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Development and Validation of Standard Operating Procedures for Measuring Microbial Populations for Estimating a Postmortem Interval

Investigators
Crippen, Tawni
Institutions
USDA - Agricultural Research Service
Start date
2010
End date
2013
Objective
To determine the practicality and usability of biochemical analysis and pyrosequencing technologies for determining bacterial succession on decomposing remains (e.g. animal carcasses, waste management). We will validate the standard operating procedures for sampling and analyzing microbe populations, as it relates to animal production and consequently food safety.
More information
Approach: Use molecular methods to characterize microbial succession and assess microbial–arthropod interactions for understanding insect arrival sequence, utilization, and relevant bacterial dispersal potential; assess arthropod influence on microbial communities and microbial mediation of arthropod behavior on decomposing resources. These techniques will help assess the influence of microbial communities on decomposing carcasses and the effect of waste management practices in food animal production.
Funding Source
Agricultural Research Service
Project source
View this project
Project number
6202-32000-032-55R
Accession number
420562
Categories
Sanitation and Quality Standards
Parasites
Natural Toxins
Viruses and Prions
Bacterial Pathogens
Chemical Contaminants