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Genotypic Subtyping of Multiresistant Salmonella Typhimurium DT 104 from Food Animals and Humans

Veterinary Laboratories Agency, UK
Health Protection Agency
Start date
End date
This research project aims to compare techniques to identify strains of salmonella, this is will help in tracing human infections back to their source.

This project aimed to compare a number of molecular techniques e.g. plasmid profiling, pulsed field gel electrophoresis, fluorescent amplified fragment length polymorphisms and sequence polymorphisms in a gene conferring resistance to the antibiotic ciprofloxacin to investigate whether a combination of the methods could subdivide S. typhimurium DT104 strains more effectively.

More information
In the tracing of human infections back to their source e.g. from the manufacturing environment, farm etc., it is necessary to be able to separate bacterial species or strains into a sufficiently large number of subtypes that the offending organism can be identified with a reasonable degree of certainty.

At the beginning of this project the methods in use to sub-divide salmonella phage types when applied to S. typhimurium DT104 placed more than 95% of the isolates in a single group.

Find more about this project and other FSA food safety-related projects at the Food Standards Agency Research webpage.

Funding Source
Food Standards Agency
Project number