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BFPE Structure/Function in Type IV Pilus Biogenesis

Investigators
Blank, Eric
Institutions
University of Maryland - Baltimore Professional School
Start date
2000
End date
2000
Objective
A non-polar mutation in bfpE will be constructed, and the effect of this lesion on pilus production and bacterial adherence will be examined. A detailed topological analysis of BfpA will be carried out using phoA/lacZ reporter systems. The effects of mutations in the hydrophilic segments of BfpE will be determined to identify critical residues for pilus function. Three different methods: selection of suppressor of BfpE, cross linking, and use of the two-hybrid system, are proposed to study the interaction of BfpE with other proteins.
More information
The BfpE protein of enteropathogenic E. coli (EPEC) is a representative of a family of cytoplasmic membrane proteins that participate in the biogenesis of type IV pili. It is hypothesized that BfpE is directly required for pilus formation and that it is a key component of a protein complex encoded by the bfp gene cluster. Experimental strategies are proposed to gain a better understanding of the structure and function of BfpE.

The results of the proposed research should advance our knowledge about the mechanisms involved in the biogenesis of EPEC pili.

Funding Source
Nat'l. Inst. of Allergy and Infectious Diseases
Project number
5F32AI010191-03
Categories
Escherichia coli