An official website of the United States government.

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Controlling the Growth of Human Pathogens on Fresh Fruits and Vegetables

Investigators
Bhagwat, Arvind; Angle, John; Gross, Kenneth
Institutions
University of Maryland - College Park
Start date
2001
End date
2006
Objective
The objectives of this proposed research will be to gain a greater understanding of the biochemical and molecular genetic mechanisms involved in the attachment,survival and growth of foodborne pathogens on fresh fruits and vegetables.
More information
Our cooperative approach will be to standardze isolation protocols for beta-glucans from foodborne human pathogens, based on our past experience with Rhizobium and E. coli strains. Perform structural characterization various carbohydrate analyses techniques such as gas chromatography, mass spectrometry, and NMR. Examine regulatory factors influencing synthesis of beta-glucans using in vitro and in vivo assays. Generate mutants of Salmonella and E. coli O157:H7 strains defective in beta-glucan synthesis. Finally, we will study the attachment, growth and survival of mutant (and wild type) strains on fresh and fresh-cut produce.
Funding Source
Agricultural Research Service
Project number
1275-42000-002-04S
Categories
Salmonella
Escherichia coli