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On Farm Ecology of Listeria monocytogenes

Investigators
Lindsay, James
Institutions
USDA - Agricultural Research Service
Start date
2003
End date
2007
Objective
  1. Determine the prevalence and genotypic and phenotypic characteristics of L. monocytogenes strains present in Pacific Northwest dairy farms.
  2. Use microarray technology to rapidly and accurately subtype L. monocytogenes isolates for use in both epidemiological studies and gene discovery.
  3. Use microarray and PFGE subtyping to examine on-farm ecology of L. monocytogenes and to compare on-farm subtypes to those obtained from human listeriosis cases.
More information
Preliminary microarray subtyping data indicate that a relatively simple mixed genome DNA microarray is capable of reconstructing previously described phylogenetic relationships between major serogroups and PFGE classifications.

Importantly, probes unique to each microarray and serotypic cluster were also identified. An expanded microarray has been used to increase subtyping resolution and identify a greater number of informative probes. A condensed micorarray consisting entirely of unique, informative probes will be constructed and used to study on-farm ecology and epidemiology of L. monocytogenes. The phenotypic importance of potentially virulence-associated probes will be accessed via gene knock out and infection of an in vitro or in vivo model. Formerly 5348-32000-017-00D (12/02). BSL- 1; 9/4/01.

Funding Source
Agricultural Research Service
Project number
5348-32000-022-00D
Categories
Bacterial Pathogens
Listeria