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Genetic Engineering of Peanut for Reduction of Aflatoxin Contamination

Institutions
University of Alabama
Start date
1996
End date
2001
Objective
  1. Produce multiple independent transgenic cell lines and plants of peanut by microprojectile bombardment of embryogenic tissues cultured in vitro.
  2. Test for tissue specific expression of genes driven by tissue specific gene promoters in transformed peanut.
  3. Test peanut tissues transformed with fungal resistance genes for resistance to Aspergillus flavus/parasiticus.
More information
Embryogenic plant cell lines will be bombarded with DNA (containing genes for kanamycin or hygromycin resistance as selective markers) and stably transformed peanut tissues selected for antibiotic resistance. The tissue/organ specific expression of a beta-glucuronidase reporter gene driven by a soybean vegetative storage protein gene promoter will be tested in transgenic Arachis hypogaea progeny of the T1 and T2 generations.Other promoter/reporter or promoter/antifungal gene combinations will be similarly tested. As fungal resistance genes become available from cooperators, these genes will be tested for their effectiveness in inhibiting growth of Aspergillus flavus/parasiticus in peanut tissues transformed with these genes.
Funding Source
Agricultural Research Service
Project number
6435-42000-015-04S
Categories
Mycotoxins
Antimicrobial Resistance
Commodities
Nuts, Seeds