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Decontamination of Alfalfa Seeds and Sprouts with Ozone

Investigators
Fett, William
Institutions
South Dakota State University
Start date
2000
End date
2002
Objective
Develop methods to decontaminate alfalfa seed and sprouts of the human pathogens Escherichia coli O157:H7 and Listeria monocytogenes by treatment with ozone.
More information
Ozonated water will be generated in the laboratory and various modes of introduction will be studied for decontamination of alfalfa seed laboratory-inoculated with pathogen cocktails containing either E. coli O157:H7 or L. monocytogenes. Various concentrations (2,4,6,8 and 10 mg/L) of ozone, various contact times (1,2,4,8 and 16 min) and the effect of agitation of treatment efficacy will be evaluated. The effect of ozone treatment on the formation and viability of biofilms on sprout surfaces will be examined by use of a confocal scanning laser microscope. Technologies will be transferred to sprout growers through the International Sprout Growers Association, Amherst, MA, and through technical publications.
Funding Source
Agricultural Research Service
Project number
1935-41420-006-01S
Categories
Escherichia coli
Listeria
Bacterial Pathogens
Commodities
Produce