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Comparison of MALDI TOF MS and MALDI FTMS for Typing Vibrio Bacteria

Investigators
Lay, Jackson
Institutions
DHHS/FDA - National Center for Toxicological Research
Objective
The principal investigator developed the pilot studies for this proposal by transporting mass spectrometry combined with pattern recognition technology to the CFSAN:
  1. The biology of learning sets is required to develop a test system.
  2. Simple membrane-fractionation techniques will enhance numbers of identified proteins.
  3. Sub-typing of Vibrio parahemolyticus is transportable to other systems.
  4. The method can sub-type pathogenic from non-pathogenic E. coli strains.
  5. A joint FDA/IAOAC symposium to discuss the role of mass spectrometry in the rapid speciation of bacteria (June 2001).
More information
FY 2001 Plans:
Complete development of collaborative studies among personnel and equipmentlocated at CFSAN, NCTR and the University of Arkansas at Fayetteville, AR: 1) Evaluation of transport of technology to simpler TOF instruments. 2) Evaluation of transport of technology to MALDI utilizing FT (Fourier Transform) enabling high-resolution detection in larger molecular weight ranges. It is anticipated that one two-week assignment each quarter to CFSAN and preliminary experiments at the University of Arkansas at Fayetteville will enable these experiments.
Funding Source
Nat'l. Center For Toxicological Research
Project number
X10009
Categories
Legislation and Regulations
Escherichia coli
Vibrio