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FUSARIUM WILT OF COTTON: PATHOGEN POPULATION CHARACTERIZATION, VIRULENCE/ RESISTANCE MECHANISM DETERMINATION & MARKER ASSISTED RESISTANCE BREEDING

Investigators
Liu J
Institutions
USDA - Agricultural Research Service
Start date
2019
End date
2019
Objective
1.Characterize genetic variation, virulence, and nematode interactions of F. oxysporum isolates. 2. Advance and evaluate tolerance to FOV4 from ¿Pima S-2¿, ¿Pima S-4¿, ¿Pima S-6¿, ¿Sakel¿, ¿Seabrook Sea Island 12B-2¿, and Gossypium arboreum accession #190 into BAR 32 lines that are homozygous for Ren2,3 and Mi1,2 and are highly resistant to both root-knot and reniform nematodes. Identify markers associated with resistance. 3. Identify FOV4 virulence genes affected by transposon ¿Tfo1¿ and other mutator-like elements, and confirm the virulence role of the identified gene by gene knock-outs subsequent pathogenicity assays with knock-outs. 4. Determine the importance of fusaric acid in virulence and competition with antagonists using pathogenicity assays of various knock-out mutants in root rot pathotype isolates of Fov race 4 and Australian biotype.
Funding Source
Agricultural Research Service
Project source
View this project
Project number
3091-22000-035-59R
Accession number
436189
Categories
Natural Toxins
Viruses and Prions
Mycotoxins
Bacterial Pathogens