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Applications of Novel Yam Starch Films Containing Antimicrobial Agents to Improve Beef Safety

Lin, Mengshi
University of Missouri - Columbia
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E. coli O157:H7 is one of the most important foodborne pathogens in the beef industry and has resulted in a number of highly publicized and expensive recalls. Traditional antimicrobial agents widely used in the beef processing plants include quaternary ammonium compound, chlorine, acetic acid, and others. Many of these acidic and alkaline sanitizers are corrosive, emit unpleasant odors, discolor surfaces, and are often vulnerable to harsh processing conditions and abusive factors during transport and storage (high temperature, high pressure, humidity), resulting in the loss of their antimicrobial properties due to decomposition or sublimation of the antimicrobial agents.

Novel inorganic antibacterial materials such as metal or metal oxides nanoparticles (NPs) have received increasing attention in recent years because they are non-corrosive, odorless, and stable under harsh processing conditions. Particularly, inorganic NPs made from metal oxides with sizes less than 100 nm exhibit antimicrobial activities due to their special characteristics such as small particle size and large surface area.

With recent advances in film production and technology, there is growing interest in the applications of novel antimicrobial films and coatings in microbial food safety. Yam (Dioscorea alata) starch can form a transparent, colorless, odorless, and flexible film which is an excellent oxygen and moisture barrier as a packaging material for food products.

Objectives: The objectives of this study were to evaluate antimicrobial properties of novel inorganic antimicrobial agents (zinc oxide nanoparticles (ZnO NPs) and calcium phosphate hydroxyapatite (CaHA NPs)) against an important foodborne pathogen, E. coli O157:H7; study the mode of action of NPs against bacteria and the development of bacterial biofilms on stainless steel; and develop and use yam starch films containing antimicrobial NPs to inhibit the growth of E. coli O157:H7 in beef products.

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Findings: ZnO NPs showed strong antimicrobial activities with increasing inhibitory effects on the growth of E. coli O157:H7 as the concentrations of ZnO NPs increased; while CaHA NPs show mild antimicrobial activities against E. coli O157:H7. ZnO NPs could be used to inhibit the development of E. coli O157:H7 biofilms on the surface of stainless steel and incorporated into yam starch films to control the growth of E. coli O157:H7 on sirloin beef cuts. Yam starch has long been used as a traditional medicine that provides great health benefits while ZnO have excellent biocompatibility and bioactivity and are stable under harsh processing conditions. Together, they could provide novel intervention strategies for pathogen control for beef products.
Funding Source
Nat'l. Cattlemen's Beef Assoc.
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Natural Toxins
Viruses and Prions
Bacterial Pathogens
Chemical Contaminants
Escherichia coli