- Investigators
- Dickson, James; Logue, Catherine M
- Institutions
- Iowa State University
- Start date
- 2014
- End date
- 2015
- Objective
- Several different serovars of Salmonella enterica, corresponding to those commonly found in meat products based on the USDA-FSIS quarterly reports on Salmonella serotyping, will be enumerated at different populations using digital droplet PCR, and compared to conventional surface plate enumeration methods. The experiments will be repeated using a mixed microflora, using purge from vacuum-packaged beef as a source of the non-pathogenic bacteria. Ground beef with different fat contents will be inoculated with individual strains of Salmonella. The target populations of Salmonella will range from 2 cells/10 grams to 1000 cells per gram. The samples will be enumerated by both DDPCR and standard methods, and a comparison will be made between the two methods to establish the sensitivity of the DDPCR method.
- Funding Source
- Fndn. for Meat and Poultry Research and Education
- Project source
- View this project
- Project number
- 13-314
- Categories
- Legislation and Regulations
- Salmonella
- Packaging Residues
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