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Immunobiology of Walnut Food Allergy

Investigators
Teuber, Suzanne
Institutions
University of California - Davis
Start date
1999
End date
2003
Objective
The major thesis of this proposal is that plant seed proteins, because of the way they are packaged as whole proteins in the plant protein body storage organelle with associated lectins, enzymes, and polyphenolic compounds, are able to stimulate the APC in atopic persons to modulate the cytokine milieu towards increased IL-4 and IL-13, inducing an IgE response.

As a prototype seed to study, the walnut (Juglans regia) will be used based on the availability of human subjects, recombinant allergens, multiple protein preparations, fractionated polyphenolics and its importance as a tree nut allergen. To characterize the APC-T cell interaction, T cell lines will be established from individuals with walnut food allergy and individuals with atopic dermatitis without food allergy. The proliferative response and Th2 related cytokine mRNA transcription will be assessed in response to different antigen packages delivered to the APC: recombinant Jug r 1 and Jug r 2, peptide fragments, whole purified proteins (albumins and large globulins), purified protein bodies (lectins and enzymes present), total walnut extract (pellicle polyphenolics and oil body lipids present), and the above protein sources with a quantified walnut total polyphenolic fraction added (rich in quercetin and ellagic acid).

The above data will significantly advance our knowledge of the immunobiology of plant seed allergy.

More information
The major thesis of this proposal is that plant seed proteins, because of the way they are packaged as whole proteins in the plant protein body storage organelle with associated lectins, enzymes, and polyphenolic compounds, are able to stimulate the APC in atopic persons to modulate the cytokine milieu towards increased IL-4 and IL-13, inducing an IgE response. As a prototype seed to study, the walnut (Juglans regia) will be used based on the availability of human subjects, recombinant allergens, multiple protein preparations, fractionated polyphenolics and its importance as a tree nut allergen. To characterize the APC-T cell interaction, T cell lines will be established from individuals with walnut food allergy and individuals with atopic dermatitis without food allergy. The proliferative response and Th2 related cytokine mRNA transcription will be assessed in response to different antigen packages delivered to the APC: recombinant Jug r 1 and Jug r 2, peptide fragments, whole purified proteins (albumins and large globulins), purified protein bodies (lectins and enzymes present), total walnut extract (pellicle polyphenolics and oil body lipids present), and the above protein sources with a quantified walnut total polyphenolic fraction added (rich in quercetin and ellagic acid). The above data will significantly advance our knowledge of the immunobiology of plant seed allergy.
Funding Source
Nat'l. Inst. of Allergy and Infectious Diseases
Project number
5K23AI001604-02
Categories
Chemical Contaminants
Parasites
Natural Toxins
Viruses and Prions
Bacterial Pathogens
Commodities
Nuts, Seeds