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Biosensor Methods for "Realtime" Detection of Pathogens and Toxins

Start date
2000
End date
2000
Objective
SEA activity as a superantigen will be quantitated in vitro, using T-cell. T-cell stimulation will be measured as cell proliferation, apoptosis and induction of cytokines,adapting nonradioactive assays. Physical characterization of SEA will be carried out using Western blotting, chromatographic and electrophoretic separations and spectroscopy.
Funding Source
Center For Food Safety and Applied Nutrition
Project number
28660
Categories
Prevention and Control
Natural Toxins
Predictive Microbiology