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Bioluminescence as a tool for the detection and evaluation of pathogen contamination of food

Investigators
Applegate, Bruce
Institutions
Purdue University
Start date
2019
End date
2024
Objective
The proposed program will focus on improving the detection of problematic foodborne pathogens to both allow increased surveillance and reduced times for detection. The program will also use bioluminescence as a tool to evaluate parameters associated with fresh produce contamination as well as parameters affecting attachment and shedding of E. coli and Salmonella associated with meat production.Construction/isolation of a battery of genetically modified lysogenic bacteriophage for the detection of E. coli STEC strains O26:H11, O103:H2, O111:NM, O121:H19, O45:H2, O145:NM, Campylobacter spp. including C. jejuni and C. coli, Listeria monocytogenes, and problematic Salmonella serovars and development of selective enrichment protocols.Use previously developed bioluminescent strains of the relevant foodborne pathogens Salmonella spp., Escherichia coli STEC strains, and Listeria monocytogenes to examine key parameters associated with produce contamination.Use of bioluminescent Escherichia coli O157:H7 strains and Salmonella spp. to examine physiological conditions affecting the colonization of porcine and bovine intestinal tracts.
Funding Source
Nat'l. Inst. of Food and Agriculture
Project source
View this project
Project number
IND90025970
Accession number
1019795
Categories
Bacterial Pathogens
Escherichia coli
Commodities
Meat, Poultry, Game