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The Evaluation of the Genotoxicity and Concentration-Response Relationships of the Azo Dyes Sudan 1 and Para Red

Institutions
University of Wales - Swansea
Start date
2006
End date
2006
Objective
This research project seeks to evaluate the genotoxicity of azo dyes and to investigate whether the genotoxicity occurs via a threshold mechanism

This project will employ methodologies developed as part of another FSA research project (T01029 – The evaluation of the existence of thresholds of activity of genotoxic substances).

Using cultured human lymphoblastoid cells, azo dyes will be assessed for their ability to induce gene mutations (at TK and HPRT loci) and chromosomal structural and numerical changes.

Protocols will also be modified to allow assessment of effects of potential biotransformation of the azo dyes.

Emphasis will be placed on fully investigating the shape of the genotoxicity dose-response relationship in order to establish if this effect exhibits a threshold.

Studies will also investigate potential interactions of combined exposure to azo dyes and other genotoxins with known mechanisms of action.

More information
The discovery of non-permitted azo dyes in food has highlighted the paucity of toxicological data relating to this class of chemicals.

In the first instance, this project will determine whether two azo dyes Sudan 1 and Para Red induce gene or chromosome mutations and, if so, particular emphasis will be placed on establishing the shape of the dose-response relationships for these effects.

Find more about this project and other FSA food safety-related projects at the Food Standards Agency Research webpage.

Funding Source
Food Standards Agency
Project number
T01038
Categories
Sanitation and Quality Standards
Natural Toxins
Preventive Food Safety Systems