An official website of the United States government.

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

An Appraisal of the Efficacy and Feasibility of Rapid, On-Farm Test Methods for the Detection of Campylobacter

Institutions
Agri-Food and Biosciences Institute (AFBI)
Start date
2011
End date
2012
Objective

This project will assess the feasibility of using a range of immuno- and nucleic acid-based detection assays to detect campylobacter in on-farm flock and environmental matrices.

A number of on-farm samples including litter, faeces (composite), dust, air, and dead bird ventral swabs will be evaluated to determine which matrix will yield the highest levels of campylobacter for presentation to detection assay.

It is not anticipated that liquid culture enrichment would take place on-farm in the ultimate test protocol due to health and safety risks to test users (farmers) and opportunities for cross-contamination.

Physical enrichment methods, including (immuno) magnetic separation, which are commercially available in convenient technology, will be assessed for sensitivity, specificity, ease of use and compatibility with detection devices. The outcomes of this early stage work will enable the highest number of campylobacter to be presented to a suitable detection assay.

Only rapid detection methods at/or close to commercial availability will be considered for feasibility of use on-farm. Development of new technologies will not be undertaken, however, where available and applied to other pathogens, the technologies will be developed for campylobacter. The primary methods considered in this feasibility study will be lateral flow device, loop-mediated isothermal amplification, an immuno-biosensor and real time PCR assays.

More information

Background: A rapid, robust, specific, sensitive test for campylobacter, suited for on-farm use, which enables results to be available in real time, would greatly assist on-farm control by enabling farmers to monitor the outcomes/impact/efficacy of on-farm intervention activities, on-farm management and on-farm biosecurity. Such a test may enable future development of a ‘test and schedule‘ programme, whereby chicken flocks shedding high numbers of campylobacter would be identified prior to transport and slaughter.

Funding Source
Food Standards Agency
Project source
View this project
Project number
FS241049A
Categories
Bacterial Pathogens
Prevention and Control
Campylobacter
Commodities
Meat, Poultry, Game