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Assay Development for Detection of Pathogenic E. Coli in Environmental Samples

Investigators
Shelton, Daniel
Institutions
USDA - Agricultural Research Service
Start date
2008
End date
2009
Objective
The objective of this cooperative research project is to develop an assay for the detection of pathogenic E. coli in water, soil, manure and food matrices using an integrated immuno-PCR biosensor.
More information
Approach: Pathogenic E. coli will be captured and fluorescently labeled with specific monoclonal antibodies. The E. coli cell-antibody complex will subsequently be detected using an immunoassay biosensor followed by PCR characterization.
Funding Source
Agricultural Research Service
Project number
1265-63000-001-05
Categories
Escherichia coli
Prevention and Control