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Campylobacter Epidemiology, Methods Development & Interventions In Poultry

Investigators
Cox, Nelson; Hiett, Kelli; Line, John; Seal, Bruce
Institutions
USDA - Agricultural Research Service
Start date
2001
End date
2006
Objective
  1. Collect data for a poultry risk assessment model describing the effects of production, transport and processing on Campylobacter contamination.
  2. Identify sources (by DNA sequencing) in poultry operations.
  3. Assess and characterize the potential for vertical spread and determine value in hatchery intervention.
  4. Define antagonistic flora. Create detection assay to selectively isolate and enumerate.
More information
  1. Obtain and quantitatively analyze Campylobacter in poultry operations and, compare with human disease causing strains to create a risk assessment model.
  2. Cooperatively with the U.S. poultry industry, assess the magnitude of breeder transmission to the broilers and determine benefit of hatchery interventions. Use DNA sequencing for tracking.
  3. Conduct experimentation to determine etiology of breeder/broiler transmission.
  4. Screen and identify antagonistic bacteria.
  5. Using biochemical characteristics, create selective/differential media for use by Regulatory Agencies.
  6. Screen and identify antagonistic bacteria. Select conserved DNA components for creation of a field friendly PCR assay.
Using this template, create an RT PCR assay that detects only live cells. Research in the processing plants will be carried out cooperatively with other appropriate ARS management units.
Funding Source
Agricultural Research Service
Project number
6612-32000-034-00D
Accession number
404666
Categories
Prevention and Control
Risk Assessment, Management, and Communication
Campylobacter