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DNA-Based Typing of Staphylococcus aureus Associated with Human and Animal Diseases

Investigators
Kloos, W. E.
Institutions
North Carolina State University
Start date
2019
End date
2004
Objective
The main objective of the project is to identify strains of Staphylococcus aureus which produce specific types of infections in humans and animals, especially those that demonstrate a propensity to colonize certain organs and tissues. The specific objectives are 1) determine the pulsed-field gel electrophoresis (PFGE) and ribotype chromosome fragment patterns of S. aureus isolates taken from patients with septicemia and a specified type and primary site of infection, 2) determine the PFGE and ribotype chromosome fragment patterns of S. aureus isolates taken from dairy cows and goats with mastitis, 3) identify S. aureus ecovar-specific chromosome fragments for the purpose of distinguishing human, bovine, and goat strains from each other, and use this information to assess transfer between host species, and 4)determine the persistence of S. aureus strains causing mastitis within infected dairy herds and their movement between herds.
Funding Source
Nat'l. Inst. of Food and Agriculture
Project source
View this project
Project number
NC06593
Accession number
186764
Categories
Bacterial Pathogens
Staphylococcus
Commodities
Meat, Poultry, Game