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Evaluation of a PCR Assay for PRRS Virus in Saliva

Institutions
Veterinary Laboratories Agency, UK
Newcastle University
Start date
2009
End date
2010
Objective

Aims and objectives:

  • To investigate the potential of porcine oral fluid samples as a means of diagnosing and monitoring infection with PRRSv in UK pigs.

The project aimed to address the following aspects of oral fluid diagnostics in UK pigs:

  • Optimal methods of rope presentation, sample collection and handling
  • Some assessment of pig responses to the rope; factors affecting interactions with the rope, some idea of the proportion of pigs contributing to the sample
  • Optimisation of the technique for PRRSv RNA detection
  • Identification of problems encountered, to inform further research in this field.
Funding Source
Agriculture & Horticulture Develop. Board
Project source
View this project