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Genetic and Structural Characterisation of Novel N-Linked General Glycosylation Systems in Eubacteria

Institutions
London School of Hygiene and Tropical Medicine
Start date
2004
End date
2007
Objective
We recently characterised a novel N-linked general glycosylation system (gss) from the epsilon-proteobacterium Campylobacter jejuni, that has several eukaryotic features (Wacker et al., Science, vol 298, p 1790). We have now identified a second such pathway, with five additional genes, in the related epsilon-proteobacterium Wolinella succinogenes (Wsggs). We cloned the entire pathway into E. coli to produce recombinant glycoprotein.

In this research programme, we aim to characterise the complex Wsggs by mutagenesis and structural analysis, and to identify and characterise further ggs pathways in epsilon-proteobacteria. Characterising this new family of glycosylation systems will facilitate glycoengineering, with broad research and industrial applications.

Funding Source
Biotechnology and Biological Sciences Research Council
Project number
BBS/B/04862
Categories
Campylobacter
Escherichia coli