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Detection of Cytolethal Distending toxin (cdtB), pltA and pltB Homologs of Components of the Pertussis Toxin Genes by PCR and Studies on Functionality of cdtB in Non-Typhoidal Salmonella spp.

Investigators
Khan, Ashraf
Institutions
DHHS/FDA - National Center for Toxicological Research
Start date
2015
End date
2016
Objective

 

  1. Develop a duplex real-time, quantitative polymerase chain reaction (qPCR) to detect Salmonella and antibioticresistance gene markers simultaneously from food samples.
  2. Validation of qPCR method at FDA, ORA laboratories.
  3. Characterize plasmids, virulence genes, and integrons, for their role in virulence in multidrugresistant Salmonella strains isolates from food.

 

More information
Responsible NCTR Division: Microbiology
Funding Source
Nat'l. Center For Toxicological Research
Project source
View this project
Project number
E0739601
Categories
Bacterial Pathogens
Natural Toxins
Viruses and Prions
Salmonella