inhibitory Properties of Naturally Occurring and Bioengineered Strains of Lactobacillus Toward Toxic-Shock Syndrome Toxin-1 (TSST-1) Producing Strains of Staphylococcus aureus

Investigators
Hart, Mark
Institutions
DHHS/FDA - National Center for Toxicological Research
DHHS - Centers for Disease Control and Prevention (CDC)
Objective

  1. Determine the inhibitory effects of a select group of lactobacilli with probiotic potential on a wide variety of S. aureus TSST-1 producing strains isolated from patients with Toxic Shock Syndrome using previously developed co-culture system and the vaginal tract (toroid) model with recently developed genital tract secretion medium.
  2. Generate transcriptional and proteomic profiles of Lactobacillus sp. and S. aureus strains using previously developed co-culture system and identify gene systems and proteins critical for inhibition of S. aureus growth and/or TSST-1 production.
  3. Isolate and clone the lysostaphin gene, an endopeptidase that specifically cleaves the cell wall cross-linking pentaglycine bridges of S. aureus, into a select group of lactobacilli and determine expression levels of lysostaphin as well as inhibitory capacity of engineered lactobacilli against S. aureus in the co-culture system and the vaginal tract (toroid) model.
More information
Responsible Division: Microbiology
Funding Source
Nat'l. Center For Toxicological Research
Project source
View this project
Project number
E0728601
Categories
Parasites
Natural Toxins
Viruses and Prions
Bacterial Pathogens
Chemical Contaminants
Staphylococcus