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The Development of Transgenic Mice Harboring Bacteriophage 0X174 with Sites Specific for Detecting Mutations at Guanosine:Cytosine Nucleotides, Small Frameshifts, and Extended Deletions

Investigators
Valentine, Carrie
Institutions
DHHS/FDA - National Center for Toxicological Research
Objective
To find specific mutations in bacteriophage Å"ÃœX174 that render the bacteriophage non-infectious and that will revert to plaque-forming ability only when mutation occurs by specific mechanisms: 1. Base substitution at a G:C base pair or 2. Frameshift caused by deletion of one or two nucleotides. An additional objective is to determine the feasibility of using Å"ÃœX174 to detect 3. The deletion of an extended sequence. Phage harboring these mutations will be used to construct a transgenic mouse model for measuring mutations in vivo.
Abstract
To find specific mutations in bacteriophage 0X174 that render the bacteriophage non-infectious and that will revert to plaque-forming ability only when mutation occurs by specific mechanisms: 1) base substitution at a G:C base pair or 2) frameshift caused by deletion of one or two nucleotides. An additional objective is to determine the feasibility of using 0X174 to detect 3) the deletion of an extended sequence. Phage harboring these mutations will be used to construct a transgenic mouse model for measuring mutations in vivo.
Funding Source
Nat'l. Center For Toxicological Research
Project number
E0700201
Categories
Sanitation and Quality Standards