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BREEDING OF BROCCOLI FOR MUCH ENHANCED RESISTANCE TO MULTI-ABIOTIC STRESSES WITH SIGNIFICANTLY PROLONGED POSTHARVEST LIFE

Investigators
Gan, Su.; Griffiths, Ph, .
Institutions
Cornell University
Start date
2020
End date
2022
Objective
This proposed research is closely related to the emphasis areas of "Agricultural Environmental Management", "Food Safety and Producers/Processors/Retailers/Food Service Providers" and "Climate change". The overall objectives are to produce new broccoli germplasm that will be much resistant to multi-abiotic stresses such as drought, salt, heat, cold, and darkness with significantly prolonged postharvest life. The specific research objectives are as follows:1. To identify BoNAP gene;2. To design target sequences for genome editing in broccoli using CRISPR/CAS9 system;3. To establish broccoli transformation system and use the system to transform broccoli with the constructed CRISPR/CAS9 plasmids;4. To identify broccoli lines with BoNAP sequence edited (bonap knockouts) by sequencing target genomic DNA of the transformed broccoli.
Funding Source
Nat'l. Inst. of Food and Agriculture
Project source
View this project
Project number
NYC-184404
Accession number
1024038