An official website of the United States government.

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Comparative genomics analysis and physiological assessment of the avirulent Salmonella surrogate relevant to produce safety

Investigators
Moraes, Marcos; Teplitski, Max
Institutions
University of Florida
Start date
2016
End date
2017
Objective
Coliforms and generic E. coli are poor predictors of the behavior of human pathogens (like Salmonella, pathogenic E. coli and Listeria) in the crop production environment. Mounting evidence suggests that accurate models of Salmonella behavior in the production environment will have to be built based on the experiments conducted with Salmonella, and not based on data from distantly related surrogates like generic E. coli. This, however, necessitates availability and careful characterization of “disarmed” strains of Salmonella that could be used for on-site research. Upon completion of this study we will have developed robust tools for modeling behavior of these outbreak strains in the pre- and post-harvest production environments. The purpose of this project is to carry out comparative genomic and physiological characterization of the outbreak strains under production conditions and to compare them with the nonvirulent strain of Salmonella that we have developed. We will also have tested two key hypotheses aimed at understanding why only a dozen out of over 2,500 Salmonella serovars are associated with produce-linked outbreaks of illness. With previous CPS funding we engineered and verified the first nonvirulent, nontransgenic strain of Salmonella suitable for on-site studies as an indicator organism.
Funding Source
Center for Produce Safety
Project source
View this project
Project number
2016-443
Categories
Escherichia coli
Listeria
Salmonella
Parasites
Natural Toxins
Viruses and Prions
Bacterial Pathogens
Chemical Contaminants