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Characterization of Endothelial Cell Internalization of AP4A

Investigators
Hilderman, Richard
Institutions
Clemson University
Start date
1999
End date
2004
Objective
  1. Characterization of Ap4A interaction with the low affinity binding site on bovine aortic endothelial cells surface;
  2. Characterization of the Ap4A internalization process;
  3. Characterization of Ap4A interaction with the endoplasmic reticulum;
  4. Characterization of internalized Ap4A induction of nitric oxide synthesis.
More information
We will determine whether Ap4A is internalized by binding to the low affinity binding site on the bovine aortic endothelial cell surface by characterizing the effects of P2 agonists, P2 antagonists and various guanosine dinucleotides on Ap4A internalization. The P2 agonists, P2 antagonists and guanosine dinucleotides only interact with the low affinity binding site. The mechanism of Ap4A internalization will be accomplished by determining whether protease activation and/or macromolecular biosynthesis are required for internalization. We will also determine whether Ap4A is internalized by an endocytic or transporter system. We will determine whether Ap4A binding to endoplasmic reticulum induces the release of calcium from calcium release channels. This will be accomplished by characterizing the binding of Ap4A to endoplasmic reticulum and also by determining whether Ap4A induces calcium release from endoplasmic reticulum receptors. To determine whether the internalization of Ap4A induces the synthesis of nitric oxide we will determine whether the internalization of Ap4A induces nitric oxide synthesis in the presence and absence of inhibitors of nitric oxide formation.
Funding Source
Nat'l. Inst. of Food and Agriculture
Project source
View this project
Project number
SC-1700108
Accession number
182274