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Enumeration of Salmonella enterica in meat samples with Droplet Digital PCR

Investigators
Dickson, James; Logue, Catherine M
Institutions
Iowa State University
Start date
2014
End date
2015
Objective
Several different serovars of Salmonella enterica, corresponding to those commonly found in meat products based on the USDA-FSIS quarterly reports on Salmonella serotyping, will be enumerated at different populations using digital droplet PCR, and compared to conventional surface plate enumeration methods. The experiments will be repeated using a mixed microflora, using purge from vacuum-packaged beef as a source of the non-pathogenic bacteria. Ground beef with different fat contents will be inoculated with individual strains of Salmonella. The target populations of Salmonella will range from 2 cells/10 grams to 1000 cells per gram. The samples will be enumerated by both DDPCR and standard methods, and a comparison will be made between the two methods to establish the sensitivity of the DDPCR method.
Funding Source
Fndn. for Meat and Poultry Research and Education
Project source
View this project
Project number
13-314
Categories
Legislation and Regulations
Salmonella
Packaging Residues