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Biosensor Processes For Detecting Pathogenic Bacteria In Foods

Investigators
Brewster, Jeffrey; Irwin, Peter; Gehring, Andrew
Institutions
USDA - Agricultural Research Service
Start date
2001
End date
2006
Objective
The specific objectives of this research are to develop:
  1. Rapid and effective methods to separate and concentrate various pathogens from food samples in order to shorten the duration of, or reduce the need for, culture enrichment;
  2. Simple and effective sensors for the screening detection of food-borne pathogens;
  3. Integrated detection procedures suitable for regulatory and/or industrial applications.
More information
To develop the means to select and concentrate only target organisms in the presence of an elaborate non-pathogenic microflora. We propose to develop both IMB and immuno-affinity column technologies to more rapidly concentrate various food-borne target pathogens and thereby shorten the time period of, and minimize the need for, predetection culture enrichment.

Biosensor methods will also be developed including, but not limited to:

  1. An alkaline phosphatase-antibody system suitable for 96-well microplate readers;
  2. Metabolic control of cellular ATP level to confirm the presence of viable cells;
  3. The use of the streptavidin-biotin interaction to enhance sample stability for light addressable potentiometric detection;
  4. A cytolethal distending toxin (CDT) - mediated membrane disintegration assay; and
  5. A liquid crystal detection system.

Lastly, we propose to integrate and combine the most promising of these procedures into one, or more, screening protocols for regulatory and/or industrial applications.

Funding Source
Agricultural Research Service
Project number
1935-42000-046-00D
Accession number
404750
Categories
Natural Toxins
Microbiological Standards and Guidelines
Prevention and Control