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Genomic and Metabolomic Approaches For Detection and Control of Fusarium, Fumonisins and Other Mycotoxins On Corn

Investigators
Proctor, Robert; Peterson, Stephen; O'Donnell, Kerry; Naumann, Todd; Brown, Daren
Institutions
USDA - Agricultural Research Service
Start date
2016
End date
2021
Objective

Objective 1: Use comparative phylogenomic approaches to enable accurate identification of mycotoxigenic Fusarium and to elucidate components of Fusarium genomes that are responsible for variation in mycotoxin production. Sub-objectives 1.1 through 1.3 are as follows: 1.1 ? Develop a DNA sequence database that facilitates accurate identification of all toxigenic Fusarium species; 1.2 ? Determine whether mycotoxin biosynthetic gene clusters and genetic networks that regulate cluster expression differ in their distributions among Fusarium species; 1.3 ? Determine whether F. verticillioides has genes that repress fumonisin production. Objective 2: Develop and utilize liquid chromatography-mass spectrometry (LC-MS) approaches for metabolomic analysis of Fusarium verticillioides infection of maize. Sub-objective 2.1 and 2.2 are as follows: 2.1 ? Develop workflows for untargeted analyses of the metabolomes of maize, F. verticillioides, and the maize-F. verticillioides interaction; and 2.2 ? Identify metabolic biomarkers for high and low levels of F. verticillioides-induced disease in maize. Objective 3: Identify and characterize plant and fungal factors that can impact mycotoxin contamination via their effects on plant disease development. Sub-Objective 3.1 through 3.4 are as follows: 3.1 ? Determine how primary sequence and secondary structure of fungal polyglycine hydrolases affect the inhibitory activity of this class of proteases against plant chitinases; 3.2 ? Isolate and identify ChitA alloform-specific proteases secreted by the fungi Stenocarpella maydis and Trichoderma viride; 3.3 ? Elucidate the role of plant class IV chitinases in maize-fungus interactions; and 3.4 ? Identify candidate receptor and regulatory genes that mediate oxylipin-induced changes in expression of fumonisin biosynthetic genes and fumonisin production in F. verticillioides. Objective 4: Identify and characterize components of fungus-fungus interactions that contribute to or inhibit mycotoxin contamination of crops. Sub-objective 4.1 through 4.3 are as follows: 4.1 ? Sample across different climate zones to identify novel fungal endophytes of maize that inhibit growth and/or fumonisin production in F. verticillioides; 4.2 ? Identify candidate genes in Talaromyces that are responsible for inhibition of growth in F. verticillioides; and 4.3 ? Determine whether production of fumonisins and other mycotoxins contributes to the competitiveness of F. verticillioides with other Fusarium species.

Funding Source
Agricultural Research Service
ARS (NP108)
Project source
View this project
Project number
5010-42000-050-00D
Accession number
430343
Categories
Natural Toxins
Mycotoxins