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Characterisation of the Nature of Combined Actions of Chemical Mixtures and Estimation of Dose Response Relationships for Pesticide Mixtures

Institutions
Central Science Laboratory
Start date
2006
End date
2008
Objective
This research project aims to characterise potential interactions of mixtures of anti-acetylcholinesterase pesticides.

Experimental work will be carried out in vitro using the human neuroblastoma cell line SH-SY5Y. Cells will be exposed to different concentrations (doses) of single compounds, an equimolar ratio cocktail and a cocktail containing relative concentrations that are proportional to those likely to arise from dietary exposure.

The toxicity of the pesticides (two organophosphates and two carbamates) will be measured as inhibition of acetylcholinesterase activity.

Appropriate statistical methods will be used to test a null hypothesis that the exposure-response relationship for mixtures along each fixed-ratio ray is the same as that predicted by combining (adding) the effects of the individual chemicals.

The outcome from this project will not only provide an answer to the direct question of interaction between four anti-cholinesterase pesticides, but also provide a model for future study of more complex food contaminant cocktails.

More information
In its report on Risk Assessment of Mixtures of Pesticides and Similar Substances, the Committee on Toxicity of Chemicals in Food Consumer Products and the Environment (COT) recommended a need for investigation of the nature and dose-response relationships for combined actions of pesticides.

This project is designed to answer the specific question of whether anti-acetylcholinesterase pesticides are simply additive in their effect at different doses or if there is a more complex interaction (synergism or antagonism).

Find more about this project and other FSA food safety-related projects at the Food Standards Agency Research webpage.

Funding Source
Food Standards Agency
Project number
T10016
Categories
Sanitation and Quality Standards
Bacterial Pathogens
Natural Toxins
Risk Assessment, Management, and Communication