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APTAMER-ANTIBODY BASED IMMUNOASSAY FOR THE DETECTION OF CRUSTACEAN SHELLFISH ALLERGENS

Investigators
Rao, Q.
Institutions
Florida State University
Start date
2021
End date
2023
Objective
The long-term goal of this project is to provide a convenient, reliable, and effective analytical tool for the enforcement of the FDA Food Safety Modernization Act (FSMA) in order to prevent intentional or unintentional cross-contamination of crustacean shellfish allergens in foods. We will achieve this goal through the integration of three major supporting objectives:(1) Purify and characterize four major crustacean shellfish allergensfrom shrimp and crab;(2) Develop and characterize monoclonal antibodies (mAbs) and DNA aptamers specific to four major crustacean shellfish allergens; and(3) Develop and validate aptamer-antibody based sandwich immunoassays for the detection of crustacean shellfish allergens in foods.
Funding Source
Nat'l. Inst. of Food and Agriculture
Project source
View this project
Project number
FLAW-2020-03475
Accession number
1024151
Categories
Prevention and Control
Commodities
Seafood