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Development of Rapid Tests for the Detection of Mycobacterium Avium subsp. Paratuberculosis (MAP) and Their Use to Determine Survival in Food Matrices

Institutions
Queen's University - Belfast
National University of Ireland, Galway (NUI Galway)
Start date
2001
End date
2004
Objective
  1. Optimisation of a PCR/DNA probe assay for the detection of MAP in faeces for clinical and sub-clinically infected animals.
  2. Optimisation of sample preparation methods to enable the sensitive application of the PCR/DNA probe assay for the detection of MAP in faeces
  3. Validation of the application of the assays for the identification of MAP in infected/suspect heerds.
  4. Optimization of a NucliSens assay for the detection of MAP RNA in milk as a monitor for the presence of viable organisms in milk.
  5. Determination of the heat resistance of MAP in milk.
More information
Progress: Monclonals have been raised but further work is required to find the most suitable for further development. Data has been obtained on heat resistance but as yet insufficient to complete a response surface model. A RT-PCR assay has been developed but further work is required to optimize the method. A real time assay for MAP has been developed but its value has still to be validated using naturally infected samples.

Funding Source
safefood
Project number
00-RESR-060