An official website of the United States government.

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Detection and Safe Management of Microorganisms in Poultry and Swine Wastes

Investigators
Jenkins, Johnie
Institutions
USDA - Agricultural Research Service
Start date
2005
End date
2008
Objective
Develop laboratory systems to determine the bacterial profile (quantity and types of bacteria present in manure, soil, plant, or air) as affected by management system, environment, and time. Isolate bacteriophages for Campylobacter, Salmonella spp., and Yersinia as indicators of bacterial presence and use in control of pathogens. Determine soil chemical properties which impact fate of harmful manure pathogens.
More information
Approach: Develop mechanical system for repeatedly photographing and measuring, over time, the same plated colony on inoculated plates using a rotating stage and position tracking with computer program. Using different culture media and dilutions and spiral inoculated plates, microbial profile changes are determined of gram-negative enteric bacteria as a function of management systems applied in CRIS 6406-12630-003-00D. Samples of swine lagoon effluent and poultry litter are tested for presence of bacteriophages using bacterial 'lawns' of American Type Culutre Collecting in a double-agar-layer plaque assay. Phage is diluted to characterize individual isolates using electron microscopy and standard techniques. Spiked samples and natural manure samples are applied to four well-characterized soils in percolation column studies and in runoff studies. Microbial profiles are determined in water samples collected with simulated rain events as a function of time, temperature, soil characters, and, where appropriate, soil depth.
Funding Source
Agricultural Research Service
Project number
6406-32000-009-00D
Accession number
409548
Categories
Salmonella
Campylobacter
Commodities
Meat, Poultry, Game